Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,924	C→T	intergenic (‑8210/‑56267)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,924	0	C	T	100.0%	140.1 / NA	60	intergenic (‑8210/‑56267)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/31);  total (29/31)

CTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCA  >  1/730682‑731150                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   cTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCaaaa                                                                                                                                                                                                                            >  1:901487/1‑251 (MQ=17)                ggtattacagttGTTCGAAACAATGTAACTATTGATATAAAAAGAAAAAACTTGTCTCCCCCCGTTCACGGGGGTGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAATCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGt                                                                                                                                                                                                                  <  1:804899/235‑1 (MQ=14)                  gggCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTc                                                                                                                                                                                                            >  1:789554/1‑250 (MQ=14)                       caACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCg                                                                                                                                                                                                      >  1:225292/1‑251 (MQ=14)                            gTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTaaa                                                                                                                                                                                                  >  1:596625/1‑250 (MQ=14)                            gTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTaaa                                                                                                                                                                                                  >  1:711883/1‑250 (MQ=14)                             ttCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAAc                                                                                                                                                                                                 <  1:415945/250‑1 (MQ=14)                                 aaaCATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACggagg                                                                                                                                                                                            <  1:492951/251‑1 (MQ=14)                                      ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGtt                                                                                                                                                                                                                 >  1:565987/1‑225 (MQ=14)                                      ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                                                                                                                                       <  1:254037/251‑1 (MQ=14)                                      ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                                                                                                                                       <  1:485354/251‑1 (MQ=14)                                      ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                                                                                                                                       <  1:742567/251‑1 (MQ=14)                                      ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                                                                                                                                       <  1:159791/251‑1 (MQ=14)                                        gTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCaa                                                                                                                                                                                     >  1:507934/1‑251 (MQ=14)                                        gTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCaa                                                                                                                                                                                     >  1:850765/1‑251 (MQ=14)                                                  atataAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                                                                                                                                           <  1:252/251‑1 (MQ=14)                                                               aaaCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGaa                                                                                                                                                             <  1:62052/251‑1 (MQ=14)                                                                 aCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAAtt                                                                                                                                                           >  1:879846/1‑251 (MQ=14)                                                                       ctCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATg                                                                                                                                                     <  1:261645/250‑1 (MQ=14)                                                                             ccGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGa                                                                                                                                               >  1:6479/2‑251 (MQ=14)                                                                               gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAAc                                                                                                                                                                                                 <  1:212281/200‑1 (MQ=14)                                                                               gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                                                                                                                                       >  1:159695/1‑210 (MQ=255)                                                                               gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                                                                                                                                       <  1:389946/241‑1 (MQ=14)                                                                                 tCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGaacaa                                                                                                                                           >  1:668103/1‑251 (MQ=255)                                                                                  cACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaaaa                                                                                                                                                                         <  1:598963/221‑1 (MQ=255)                                                                                      ggggAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTa                                                                                                                                      >  1:740927/1‑251 (MQ=255)                                                                                            cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAccc                                                                                                                                <  1:392023/251‑1 (MQ=255)                                                                                            cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAc                                                                                                                                  <  1:558716/249‑1 (MQ=14)                                                                                                 cGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGc                                                                                                                           <  1:493436/251‑1 (MQ=14)                                                                                                    aGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                                                                                                                                           <  1:329334/201‑1 (MQ=255)                                                                                                     gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                                                                                                                                       <  1:818221/219‑1 (MQ=11)                                                                                                     gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg                                                                                                                       >  1:368847/1‑251 (MQ=14)                                                                                                      cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACTCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg                                                                                                                       >  1:126165/1‑250 (MQ=14)                                                                                                      cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                      <  1:845409/251‑1 (MQ=14)                                                                                                      cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                      <  1:50152/251‑1 (MQ=14)                                                                                                      cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                      <  1:705025/251‑1 (MQ=14)                                                                                                             tgggcaaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                               <  1:824935/245‑1 (MQ=14)                                                                                                               ggcaaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGat                                                                                                                                                                                  <  1:770942/179‑1 (MQ=255)                                                                                                                  aaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                          <  1:679621/250‑1 (MQ=255)                                                                                                                     aTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGat                                                                                                                                                                                  >  1:276756/1‑177 (MQ=255)                                                                                                                         aaaTTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTa                                                                                                   <  1:621061/250‑1 (MQ=255)                                                                                                                                 aaGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAAc                                                                                                                                                                                                 <  1:569635/150‑1 (MQ=255)                                                                                                                                 aaGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGttt                                                                                                                                                  >  1:87613/1‑196 (MQ=255)                                                                                                                                          cTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCATTGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGa                                                                                  >  1:686507/1‑251 (MQ=255)                                                                                                                                                   aCATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTg                                                                         <  1:807303/251‑1 (MQ=255)                                                                                                                                                    cATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGaac                                                                                                                                             <  1:694365/182‑1 (MQ=255)                                                                                                                                                    cATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAAt                                                                                                     >  1:551652/1‑222 (MQ=255)                                                                                                                                                         aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTtata                                                                                      >  1:520546/1‑232 (MQ=255)                                                                                                                                                         aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAAt                                                                    >  1:357275/1‑250 (MQ=255)                                                                                                                                                                                         ttATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAAc                                                <  1:754674/238‑1 (MQ=21)                                                                                                                                                                                           aTTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                               >  1:540947/1‑173 (MQ=255)                                                                                                                                                                                                   gTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                          >  1:454584/1‑250 (MQ=25)                                                                                                                                                                                                    ttCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                          >  1:123398/1‑249 (MQ=25)                                                                                                                                                                                                                        ccccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAActg                                          >  1:440748/1‑211 (MQ=25)                                                                                                                                                                                                                          ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                          <  1:629812/147‑1 (MQ=18)                                                                                                                                                                                                                          ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTgg                                                                        >  1:285552/1‑181 (MQ=255)                                                                                                                                                                                                                          ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCa  >  1:598920/1‑251 (MQ=37)                                                                                                                                                                                                                                             agCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATAc                                                                                     >  1:590411/1‑149 (MQ=21)                                                                                                                                                                                                                                              gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                               >  1:16384/1‑122 (MQ=18)                                                                                                                                                                                                                                              gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTAt                                                                           <  1:607509/158‑1 (MQ=25)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   CTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCA  >  1/730682‑731150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑