Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,586	C→T	intergenic (‑7872/‑56605)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,586	0	C	T	100.0%	164.8 / NA	59	intergenic (‑7872/‑56605)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/37);  total (22/37)

AGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGC  >  1/730344‑730813                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    aacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                                                                              <  1:114500/247‑1 (MQ=255)           tCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAgg                                                                                                                                                                                                                          >  1:651044/1‑245 (MQ=255)                         ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                                                                                      <  1:580848/251‑3 (MQ=255)                         ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtg                                                                                                                                                                                                       >  1:728463/1‑249 (MQ=255)                          cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                                                                                      >  1:765256/1‑248 (MQ=255)                          cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                                                                                      >  1:746828/1‑248 (MQ=255)                            aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg                                                                                                                                                                                                   >  1:837236/1‑251 (MQ=255)                            aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg                                                                                                                                                                                                   >  1:232882/1‑251 (MQ=255)                               gcaagcgtccTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCggtgg                                                                                                                                                                                                >  1:537030/9‑251 (MQ=255)                                     gtccTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                                                                              <  1:385174/213‑1 (MQ=255)                                          gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGttcga                                                                                                                                                                                      >  1:792636/1‑247 (MQ=255)                                          gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGttcga                                                                                                                                                                                      >  1:589438/1‑247 (MQ=255)                                               cATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGc                                                                                                                                                                                                             >  1:222198/1‑222 (MQ=255)                                                  tCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATcc                                                                                                                                                                             <  1:830768/251‑1 (MQ=255)                                                       aTAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCctgaac                                                                                                                                                                        <  1:653584/251‑1 (MQ=255)                                                                             ttGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTAGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCggagaa                                                                                                                                                  >  1:689557/1‑249 (MQ=255)                                                                                       gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAAc                                                                                                                                         <  1:301853/250‑1 (MQ=255)                                                                                       gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACg                                                                                                                                        >  1:678144/1‑251 (MQ=255)                                                                                            gaagaaAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                                                                              <  1:28530/160‑1 (MQ=255)                                                                                                      ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                                                                         <  1:603870/251‑2 (MQ=255)                                                                                                      ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                                                                         <  1:283630/251‑2 (MQ=255)                                                                                                                aTTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGcac                                                                                                               <  1:74588/251‑1 (MQ=255)                                                                                                                   tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                                                                         <  1:456691/238‑2 (MQ=255)                                                                                                                   tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                            <  1:361764/251‑1 (MQ=255)                                                                                                                   tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                            <  1:519951/251‑1 (MQ=255)                                                                                                                   tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                            <  1:486776/251‑1 (MQ=255)                                                                                                                        ttcttATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCggaga                                                                                                                           <  1:674734/231‑4 (MQ=255)                                                                                                                            tATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCAc                                                                                                                                     <  1:517075/217‑1 (MQ=255)                                                                                                                             aTGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAAc                                                                                                  <  1:124046/251‑1 (MQ=255)                                                                                                                               gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                            >  1:323701/1‑239 (MQ=255)                                                                                                                               gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACAt                                                                                                >  1:644077/1‑251 (MQ=255)                                                                                                                                     aacCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAAc                                                                                          <  1:338368/251‑1 (MQ=255)                                                                                                                                      acCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                            >  1:465414/1‑200 (MQ=255)                                                                                                                                         caaaaTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTAtt                                                                                      <  1:237814/248‑1 (MQ=255)                                                                                                                                           aaaTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                            <  1:67888/194‑1 (MQ=255)                                                                                                                                             aTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTtata                                                                                  <  1:48770/251‑1 (MQ=255)                                                                                                                                                         cGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGaaaaa                                                                      >  1:557630/1‑251 (MQ=255)                                                                                                                                                            cTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACt                                                                   <  1:243630/251‑1 (MQ=255)                                                                                                                                                                ggaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTgg                                                                >  1:497088/1‑250 (MQ=255)                                                                                                                                                                 gaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTggc                                                               <  1:151698/250‑2 (MQ=255)                                                                                                                                                                   ggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                                                                            >  1:75581/1‑203 (MQ=255)                                                                                                                                                                     caaatATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTccc                                                           >  1:122096/5‑250 (MQ=255)                                                                                                                                                                      aaatATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCggag                                                                                                                            <  1:520510/181‑3 (MQ=255)                                                                                                                                                                           tgacTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                                                                            <  1:27244/160‑1 (MQ=255)                                                                                                                                                                            gacTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACgg                                                                                                                                                         <  1:755964/147‑1 (MQ=255)                                                                                                                                                                                     agctaaaaaaGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCg                                                                                                                                                                                                                 <  1:441357/80‑1 (MQ=255)                                                                                                                                                                                     agctaaaaaaGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGaa                                                                                                    <  1:20633/189‑1 (MQ=255)                                                                                                                                                                                     agctaaaaaaGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAg                                           >  1:764416/5‑250 (MQ=255)                                                                                                                                                                                          aaaaaGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGc                                     <  1:212320/251‑1 (MQ=255)                                                                                                                                                                                                    gttAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAAc                                                                                          >  1:169392/1‑188 (MQ=255)                                                                                                                                                                                                    gttAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTAtt                                                                                      <  1:741655/192‑1 (MQ=255)                                                                                                                                                                                                      tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgt                         <  1:590924/251‑2 (MQ=255)                                                                                                                                                                                                      tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgt                         <  1:692890/251‑2 (MQ=255)                                                                                                                                                                                                      tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgt                         <  1:494128/251‑2 (MQ=255)                                                                                                                                                                                                      tAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgt                         <  1:532025/251‑2 (MQ=255)                                                                                                                                                                                                                  gggATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTa                                                                                        <  1:473958/176‑1 (MQ=255)                                                                                                                                                                                                                  gggATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGc                                     <  1:36691/227‑1 (MQ=255)                                                                                                                                                                                                                      tgcctATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAgt                         >  1:329222/5‑234 (MQ=37)                                                                                                                                                                                                                             tCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGc  <  1:14479/251‑1 (MQ=25)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    AGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGC  >  1/730344‑730813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑