Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	733,802	C→T	intergenic (‑11088/‑53389)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	733,802	0	C	T	100.0%	212.1 / NA	74	intergenic (‑11088/‑53389)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/46);  total (28/46)

GCGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATACCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGCTGGGCAACGAACAA  >  1/733558‑734046                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCatta                                                                                                                                                                                                                                                  <  1:299023/250‑1 (MQ=255) gCGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCatta                                                                                                                                                                                                                                                  <  1:243836/250‑1 (MQ=255)  cGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGTGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCattat                                                                                                                                                                                                                                                 <  1:211268/250‑1 (MQ=255)  cGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCattat                                                                                                                                                                                                                                                 <  1:739769/250‑1 (MQ=255)        tGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGaa                                                                                                                                                                                                                                          <  1:235811/251‑1 (MQ=255)                   cTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATaa                                                                                                                                                                                                                               <  1:620461/251‑1 (MQ=255)                          gCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTTTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGGTTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCCTTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAAc                                                                                                                                                                                                                                         >  1:95647/1‑234 (MQ=255)                          gCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGGGATGTCGGGTTTTTATCATCAACGTTATAACGTGTTGGTGGGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAAc                                                                                                                                                                                                                                         >  1:510319/1‑234 (MQ=255)                                 ggTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGgcg                                                                                                                                                                                                                  >  1:195757/1‑250 (MQ=255)                                 ggTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGgcg                                                                                                                                                                                                                  >  1:119701/1‑250 (MQ=255)                                 ggTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGgcg                                                                                                                                                                                                                  >  1:678018/1‑250 (MQ=255)                                  gtgtATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTg                                                                                                                                                                                                                                            <  1:361261/223‑1 (MQ=255)                                  gtgtATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGaa                                                                                                                                                                                                                                          >  1:233422/1‑225 (MQ=255)                                      attattTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGc                                                                                                                                                                                                                        <  1:713299/239‑1 (MQ=255)                                          tttGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACAcc                                                                                                                                                                                                        <  1:417487/251‑1 (MQ=255)                                          tttGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACAcc                                                                                                                                                                                                        <  1:77926/251‑1 (MQ=255)                                             gCATAACGATGTGGCGAGCATTGAAAATACGGCGGCAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATaa                                                                                                                                                                                                                               >  1:897786/1‑225 (MQ=255)                                               aTAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCtt                                                                                                                                                                                                   <  1:126702/251‑1 (MQ=255)                                               aTAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCtt                                                                                                                                                                                                   <  1:186483/251‑1 (MQ=255)                                               aTAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCtt                                                                                                                                                                                                   <  1:243784/251‑1 (MQ=255)                                                   cGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑g                                                                                                                                                                                              <  1:696970/251‑1 (MQ=255)                                                      tgtgGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGc                                                                                                                                                                                           <  1:828245/251‑1 (MQ=255)                                                             aGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAg                                                                                                                                                                                     <  1:631796/250‑1 (MQ=255)                                                             aGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAg                                                                                                                                                                                     <  1:606637/250‑1 (MQ=255)                                                                aTTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCg                                                                                                                                                                                 <  1:587462/251‑1 (MQ=255)                                                                aTTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCg                                                                                                                                                                                 <  1:543430/251‑1 (MQ=255)                                                                     aaaTACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGc                                                                                                                                                                                                     <  1:56906/227‑1 (MQ=255)                                                                            gcggAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTg                                                                                                                                                                                                                                            >  1:561426/1‑181 (MQ=255)                                                                                aaaaaCTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAgcc                                                                                                                                                                 <  1:638282/251‑1 (MQ=255)                                                                                aaaaaCTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAgcc                                                                                                                                                                 <  1:346374/251‑1 (MQ=255)                                                                                aaaaaCTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAgcc                                                                                                                                                                 <  1:693035/251‑1 (MQ=255)                                                                                   aaCTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTg                                                                                                                                                                                                                                            <  1:692069/174‑1 (MQ=255)                                                                                                           cggcgcgtcccggtgggCACGGGCAAATGGGGGGGCGGGAGCTGGAACGCGTGAAGTCCGATTTTTAACAACAAAGTTAAAACGTGTTGGGGGTTTCTTACATTTATGAAACGGGCATCCATGTGGCGGCCGCTAATATCATTATTATTTAACGGGCCGAAAAATTCGGGTTGGCGGCGTTTAACCAGGCTCGTGGGGCGGGAAGGG‑GTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCcgccgc                                                                                                                                        <  1:843904/235‑1 (MQ=255)                                                                                                               cgTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAAc                                                                                                                                  <  1:419344/251‑1 (MQ=255)                                                                                                                 tCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCaaaa                                                                                                                                   >  1:15922/1‑248 (MQ=255)                                                                                                                 tCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCaaaa                                                                                                                                   >  1:22346/1‑248 (MQ=255)                                                                                                                    ccGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACtgatg                                                                                                                             <  1:235825/251‑1 (MQ=255)                                                                                                                      gTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCattattat                                                                                                                                                                                                                                              <  1:471085/137‑1 (MQ=255)                                                                                                                          ggCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCaa                                                                                                                        <  1:766394/250‑1 (MQ=255)                                                                                                                               gggCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAgcctatgcc                                                                                                                                                           <  1:46587/210‑1 (MQ=255)                                                                                                                                            aGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTacac                                                                                                                                                                                                         >  1:221861/1‑152 (MQ=255)                                                                                                                                             gCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAAGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAAAGTTTAg                                                                                                    >  1:454048/1‑251 (MQ=255)                                                                                                                                                 gAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAgcag                                                                                                 >  1:787471/1‑247 (MQ=255)                                                                                                                                                          cgcgTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGaa                                                                                         <  1:132849/249‑1 (MQ=255)                                                                                                                                                          cgcgTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGaa                                                                                         <  1:267342/249‑1 (MQ=255)                                                                                                                                                                   tCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATa                                                                               <  1:376169/250‑1 (MQ=255)                                                                                                                                                                   tCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATa                                                                               <  1:355633/250‑1 (MQ=255)                                                                                                                                                                      gATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCattattat                                                                                                                                                                                                                                              >  1:90783/1‑89 (MQ=255)                                                                                                                                                                      gATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAAcc                                                                            <  1:887028/250‑1 (MQ=255)                                                                                                                                                                            tATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGgggc                                                                     <  1:780341/251‑1 (MQ=255)                                                                                                                                                                            tATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGgggc                                                                     <  1:541984/251‑1 (MQ=255)                                                                                                                                                                                  caACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCa                                                                                                                                                                                                       >  1:407643/1‑116 (MQ=255)                                                                                                                                                                                    aCGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGAt                                                                                >  1:10684/1‑232 (MQ=255)                                                                                                                                                                                    aCGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGgggc                                                                     <  1:172718/243‑1 (MQ=255)                                                                                                                                                                                     cGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCAt                                                            >  1:136111/1‑251 (MQ=255)                                                                                                                                                                                      gTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCAt                                                            >  1:223276/1‑250 (MQ=255)                                                                                                                                                                                           aaCGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAgcctatgcc                                                                                                                                                           <  1:348344/150‑1 (MQ=255)                                                                                                                                                                                              gtgtTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGAcc                                                                                                                          <  1:330523/180‑1 (MQ=255)                                                                                                                                                                                              gtgtTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGaa                                                                                         <  1:25567/213‑1 (MQ=255)                                                                                                                                                                                              gtgtTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCaa                                                    >  1:328914/1‑250 (MQ=255)                                                                                                                                                                                              gtgtTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCaa                                                    >  1:706615/1‑250 (MQ=255)                                                                                                                                                                                              gtgtTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGA‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGAt                                                                                <  1:804262/222‑1 (MQ=255)                                                                                                                                                                                                      gtgtTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGAtt                                           >  1:356200/1‑251 (MQ=255)                                                                                                                                                                                                        gtTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTg                                         >  1:877394/1‑251 (MQ=255)                                                                                                                                                                                                          tCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGa                                       <  1:821484/251‑1 (MQ=255)                                                                                                                                                                                                                 ttattGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAg                                                                                                                                                                                     <  1:606516/102‑1 (MQ=255)                                                                                                                                                                                                                   attGAAACGGGCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCgtgg                              >  1:415754/1‑251 (MQ=255)                                                                                                                                                                                                                            ggCATCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCg                      <  1:253166/250‑1 (MQ=255)                                                                                                                                                                                                                                tCGATGTGCCGACCGCTAATATCATTATTATTGAATGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAACGACGCGCAAACACGTTTAGAAGCCTTGGCAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGAGCCGGCGAATTg                 >  1:742290/1‑251 (MQ=255)                                                                                                                                                                                                                                tCGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTg                 >  1:109933/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGATGTGCCGACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGc                <  1:245047/251‑1 (MQ=255)                                                                                                                                                                                                                                          gACCGCTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGCTgggtcagt       >  1:49688/1‑246 (MQ=255)                                                                                                                                                                                                                                               cTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGCTGGGCAACGAACaa  >  1:234554/1‑251 (MQ=255)                                                                                                                                                                                                                                               cTAATATCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGCTGGGCAACGAACaa  >  1:11368/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCGATTTTGCGTGAAATTCTGCGTGGCGGGCAGGTGTATTATTTGCATAACGATGTGGCGAGCATTGAAAATACGGCGGAAAAACTGACCGCACTTGTGCCGGAAGCGCGCGTCACCGTGGGGCACGGGCAAATGCGCGAGCGGGAGCTGGAGCGCGTGATGTCGGATTTTTATCATCAACGTTATAACGTGTTGGTGTGTTCTACCATTATTGAAACGGGCATCGATGTGCCGACCGCTAATACCATTATTATTGAACGCGCCGATAATTTCGGCTTGGCGCAGTTACACCAGCTTCGT‑GGGCGAGTAGGGCGTTCCCATCATCAAGCCTATGCCTATTTGCTCACTCCGCCGCCAAAACTGATGACCAAAGACGCGCAAAAACGTTTAGAAGCCTTGGAAAGTCTGGATAACCTCGGGGCGGGCTTCATCTTGGCAACCCACGATTTGGAAATCCGTGGTGCCGGCGAATTGCTGGGCAACGAACAA  >  1/733558‑734046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑