Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	731,035	C→T	intergenic (‑8321/‑56156)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	731,035	0	C	T	100.0%	139.3 / NA	51	intergenic (‑8321/‑56156)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/24);  total (27/24)

GGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGCGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGTGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGACGGTCTAACCGTG  >  1/730790‑731271                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             tgggcaaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                                                                                        <  1:824935/245‑1 (MQ=14)      aaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                                                                                                                                                   <  1:679621/250‑1 (MQ=255)             aaaTTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTa                                                                                                                                                                                                                            <  1:621061/250‑1 (MQ=255)                              cTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCATTGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGa                                                                                                                                                                                                           >  1:686507/1‑251 (MQ=255)                                       aCATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTg                                                                                                                                                                                                  <  1:807303/251‑1 (MQ=255)                                        cATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAAt                                                                                                                                                                                                                              >  1:551652/1‑222 (MQ=255)                                             aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTtata                                                                                                                                                                                                               >  1:520546/1‑232 (MQ=255)                                             aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAAt                                                                                                                                                                                             >  1:357275/1‑250 (MQ=255)                                                                             ttATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAAc                                                                                                                                                                         <  1:754674/238‑1 (MQ=21)                                                                               aTTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                                                                                        >  1:540947/1‑173 (MQ=255)                                                                                       gTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                                                                                                                                                   >  1:454584/1‑250 (MQ=25)                                                                                        ttCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                                                                                                                                                   >  1:123398/1‑249 (MQ=25)                                                                                                            ccccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAActg                                                                                                                                                                   >  1:440748/1‑211 (MQ=25)                                                                                                              ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                                                                                                                                                   <  1:629812/147‑1 (MQ=18)                                                                                                              ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTgg                                                                                                                                                                                                 >  1:285552/1‑181 (MQ=255)                                                                                                              ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCa                                                                                                                           >  1:598920/1‑251 (MQ=37)                                                                                                                                 agCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATAc                                                                                                                                                                                                              >  1:590411/1‑149 (MQ=21)                                                                                                                                  gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                                                                                        >  1:16384/1‑122 (MQ=18)                                                                                                                                  gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTAt                                                                                                                                                                                                    <  1:607509/158‑1 (MQ=25)                                                                                                                                             aaaCGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAgtg                                                                                            <  1:94864/251‑3 (MQ=38)                                                                                                                                                      gCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                                                                                        >  1:616685/1‑102 (MQ=18)                                                                                                                                                            tGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAAtt                                                                             <  1:793962/251‑1 (MQ=39)                                                                                                                                                                  cccGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGAt                                                                                      >  1:616048/1‑236 (MQ=39)                                                                                                                                                                  cccGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGc                                                                        >  1:790940/1‑250 (MQ=255)                                                                                                                                                                         aCGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATcc                                                                <  1:849332/251‑1 (MQ=255)                                                                                                                                                                                           atttaaaaaaTATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGc                                               <  1:361648/247‑1 (MQ=255)                                                                                                                                                                                                      atCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAg                                    >  1:108388/1‑251 (MQ=255)                                                                                                                                                                                                        cAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCg                                  <  1:292915/251‑1 (MQ=255)                                                                                                                                                                                                            gAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGt                                                                                                                                            <  1:707718/141‑1 (MQ=255)                                                                                                                                                                                                            gAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAAtt                                                                                                                        <  1:583595/161‑1 (MQ=255)                                                                                                                                                                                                              aTTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTg                                                                                                                                                                                                                         <  1:206894/62‑1 (MQ=255)                                                                                                                                                                                                              aTTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGccc                                                           >  1:749444/1‑220 (MQ=255)                                                                                                                                                                                                              aTTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCa                                        <  1:241638/239‑1 (MQ=255)                                                                                                                                                                                                              aTTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCg                                  <  1:753180/245‑1 (MQ=255)                                                                                                                                                                                                                 ataATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                                                                                                                                                   >  1:538236/1‑49 (MQ=255)                                                                                                                                                                                                                 ataATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGc                                               <  1:241559/229‑1 (MQ=255)                                                                                                                                                                                                                              caacTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAACCTCAATTTTTTCATCTCGAAATTCTTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGAATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGatgg            >  1:435030/1‑248 (MQ=255)                                                                                                                                                                                                                                 cTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGttt         >  1:364516/1‑249 (MQ=255)                                                                                                                                                                                                                                 cTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGttt         >  1:317448/1‑249 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATAc                                                                                                                                                                                                              <  1:1677/52‑1 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTa                                                                                                                                                                       <  1:526782/91‑1 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAAtttt                                                                                                                      >  1:772607/1‑140 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTAccc                                                                                                 >  1:822983/1‑161 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATc                                                                 <  1:296127/193‑1 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGAt                                                  <  1:564625/208‑1 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGTTTaa       >  1:500241/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGTTTaa       >  1:14436/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGTTTAACCg    <  1:628819/251‑1 (MQ=255)                                                                                                                                                                                                                                        aCCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTAcc                                                                                                  >  1:749997/1‑155 (MQ=255)                                                                                                                                                                                                                                        aCCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGCGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGATGGTTTAACCgtg  >  1:121391/1‑251 (MQ=255)                                                                                                                                                                                                                                             gggCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAatat                                                                  <  1:705800/181‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             GGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGCGAATGATCATAAAATTTTAGCCAATATCCTGCCCGGTGCGGATAGCCTCGCCATTAGCGAAATTGCCGCGCAGTTTGACGGTCTAACCGTG  >  1/730790‑731271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑