Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,515	G→A	intergenic (‑7801/‑56676)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,515	0	G	A	100.0%	208.0 / NA	72	intergenic (‑7801/‑56676)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/40);  total (32/40)

TTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCC  >  1/730282‑730756                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  tttCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTaaaaa                                                                                                                                                                                                                                   >  1:599641/1‑251 (MQ=255)   tcttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTgaag                                                                                                                                                                                                                                             <  1:809826/239‑1 (MQ=255)    cttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAgg                                                                                                                                                                                                                                >  1:164548/1‑251 (MQ=255)     ttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                                                                                                                                                                               <  1:50446/251‑1 (MQ=255)           ccGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTa                                                                                                                                                                                                                         >  1:848823/1‑251 (MQ=255)                 gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                                                                                                                                                                                    <  1:497927/250‑1 (MQ=255)                  cGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcgcg                                                                                                                                                                                                                  >  1:716448/1‑251 (MQ=255)                       gtcttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTgg                                                                                                                                                                                                             >  1:490986/3‑251 (MQ=255)                         cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                                                                                                                                                           <  1:394941/251‑1 (MQ=255)                         cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                                                                                                                                                           <  1:198214/251‑1 (MQ=255)                         cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGAGGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                                                                                                                                                           <  1:435084/251‑1 (MQ=255)                              ccGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTaa                                                                                                                                                                                                                        <  1:543942/233‑1 (MQ=255)                                 cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                                                                                                                                                                                   >  1:243985/1‑251 (MQ=255)                                 cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                                                                                                                                                                                   >  1:474711/1‑251 (MQ=255)                                   aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAGTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                                                                                                                                                                               <  1:118271/221‑1 (MQ=255)                                   aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTcc                                                                                                                                                                                                 <  1:25708/251‑1 (MQ=255)                                     tCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGa                                                                                                                                                                                               <  1:730850/251‑1 (MQ=255)                                                  aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGccta                                                                                                                                                                                                     >  1:115330/5‑229 (MQ=255)                                                  aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaaga                                                                                                                                                                                   <  1:674475/246‑1 (MQ=255)                                                   aagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaagaag                                                                                                                                                                                 >  1:766491/4‑251 (MQ=255)                                                          cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt                                                                                                                                                                          <  1:697933/251‑7 (MQ=255)                                                          cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt                                                                                                                                                                          <  1:280371/251‑7 (MQ=255)                                                              gaacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAACAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                                                                                                                                                                                    >  1:516088/6‑205 (MQ=255)                                                               aacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                     <  1:114500/247‑1 (MQ=255)                                                                         tCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAgg                                                                                                                                                                 >  1:651044/1‑245 (MQ=255)                                                                          ccTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                                                                                                                                                           >  1:6763/1‑202 (MQ=255)                                                                           cTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTc                                                                                                                                                                                                  <  1:155664/210‑1 (MQ=255)                                                                               aTTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGc                                                                                                                                                                                        <  1:674247/216‑1 (MQ=255)                                                                                      gCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAACATAAAACGGCATCGACTAAGGAGGCAAATACGACTGAAGAAGCTAAAAAAGGTTGGCTAAAACGCGCTTgggcagcc                                                                                                                                                                                                       >  1:700849/1‑189 (MQ=255)                                                                                       ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                             <  1:580848/251‑3 (MQ=255)                                                                                       ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtg                                                                                                                                              >  1:728463/1‑249 (MQ=255)                                                                                        cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                             >  1:765256/1‑248 (MQ=255)                                                                                        cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc                                                                                                                                             >  1:746828/1‑248 (MQ=255)                                                                                          aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg                                                                                                                                          >  1:837236/1‑251 (MQ=255)                                                                                          aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg                                                                                                                                          >  1:232882/1‑251 (MQ=255)                                                                                             gcaagcgtccTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCggtgg                                                                                                                                       >  1:537030/9‑251 (MQ=255)                                                                                                   gtccTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                     <  1:385174/213‑1 (MQ=255)                                                                                                        gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGttcga                                                                                                                             >  1:792636/1‑247 (MQ=255)                                                                                                        gatgaCATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGttcga                                                                                                                             >  1:589438/1‑247 (MQ=255)                                                                                                             cATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGc                                                                                                                                                    >  1:222198/1‑222 (MQ=255)                                                                                                                tCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATcc                                                                                                                    <  1:830768/251‑1 (MQ=255)                                                                                                                     aTAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCctgaac                                                                                                               <  1:653584/251‑1 (MQ=255)                                                                                                                                           ttGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTAGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCggagaa                                                                                         >  1:689557/1‑249 (MQ=255)                                                                                                                                                     gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAAc                                                                                <  1:301853/250‑1 (MQ=255)                                                                                                                                                     gAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACg                                                                               >  1:678144/1‑251 (MQ=255)                                                                                                                                                          gaagaaAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                                                                                                                                                                     <  1:28530/160‑1 (MQ=255)                                                                                                                                                                    ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                <  1:603870/251‑2 (MQ=255)                                                                                                                                                                    ttGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                <  1:283630/251‑2 (MQ=255)                                                                                                                                                                      gCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaaga                                                                                                                                                                                   <  1:595820/134‑1 (MQ=255)                                                                                                                                                                              aTTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGcac                                                      <  1:74588/251‑1 (MQ=255)                                                                                                                                                                                 tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGAtc                                                                <  1:456691/238‑2 (MQ=255)                                                                                                                                                                                 tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                   <  1:361764/251‑1 (MQ=255)                                                                                                                                                                                 tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                   <  1:486776/251‑1 (MQ=255)                                                                                                                                                                                 tCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                   <  1:519951/251‑1 (MQ=255)                                                                                                                                                                                      ttcttATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCggaga                                                                  <  1:674734/231‑4 (MQ=255)                                                                                                                                                                                          tATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCAc                                                                            <  1:517075/217‑1 (MQ=255)                                                                                                                                                                                           aTGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAAc                                         <  1:124046/251‑1 (MQ=255)                                                                                                                                                                                             gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaaga                                                                                                                                                                                   >  1:507878/1‑111 (MQ=255)                                                                                                                                                                                             gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                   >  1:323701/1‑239 (MQ=255)                                                                                                                                                                                             gATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACAt                                       >  1:644077/1‑251 (MQ=255)                                                                                                                                                                                                   aacCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAAc                                 <  1:338368/251‑1 (MQ=255)                                                                                                                                                                                                    acCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                   >  1:465414/1‑200 (MQ=255)                                                                                                                                                                                                       caaaaTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTAtt                             <  1:237814/248‑1 (MQ=255)                                                                                                                                                                                                         aaaTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGaagaaaga                                                                                   <  1:67888/194‑1 (MQ=255)                                                                                                                                                                                                           aTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTtata                         <  1:48770/251‑1 (MQ=255)                                                                                                                                                                                                                       cGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGaaaaa             >  1:557630/1‑251 (MQ=255)                                                                                                                                                                                                                          cTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACt          <  1:243630/251‑1 (MQ=255)                                                                                                                                                                                                                              ggaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTgg       >  1:497088/1‑250 (MQ=255)                                                                                                                                                                                                                               gaggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTggc      <  1:151698/250‑2 (MQ=255)                                                                                                                                                                                                                                 ggCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAAc                                                   >  1:75581/1‑203 (MQ=255)                                                                                                                                                                                                                                   caaatATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCGGAGATCCCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGCTccc  >  1:122096/5‑250 (MQ=255)                                                                                                                                                                                                                                    aaatATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCGGTGGTGAAGTTCGACGAAGATCCTGAACCGAAAGAAAAAACGGCGGAGAAGAAAGAAACGCACTAAGCggag                                                                   <  1:520510/181‑3 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGGTGGTGAAGTTTGATGAAGATCCCGAACCGAAGGAAAAAACGGTGGAGGAGAAAGAAACGCACTAAGCGGGGATACCCGGGGCACAACTGTTCGAAACATTGTAACTATTTATATAAAAAGAAAAAACTTGGTTCCC  >  1/730282‑730756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑