Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,334	T→G	intergenic (‑7620/‑56857)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,334	0	T	G	100.0%	225.3 / NA	75	intergenic (‑7620/‑56857)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/43);  total (32/43)

GCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGA  >  1/730092‑730567                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           gCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCggg                                                                                                                                                                                                                                    >  1:323976/1‑251 (MQ=255)     cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaac                                                                                                                                                                                                                                <  1:205980/251‑3 (MQ=255)     cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                                                 >  1:548670/1‑249 (MQ=255)     cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                                                 >  1:858142/1‑249 (MQ=255)     cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                                                 >  1:23021/1‑249 (MQ=255)           ttCCGTGGTGGCGCTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGAtt                                                                                                                                                                                                                          >  1:728512/1‑251 (MQ=255)               gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                                                                                                                                                       >  1:214156/4‑250 (MQ=255)               gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                                                                                                                                                       >  1:457608/4‑250 (MQ=255)                tggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCt                                                                                                                                                                                                                     <  1:616559/249‑1 (MQ=255)                  gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGc                                                                                                                                                                                                                                       <  1:752862/231‑1 (MQ=255)                  gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCttt                                                                                                                                                                                                                   >  1:332777/1‑251 (MQ=255)                           tgtccGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGc                                                                                                                                                                                                          <  1:410682/248‑1 (MQ=255)                                  taaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGc                                                                                                                                                                                                   <  1:664681/249‑1 (MQ=255)                                   aaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCa                                                                                                                                                                                                  <  1:551660/250‑1 (MQ=255)                                    aCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaa                                                                                                                                                                                                 <  1:475604/251‑1 (MQ=255)                                         gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                                                             >  1:46631/8‑246 (MQ=255)                                         gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                                                             >  1:253026/8‑246 (MQ=255)                                          cacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgtcc                                                                                                                                                                                           <  1:890464/245‑7 (MQ=255)                                              ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                                                                                                                                                                                       >  1:673041/3‑251 (MQ=255)                                                cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                      <  1:441831/250‑1 (MQ=255)                                                cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                      <  1:750548/250‑1 (MQ=255)                                                   gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa                                                                                                                                                                                   <  1:806194/250‑1 (MQ=255)                                                      ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                                                                                                                                                                                     <  1:537666/245‑1 (MQ=255)                                                      ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa                                                                                                                                                                               >  1:220851/1‑251 (MQ=255)                                                        ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                             >  1:896113/1‑250 (MQ=255)                                                        ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                                                             >  1:216956/1‑250 (MQ=255)                                                          tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat                                                                                                                                                                           <  1:442306/251‑4 (MQ=255)                                                               cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                      <  1:298862/249‑1 (MQ=255)                                                                gggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                                                              >  1:463500/2‑223 (MQ=255)                                                                              tttAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggttggcaaa                                                                                                                                                       >  1:739232/1‑243 (MQ=255)                                                                                            gCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                         >  1:779751/1‑251 (MQ=255)                                                                                             ccccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                                                         <  1:270092/250‑1 (MQ=255)                                                                                               ccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGt                                                                                                                                        >  1:242533/1‑249 (MQ=255)                                                                                                 tttCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                    >  1:567562/1‑251 (MQ=255)                                                                                                   tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaacaggat                                                                                                                                                                                                                           <  1:229641/159‑8 (MQ=255)                                                                                                   tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                                                      <  1:593913/212‑1 (MQ=255)                                                                                                    cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                                                              <  1:745488/188‑4 (MQ=255)                                                                                                    cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                 <  1:848411/249‑1 (MQ=255)                                                                                                    cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                                                 >  1:701517/3‑251 (MQ=255)                                                                                                       tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                              <  1:785990/251‑1 (MQ=255)                                                                                                       tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                                                              <  1:679235/251‑1 (MQ=255)                                                                                                        gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                  <  1:284703/214‑1 (MQ=255)                                                                                                        gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                                                    <  1:770914/244‑1 (MQ=255)                                                                                                           cccGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggt                                                                                                                                                              <  1:58294/215‑2 (MQ=255)                                                                                                                        cAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                                                      >  1:268539/1‑178 (MQ=255)                                                                                                                                                tGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                     <  1:332150/251‑1 (MQ=255)                                                                                                                                                      cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaag                                                                                                                                                                                                <  1:629123/138‑2 (MQ=255)                                                                                                                                                      cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAAc                                                                                <  1:152514/250‑1 (MQ=255)                                                                                                                                                        cAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACgg                                                                              >  1:516656/1‑250 (MQ=255)                                                                                                                                                                   accggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                                                  <  1:26268/240‑1 (MQ=255)                                                                                                                                                                    ccggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                                                  <  1:515173/144‑1 (MQ=255)                                                                                                                                                                           ggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTAATGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                          >  1:122563/4‑249 (MQ=255)                                                                                                                                                                            gtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                                                          <  1:261971/248‑3 (MQ=255)                                                                                                                                                                                 gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaatctgac                                                     <  1:82166/250‑8 (MQ=255)                                                                                                                                                                                 gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATgac                                                     <  1:272769/250‑3 (MQ=255)                                                                                                                                                                                               tttCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTaaaaa                                      >  1:599641/1‑251 (MQ=255)                                                                                                                                                                                                 tcttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTgaag                                                <  1:809826/239‑1 (MQ=255)                                                                                                                                                                                                  cttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAgg                                   >  1:164548/1‑251 (MQ=255)                                                                                                                                                                                                   ttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                  <  1:50446/251‑1 (MQ=255)                                                                                                                                                                                                        accGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATaac                                                                                               >  1:270628/2‑185 (MQ=255)                                                                                                                                                                                                         ccGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTa                            >  1:848823/1‑251 (MQ=255)                                                                                                                                                                                                               gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACt                                                                      <  1:660204/203‑1 (MQ=255)                                                                                                                                                                                                               gCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcg                       <  1:497927/250‑1 (MQ=255)                                                                                                                                                                                                                cGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAAcgcg                     >  1:716448/1‑251 (MQ=255)                                                                                                                                                                                                                     gtcttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTgg                >  1:490986/3‑251 (MQ=255)                                                                                                                                                                                                                       cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa              <  1:198214/251‑1 (MQ=255)                                                                                                                                                                                                                       cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa              <  1:394941/251‑1 (MQ=255)                                                                                                                                                                                                                       cttgccCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGAGGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGa              <  1:435084/251‑1 (MQ=255)                                                                                                                                                                                                                            ccGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTaa                           <  1:543942/233‑1 (MQ=255)                                                                                                                                                                                                                               cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt      >  1:243985/1‑251 (MQ=255)                                                                                                                                                                                                                               cTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTAtt      >  1:474711/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAGTAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt                                  <  1:118271/221‑1 (MQ=255)                                                                                                                                                                                                                                 aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                                                     >  1:87641/1‑170 (MQ=255)                                                                                                                                                                                                                                 aTTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTcc    <  1:25708/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCCTATTCCGa  <  1:730850/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           GCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGTTGGTTAAAACGCGCTTGGGATGCGTATTCCGA  >  1/730092‑730567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑