Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	746,183	A→G	intergenic (‑23469/‑41008)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	746,183	0	A	G	100.0%	213.5 / NA	71	intergenic (‑23469/‑41008)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/43);  total (28/43)

GCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTAAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGTTTT  >  1/745941‑746429                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag                                                                                                                                                                                                                                                  <  1:444364/249‑1 (MQ=255)      cAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTg                                                                                                                                                                                                                                           >  1:95087/1‑251 (MQ=255)        aTAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAACTGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGc                                                                                                                                                                                                                                          >  1:429114/1‑250 (MQ=255)                                   gCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTgg                                                                                                                                                                                                                <  1:318670/249‑1 (MQ=255)                                    cGAAAATTTTGATGCGCACCATTATGTTGCCATAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGAt                                                                                                                                                                                                              <  1:551083/250‑1 (MQ=255)                                         aTTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTgac                                                                                                                                                                                                        <  1:568931/251‑1 (MQ=255)                                                    cACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAg                                                                                                                                                                                                                        <  1:17105/224‑1 (MQ=255)                                                     aCCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt                                                                                                                                                                                            <  1:148430/251‑1 (MQ=255)                                                     aCCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt                                                                                                                                                                                            <  1:78520/251‑1 (MQ=255)                                                      ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag                                                                                                                                                                                                                                                  >  1:831827/1‑196 (MQ=255)                                                      ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattta                                                                                                                                                                                           >  1:831701/1‑251 (MQ=255)                                                      ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattta                                                                                                                                                                                           >  1:826713/1‑251 (MQ=255)                                                      ccATTATGTTGCCAAAGCTGCCGAACAAGGTCGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGt                                                                                                                                                                                                                                             <  1:447319/201‑1 (MQ=255)                                                              ttGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGc                                                                                                                                                                                   <  1:261257/251‑1 (MQ=255)                                                                       cTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac                                                                                                                                                                          <  1:205261/251‑1 (MQ=255)                                                                        tGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaaca                                                                                                                                                                         <  1:471499/251‑1 (MQ=255)                                                                            gAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCAttt                                                                                                                                                                                                                                  <  1:785541/190‑1 (MQ=255)                                                                            gAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGacgacg                                                                                                                                                                                                    >  1:648492/1‑220 (MQ=255)                                                                                  ggTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCgg                                                                                                                                                               >  1:102436/1‑251 (MQ=255)                                                                                   gTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAag                                                                                                                                                                                                                                                    <  1:666962/165‑1 (MQ=255)                                                                                      gtgtgGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGCGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTg                                                                                                                                                           >  1:327753/1‑251 (MQ=255)                                                                                                             ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAgaga                                                                                                                                                                                                                                                 <  1:149301/142‑1 (MQ=255)                                                                                                             ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaac                                                                                                                                    <  1:110248/251‑1 (MQ=255)                                                                                                             ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATCTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaac                                                                                                                                    <  1:3258/251‑1 (MQ=255)                                                                                                                   gAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaaca                                                                                                                              >  1:189855/1‑251 (MQ=255)                                                                                                                    aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac                                                                                                                               <  1:116805/249‑1 (MQ=255)                                                                                                                    aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac                                                                                                                               <  1:407789/249‑1 (MQ=255)                                                                                                                    aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac                                                                                                                               <  1:870923/249‑1 (MQ=255)                                                                                                                     aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTc                                                                                                                                                                                        >  1:522395/1‑191 (MQ=255)                                                                                                                     aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTc                                                                                                                                                                                        >  1:433026/1‑191 (MQ=255)                                                                                                                     aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCgg                                                                                                                                                               <  1:597986/216‑1 (MQ=255)                                                                                                                     aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGt                                                                                                                                         <  1:185744/238‑1 (MQ=255)                                                                                                                     aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaacag                                                                                                                             >  1:427394/1‑250 (MQ=255)                                                                                                                          atatCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATaaa                                                                                                                       <  1:670237/251‑1 (MQ=255)                                                                                                                          atatCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATaaa                                                                                                                       <  1:303221/251‑1 (MQ=255)                                                                                                                            atCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAAtt                                                                                                                     <  1:412146/251‑1 (MQ=255)                                                                                                                                    gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGc                                                                                                                                                                                                                               <  1:166471/137‑1 (MQ=255)                                                                                                                                    gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac                                                                                                                                                                          >  1:435014/1‑190 (MQ=255)                                                                                                                                    gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGa                                                                                                             >  1:687272/1‑251 (MQ=255)                                                                                                                                    gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGa                                                                                                             >  1:425906/1‑251 (MQ=255)                                                                                                                                         tCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGaa                                                                                                        >  1:512237/1‑251 (MQ=255)                                                                                                                                                aGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGgcg                                                                                                 >  1:171950/1‑251 (MQ=255)                                                                                                                                                   cAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCa                                                                                              >  1:418116/1‑251 (MQ=255)                                                                                                                                                      tcgtcTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATc                                                                                           <  1:395591/251‑1 (MQ=255)                                                                                                                                                          cTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCatat                                                                                       <  1:873422/251‑1 (MQ=255)                                                                                                                                                           ttGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATc                                                                                      <  1:668770/251‑1 (MQ=255)                                                                                                                                                             gCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGAt                                                                                                                                                                                                                                                <  1:598966/95‑1 (MQ=255)                                                                                                                                                                ttGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGa                                                                                 <  1:825058/251‑1 (MQ=255)                                                                                                                                                                       ggcTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac                                                                                                                               <  1:891557/198‑1 (MQ=255)                                                                                                                                                                            gCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag                                                                                                                                                                                                                                                  <  1:158076/78‑1 (MQ=255)                                                                                                                                                                            gCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac                                                                                                                                                                          >  1:773570/1‑150 (MQ=255)                                                                                                                                                                                          tGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGgcg                                                        <  1:807636/250‑1 (MQ=255)                                                                                                                                                                                           gAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacag                                                                                                                                  >  1:859302/1‑175 (MQ=255)                                                                                                                                                                                            aaaaaaTTAACCCGAAAGTGGTGGCTATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCaa                                                     >  1:875085/1‑251 (MQ=255)                                                                                                                                                                                                   tAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtg                                              <  1:768766/251‑1 (MQ=255)                                                                                                                                                                                                    aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt                                             <  1:316121/251‑1 (MQ=255)                                                                                                                                                                                                    aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt                                             <  1:500068/251‑1 (MQ=255)                                                                                                                                                                                                    aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt                                             <  1:848530/251‑1 (MQ=255)                                                                                                                                                                                                             gtggtgGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGt                                    >  1:500630/1‑251 (MQ=255)                                                                                                                                                                                                             gtggtgGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGt                                    >  1:873094/1‑251 (MQ=255)                                                                                                                                                                                                              tggtggCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTc                                   <  1:82227/251‑1 (MQ=255)                                                                                                                                                                                                                    cGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCaataa                              <  1:150403/250‑1 (MQ=255)                                                                                                                                                                                                                      aTGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGt                           <  1:487068/251‑1 (MQ=255)                                                                                                                                                                                                                      aTGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGt                           <  1:474562/251‑1 (MQ=255)                                                                                                                                                                                                                       tGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGc                                                                                                                                                                                                                     >  1:686230/1‑64 (MQ=255)                                                                                                                                                                                                                       tGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTac                                                                       <  1:579573/206‑1 (MQ=255)                                                                                                                                                                                                                          cGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATg                       >  1:302451/1‑251 (MQ=255)                                                                                                                                                                                                                           gggATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacag                                                                                                                                  <  1:437246/143‑1 (MQ=255)                                                                                                                                                                                                                              aTCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGa                                                                                 >  1:891681/1‑189 (MQ=255)                                                                                                                                                                                                                                        aaaaCCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt                                                                                                                                                                                            >  1:708359/1‑72 (MQ=255)                                                                                                                                                                                                                                               cGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTTGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGtttt  >  1:487136/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTAAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGTTTT  >  1/745941‑746429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑