Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,364	A→G	intergenic (‑7650/‑56827)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,364	0	A	G	100.0%	224.4 / NA	76	intergenic (‑7650/‑56827)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/46);  total (30/46)

TGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGG  >  1/730118‑730597                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           tgtccGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGc                                                                                                                                                                                                                                        <  1:410682/248‑1 (MQ=255)        taaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGc                                                                                                                                                                                                                                 <  1:664681/249‑1 (MQ=255)         aaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCa                                                                                                                                                                                                                                <  1:551660/250‑1 (MQ=255)          aCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaa                                                                                                                                                                                                                               <  1:475604/251‑1 (MQ=255)               gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                                                                                           >  1:46631/8‑246 (MQ=255)               gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                                                                                           >  1:253026/8‑246 (MQ=255)                cacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgtcc                                                                                                                                                                                                                         <  1:890464/245‑7 (MQ=255)                    ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                                                                                                                                                                                                                     >  1:673041/3‑251 (MQ=255)                      cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                    <  1:441831/250‑1 (MQ=255)                      cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                    <  1:750548/250‑1 (MQ=255)                         gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa                                                                                                                                                                                                                 <  1:806194/250‑1 (MQ=255)                            ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                                                                                                                                                                                                                   <  1:537666/245‑1 (MQ=255)                            ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa                                                                                                                                                                                                             >  1:220851/1‑251 (MQ=255)                              ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                                                           >  1:896113/1‑250 (MQ=255)                              ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                                                           >  1:216956/1‑250 (MQ=255)                                tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat                                                                                                                                                                                                         <  1:442306/251‑4 (MQ=255)                                     cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                                                    <  1:298862/249‑1 (MQ=255)                                      gggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                                                                                            >  1:463500/2‑223 (MQ=255)                                                    tttAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggttggcaaa                                                                                                                                                                                     >  1:739232/1‑243 (MQ=255)                                                                  gCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                                                       >  1:779751/1‑251 (MQ=255)                                                                   ccccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                                                       <  1:270092/250‑1 (MQ=255)                                                                     ccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGt                                                                                                                                                                      >  1:242533/1‑249 (MQ=255)                                                                       tttCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                                                  >  1:567562/1‑251 (MQ=255)                                                                         tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                                                    <  1:593913/212‑1 (MQ=255)                                                                          cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                                                                                            <  1:745488/188‑4 (MQ=255)                                                                          cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                                               <  1:848411/249‑1 (MQ=255)                                                                          cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                                               >  1:701517/3‑251 (MQ=255)                                                                             tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                                                            <  1:679235/251‑1 (MQ=255)                                                                             tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                                                            <  1:785990/251‑1 (MQ=255)                                                                              gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                                                <  1:284703/214‑1 (MQ=255)                                                                              gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                                                  <  1:770914/244‑1 (MQ=255)                                                                                 cccGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggt                                                                                                                                                                                            <  1:58294/215‑2 (MQ=255)                                                                                              cAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                                                    >  1:268539/1‑178 (MQ=255)                                                                                                                      tGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                                                   <  1:332150/251‑1 (MQ=255)                                                                                                                            cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaag                                                                                                                                                                                                                              <  1:629123/138‑2 (MQ=255)                                                                                                                            cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAAc                                                                                                              <  1:152514/250‑1 (MQ=255)                                                                                                                              cAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACgg                                                                                                            >  1:516656/1‑250 (MQ=255)                                                                                                                                         accggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                                                                                <  1:26268/240‑1 (MQ=255)                                                                                                                                          ccggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                                                <  1:515173/144‑1 (MQ=255)                                                                                                                                                 ggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTAATGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                                                        >  1:122563/4‑249 (MQ=255)                                                                                                                                                  gtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                                                        <  1:261971/248‑3 (MQ=255)                                                                                                                                                       gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaatctgac                                                                                   <  1:82166/250‑8 (MQ=255)                                                                                                                                                       gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATgac                                                                                   <  1:272769/250‑3 (MQ=255)                                                                                                                                                                     tttCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTaaaaa                                                                    >  1:599641/1‑251 (MQ=255)                                                                                                                                                                       tcttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTgaag                                                                              <  1:809826/239‑1 (MQ=255)                                                                                                                                                                        cttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAgg                                                                 >  1:164548/1‑251 (MQ=255)                                                                                                                                                                         ttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                <  1:50446/251‑1 (MQ=255)                                                                                                                                                                              accGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATaac                                                                                                                             >  1:270628/2‑185 (MQ=255)                                                                                                                                                                               ccGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTa                                                          >  1:848823/1‑251 (MQ=255)                                                                                                                                                                                     gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACt                                                                                                    <  1:660204/203‑1 (MQ=255)                                                                                                                                                                                     gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                     <  1:497927/250‑1 (MQ=255)                                                                                                                                                                                      cGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcgcg                                                   >  1:716448/1‑251 (MQ=255)                                                                                                                                                                                           gtcttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTgg                                              >  1:490986/3‑251 (MQ=255)                                                                                                                                                                                             cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                            <  1:394941/251‑1 (MQ=255)                                                                                                                                                                                             cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                            <  1:198214/251‑1 (MQ=255)                                                                                                                                                                                             cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGAGGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                            <  1:435084/251‑1 (MQ=255)                                                                                                                                                                                                  ccGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTaa                                                         <  1:543942/233‑1 (MQ=255)                                                                                                                                                                                                     cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                    >  1:474711/1‑251 (MQ=255)                                                                                                                                                                                                     cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt                                    >  1:243985/1‑251 (MQ=255)                                                                                                                                                                                                       aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAGTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                                                <  1:118271/221‑1 (MQ=255)                                                                                                                                                                                                       aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                                                   >  1:87641/1‑170 (MQ=255)                                                                                                                                                                                                       aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTcc                                  <  1:25708/251‑1 (MQ=255)                                                                                                                                                                                                         tCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGa                                <  1:730850/251‑1 (MQ=255)                                                                                                                                                                                                                      aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGccta                                      >  1:115330/5‑229 (MQ=255)                                                                                                                                                                                                                      aaagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaaga                    <  1:674475/246‑1 (MQ=255)                                                                                                                                                                                                                       aagtAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAaagaag                  >  1:766491/4‑251 (MQ=255)                                                                                                                                                                                                                             gCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAatat                                                                                      >  1:872792/1‑177 (MQ=255)                                                                                                                                                                                                                              cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt           <  1:697933/251‑7 (MQ=255)                                                                                                                                                                                                                              cGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGccggtgt           <  1:280371/251‑7 (MQ=255)                                                                                                                                                                                                                                  gaacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAACAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                                                     >  1:516088/6‑205 (MQ=255)                                                                                                                                                                                                                                  gaacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                                                                                <  1:242297/157‑1 (MQ=255)                                                                                                                                                                                                                                   aacaggATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATc      <  1:114500/247‑1 (MQ=255)                                                                                                                                                                                                                                             tCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGCAAAGAAGCCGGTGTGGATCAAgg  >  1:651044/1‑245 (MQ=255)                                                                                                                                                                                                                                              ccTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa                                            >  1:6763/1‑202 (MQ=255)                                                                                                                                                                                                                                               cTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTc                                   <  1:155664/210‑1 (MQ=255)                                                                                                                                                                                                                                                   aTTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGATTTCTGc                         <  1:674247/216‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           TGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGATTTCTGCAAAGAAGCAGGCGTGGATCAAGG  >  1/730118‑730597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑