Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,378	A→G	intergenic (‑7664/‑56813)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,378	0	A	G	100.0%	219.1 / NA	74	intergenic (‑7664/‑56813)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/40);  total (34/40)

GACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGG  >  1/730137‑730620                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                                                                                                                                                                                                                                            >  1:673041/3‑251 (MQ=255)   cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                                           <  1:441831/250‑1 (MQ=255)   cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                                           <  1:750548/250‑1 (MQ=255)      gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa                                                                                                                                                                                                                                        <  1:806194/250‑1 (MQ=255)         ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                                                                                                                                                                                                                                          <  1:537666/245‑1 (MQ=255)         ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa                                                                                                                                                                                                                                    >  1:220851/1‑251 (MQ=255)           ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                                                                                  >  1:896113/1‑250 (MQ=255)           ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                                                                                  >  1:216956/1‑250 (MQ=255)             tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat                                                                                                                                                                                                                                <  1:442306/251‑4 (MQ=255)                  cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                                                                           <  1:298862/249‑1 (MQ=255)                                 tttAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggttggcaaa                                                                                                                                                                                                            >  1:739232/1‑243 (MQ=255)                                               gCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                                                                              >  1:779751/1‑251 (MQ=255)                                                ccccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                                                                              <  1:270092/250‑1 (MQ=255)                                                  ccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGt                                                                                                                                                                                             >  1:242533/1‑249 (MQ=255)                                                    tttCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                                                                         >  1:567562/1‑251 (MQ=255)                                                      tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                                                                           <  1:593913/212‑1 (MQ=255)                                                       cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                                                                      <  1:848411/249‑1 (MQ=255)                                                       cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                                                                      >  1:701517/3‑251 (MQ=255)                                                          tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                                                                                   <  1:785990/251‑1 (MQ=255)                                                          tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                                                                                   <  1:679235/251‑1 (MQ=255)                                                           gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                                                                       <  1:284703/214‑1 (MQ=255)                                                           gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                                                                         <  1:770914/244‑1 (MQ=255)                                                              cccGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggt                                                                                                                                                                                                                   <  1:58294/215‑2 (MQ=255)                                                                           cAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                                           >  1:268539/1‑178 (MQ=255)                                                                                                   tGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                                                                          <  1:332150/251‑1 (MQ=255)                                                                                                         cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAAc                                                                                                                                     <  1:152514/250‑1 (MQ=255)                                                                                                           cAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACgg                                                                                                                                   >  1:516656/1‑250 (MQ=255)                                                                                                                      accggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                                                                                                       <  1:26268/240‑1 (MQ=255)                                                                                                                       ccggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                                                                       <  1:515173/144‑1 (MQ=255)                                                                                                                              ggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTAATGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                                                                               >  1:122563/4‑249 (MQ=255)                                                                                                                               gtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                                                                               <  1:261971/248‑3 (MQ=255)                                                                                                                                    gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaatctgac                                                                                                          <  1:82166/250‑8 (MQ=255)                                                                                                                                    gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATgac                                                                                                          <  1:272769/250‑3 (MQ=255)                                                                                                                                                  tttCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTaaaaa                                                                                           >  1:599641/1‑251 (MQ=255)                                                                                                                                                    tcttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTgaag                                                                                                     <  1:809826/239‑1 (MQ=255)                                                                                                                                                     cttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAgg                                                                                        >  1:164548/1‑251 (MQ=255)                                                                                                                                                      ttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                                       <  1:50446/251‑1 (MQ=255)                                                                                                                                                           accGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATaac                                                                                                                                                    >  1:270628/2‑185 (MQ=255)                                                                                                                                                            ccGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTa                                                                                 >  1:848823/1‑251 (MQ=255)                                                                                                                                                                  gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACt                                                                                                                           <  1:660204/203‑1 (MQ=255)                                                                                                                                                                  gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                                            <  1:497927/250‑1 (MQ=255)                                                                                                                                                                   cGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcgcg                                                                          >  1:716448/1‑251 (MQ=255)                                                                                                                                                                        gtcttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTgg                                                                     >  1:490986/3‑251 (MQ=255)                                                                                                                                                                          cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                   <  1:198214/251‑1 (MQ=255)                                                                                                                                                                          cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                   <  1:394941/251‑1 (MQ=255)                                                                                                                                                                          cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGAGGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                   <  1:435084/251‑1 (MQ=255)                                                                                                                                                                               ccGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTaa                                                                                <  1:543942/233‑1 (MQ=255)                                                                                                                                                                                  cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                                           >  1:474711/1‑251 (MQ=255)                                                                                                                                                                                  cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                                           >  1:243985/1‑251 (MQ=255)                                                                                                                                                                                    aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAGTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                                       <  1:118271/221‑1 (MQ=255)                                                                                                                                                                                    aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                                                                          >  1:87641/1‑170 (MQ=255)                                                                                                                                                                                    aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTcc                                                         <  1:25708/251‑1 (MQ=255)                                                                                                                                                                                      tCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGa                                                       <  1:730850/251‑1 (MQ=255)                                                                                                                                                                                                   aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGccta                                                             >  1:115330/5‑229 (MQ=255)                                                                                                                                                                                                   aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaaga                                           <  1:674475/246‑1 (MQ=255)                                                                                                                                                                                                    aagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaagaag                                         >  1:766491/4‑251 (MQ=255)                                                                                                                                                                                                          gCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAatat                                                                                                             >  1:872792/1‑177 (MQ=255)                                                                                                                                                                                                           cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt                                  <  1:697933/251‑7 (MQ=255)                                                                                                                                                                                                           cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt                                  <  1:280371/251‑7 (MQ=255)                                                                                                                                                                                                               gaacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAACAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                                            >  1:516088/6‑205 (MQ=255)                                                                                                                                                                                                               gaacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                                                                                                       <  1:242297/157‑1 (MQ=255)                                                                                                                                                                                                                aacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc                             <  1:114500/247‑1 (MQ=255)                                                                                                                                                                                                                          tCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAgg                         >  1:651044/1‑245 (MQ=255)                                                                                                                                                                                                                           ccTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                                                   >  1:6763/1‑202 (MQ=255)                                                                                                                                                                                                                            cTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTc                                                          <  1:155664/210‑1 (MQ=255)                                                                                                                                                                                                                                aTTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGc                                                <  1:674247/216‑1 (MQ=255)                                                                                                                                                                                                                                       gCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATa                                                                                                                                                      >  1:95856/1‑107 (MQ=255)                                                                                                                                                                                                                                       gCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAACATAAAACGGCATCGACTAAGGAGGCAAATACGACTGAAGAAGCTAAAAAAGGTTGGCTAAAACGCGCTTgggcagcc                                                               >  1:700849/1‑189 (MQ=255)                                                                                                                                                                                                                                        ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc     <  1:580848/251‑3 (MQ=255)                                                                                                                                                                                                                                        ccTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtg      >  1:728463/1‑249 (MQ=255)                                                                                                                                                                                                                                         cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc     >  1:765256/1‑248 (MQ=255)                                                                                                                                                                                                                                         cTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGtgc     >  1:746828/1‑248 (MQ=255)                                                                                                                                                                                                                                           aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg  >  1:837236/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAGGCACTTGCCGGGGCTGCGTGCCgg  >  1:232882/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    GACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGGCACTTGCCGAGGCTGCGTACCGG  >  1/730137‑730620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑