Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,790	G→T	intergenic (‑8076/‑56401)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,790	0	G	T	100.0%	133.1 / NA	63	intergenic (‑8076/‑56401)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/35);  total (28/35)

TCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGC  >  1/730563‑731034                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGc                                                                                                                                                                                                                               <  1:14479/251‑1 (MQ=25)                       gtgTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgtgggca                                                                                                                                                                                                                                                 <  1:605292/209‑3 (MQ=37)                       gtgTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAg                                                                                                                                                                                                         >  1:365343/3‑251 (MQ=21)                          tGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGAt                                                                                                                                                                                                       <  1:570497/250‑1 (MQ=21)                                      cTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCtt                                                                                                                                                                                           >  1:737887/1‑250 (MQ=21)                                        tGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCg                                                                                                                                                                                        >  1:704721/1‑251 (MQ=21)                                          ccggggCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGcc                                                                                                                                                                                      <  1:233468/247‑1 (MQ=21)                                           cggggCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGccc                                                                                                                                                                                     <  1:526883/248‑1 (MQ=21)                                              ggCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCtt                                                                                                                                                                                   <  1:454825/250‑1 (MQ=21)                                                  gCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCggg                                                                                                                                                                               >  1:270103/1‑250 (MQ=18)                                                     tgccGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGACAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGTTCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACg                                                                                                                                                                           >  1:424490/3‑251 (MQ=18)                                                       ccGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCTGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGt                                                                                                                                                                          <  1:452456/250‑1 (MQ=18)                                                         ggtggtGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAt                                                                                                                                                                       >  1:837115/1‑251 (MQ=18)                                                                             aTCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGAt                                                                                                                                                                                                       >  1:762346/1‑199 (MQ=18)                                                                              tcctgaaccgaaAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGAt                                                                                                                                                                                                       >  1:843753/5‑198 (MQ=18)                                                                              tcctgaaccgaaAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAacttact                                                                                                                                                  <  1:55448/247‑1 (MQ=18)                                                                                     ccgaaAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCAc                                                                                                                                           <  1:109165/251‑1 (MQ=18)                                                                                                aaCGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTggggg                                                                                                                                <  1:65322/251‑1 (MQ=17)                                                                                                             aaagaaaCGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGcc                                                                                                                   <  1:159978/251‑1 (MQ=17)                                                                                                                        cTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCaaaa                                                                                                        >  1:901487/1‑251 (MQ=17)                                                                                                                                    ccccGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATccc                                                                                                                                                                                                >  1:105131/2‑151 (MQ=17)                                                                                                                                       ggtattacagttGTTCGAAACAATGTAACTATTGATATAAAAAGAAAAAACTTGTCTCCCCCCGTTCACGGGGGTGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTAATCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGt                                                                                              <  1:804899/235‑1 (MQ=14)                                                                                                                                         gggCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGAt                                                                                                                                                                                                       >  1:209461/1‑139 (MQ=17)                                                                                                                                         gggCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTc                                                                                        >  1:789554/1‑250 (MQ=14)                                                                                                                                              caACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCg                                                                                  >  1:225292/1‑251 (MQ=14)                                                                                                                                                   gTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTaaa                                                                              >  1:711883/1‑250 (MQ=14)                                                                                                                                                   gTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTaaa                                                                              >  1:596625/1‑250 (MQ=14)                                                                                                                                                    ttCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAAc                                                                             <  1:415945/250‑1 (MQ=14)                                                                                                                                                        aaaCATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACggagg                                                                        <  1:492951/251‑1 (MQ=14)                                                                                                                                                          aCATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCAGccc                                                                                                                                                                                     <  1:895663/140‑1 (MQ=14)                                                                                                                                                           cATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCa                                                                                                                                                                  >  1:394366/1‑158 (MQ=14)                                                                                                                                                           cATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAac                                                                                                                                                       <  1:97420/169‑1 (MQ=14)                                                                                                                                                           cATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCAccc                                                                                                                                         <  1:9202/183‑1 (MQ=14)                                                                                                                                                           cATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGcc                                                                                                                   <  1:554798/205‑1 (MQ=14)                                                                                                                                                             ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGtt                                                                                             >  1:565987/1‑225 (MQ=14)                                                                                                                                                             ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                   <  1:485354/251‑1 (MQ=14)                                                                                                                                                             ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                   <  1:742567/251‑1 (MQ=14)                                                                                                                                                             ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                   <  1:254037/251‑1 (MQ=14)                                                                                                                                                             ttGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                   <  1:159791/251‑1 (MQ=14)                                                                                                                                                               gTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCaa                                                                 >  1:507934/1‑251 (MQ=14)                                                                                                                                                               gTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCaa                                                                 >  1:850765/1‑251 (MQ=14)                                                                                                                                                                   cTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGcc                                                                                                                   >  1:840140/1‑197 (MQ=14)                                                                                                                                                                         atataAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                       <  1:252/251‑1 (MQ=14)                                                                                                                                                                                      aaaCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGaa                                         <  1:62052/251‑1 (MQ=14)                                                                                                                                                                                        aCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAAtt                                       >  1:879846/1‑251 (MQ=14)                                                                                                                                                                                              ctCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATg                                 <  1:261645/250‑1 (MQ=14)                                                                                                                                                                                                    ccGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGa                           >  1:6479/2‑251 (MQ=14)                                                                                                                                                                                                      gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACg                                                                                                                                                                           >  1:295584/1‑106 (MQ=11)                                                                                                                                                                                                      gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAAc                                                                             <  1:212281/200‑1 (MQ=14)                                                                                                                                                                                                      gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGc                                                                   >  1:159695/1‑210 (MQ=255)                                                                                                                                                                                                      gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                   <  1:389946/241‑1 (MQ=14)                                                                                                                                                                                                        tCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGaacaa                       >  1:668103/1‑251 (MQ=255)                                                                                                                                                                                                         cACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaaaa                                                     <  1:598963/221‑1 (MQ=255)                                                                                                                                                                                                             ggggAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTa                  >  1:740927/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAccc            <  1:392023/251‑1 (MQ=255)                                                                                                                                                                                                                   cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAc              <  1:558716/249‑1 (MQ=14)                                                                                                                                                                                                                        cGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGc       <  1:493436/251‑1 (MQ=14)                                                                                                                                                                                                                           aGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                       <  1:329334/201‑1 (MQ=255)                                                                                                                                                                                                                            gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                   <  1:818221/219‑1 (MQ=11)                                                                                                                                                                                                                            gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg   >  1:368847/1‑251 (MQ=14)                                                                                                                                                                                                                             cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACTCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg   >  1:126165/1‑250 (MQ=14)                                                                                                                                                                                                                             cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc  <  1:845409/251‑1 (MQ=14)                                                                                                                                                                                                                             cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc  <  1:50152/251‑1 (MQ=14)                                                                                                                                                                                                                             cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc  <  1:705025/251‑1 (MQ=14)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGC  >  1/730563‑731034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑