Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	730,162	G→A	intergenic (‑7448/‑57029)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	730,162	0	G	A	100.0%	205.7 / NA	69	intergenic (‑7448/‑57029)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/34);  total (35/34)

ACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAACGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTGCTGGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAA  >  1/729922‑730404                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   gcccAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTa                                                                                                                                                                                                                                          >  1:635730/2‑251 (MQ=255)         ggCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAAt                                                                                                                                                                                                                                  >  1:168332/1‑251 (MQ=255)              cagcGAGGGCTGGGGCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGc                                                                                                                                                                                                                              >  1:819576/1‑250 (MQ=255)                 cgagggcTGGGGCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGcccc                                                                                                                                                                                                                           <  1:465959/244‑1 (MQ=255)                     ggcTGGGGCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTc                                                                                                                                                                                                                       <  1:447361/248‑1 (MQ=255)                          gggCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGc                                                                                                                                                                                                                  <  1:704049/250‑1 (MQ=255)                          gggCAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCa                                                                                                                                                                                                                 >  1:476885/1‑251 (MQ=255)                             cAACGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGTAcc                                                                                                                                                                                                               <  1:709743/250‑1 (MQ=255)                               aCGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTg                                                                                                                                                                                                                   <  1:881949/244‑1 (MQ=255)                               aCGTTTAATCGAAC‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCg                                                                                                                                                                                                             >  1:810302/1‑250 (MQ=255)                                               gattAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTg                                                                                                                                                                                             >  1:140263/3‑251 (MQ=255)                                               gattAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTg                                                                                                                                                                                             >  1:559844/3‑251 (MQ=255)                                               gattAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTg                                                                                                                                                                                             >  1:807808/3‑251 (MQ=255)                                                 ttAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGc                                                                                                                                                                                                                  >  1:77605/1‑228 (MQ=255)                                                 ttAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTgcc                                                                                                                                                                                           <  1:422809/251‑3 (MQ=255)                                                     acaacaacGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGaa                                                                                                                                                                                       >  1:394151/1‑251 (MQ=255)                                                                     gATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGt                                                                                                                                                                        <  1:28006/250‑1 (MQ=255)                                                                              tccGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGa                                                                                                                                                              <  1:25636/251‑1 (MQ=255)                                                                                cGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGaag                                                                                                                                                            <  1:8584/251‑2 (MQ=255)                                                                                     gTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCAcc                                                                                                                                                                                                               >  1:497447/1‑195 (MQ=255)                                                                                     gTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGa                                                                                                                                                        >  1:622352/1‑250 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCgg                                                                                                                                                                                         <  1:780018/205‑1 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGaagc                                                                                                                                                           <  1:571006/235‑3 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggccaggtttg                                                                                                                                           >  1:855603/1‑238 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                           >  1:38965/1‑238 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                           >  1:766329/1‑238 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                           >  1:497213/1‑238 (MQ=255)                                                                                                 cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                           >  1:271732/1‑238 (MQ=255)                                                                                                   cccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttgg                                                                                                                                          >  1:748746/1‑236 (MQ=255)                                                                                                    ccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCg                                                                                                                                        <  1:41675/251‑1 (MQ=255)                                                                                                                       gCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATttcttc                                                                                                                      <  1:383121/250‑1 (MQ=255)                                                                                                                          aaaGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCtac                                                                                                                  <  1:35151/251‑3 (MQ=255)                                                                                                                              tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                <  1:672984/249‑1 (MQ=255)                                                                                                                              tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                <  1:639961/249‑1 (MQ=255)                                                                                                                              tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCCAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                <  1:429372/249‑1 (MQ=255)                                                                                                                                  gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                          >  1:139986/1‑251 (MQ=255)                                                                                                                                  gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                          >  1:550499/1‑251 (MQ=255)                                                                                                                                                                    tgcgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaag                                                                        <  1:88031/247‑2 (MQ=255)                                                                                                                                                                      cgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                      <  1:458958/249‑4 (MQ=255)                                                                                                                                                                            gCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCggg                                                                >  1:323976/1‑251 (MQ=255)                                                                                                                                                                                cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaac                                                            <  1:205980/251‑3 (MQ=255)                                                                                                                                                                                cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                             >  1:23021/1‑249 (MQ=255)                                                                                                                                                                                cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                             >  1:548670/1‑249 (MQ=255)                                                                                                                                                                                cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                             >  1:858142/1‑249 (MQ=255)                                                                                                                                                                                      ttCCGTGGTGGCGCTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGAtt                                                      >  1:728512/1‑251 (MQ=255)                                                                                                                                                                                          gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                   >  1:214156/4‑250 (MQ=255)                                                                                                                                                                                          gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                   >  1:457608/4‑250 (MQ=255)                                                                                                                                                                                           tggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCt                                                 <  1:616559/249‑1 (MQ=255)                                                                                                                                                                                             gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGc                                                                   <  1:752862/231‑1 (MQ=255)                                                                                                                                                                                             gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCttt                                               >  1:332777/1‑251 (MQ=255)                                                                                                                                                                                                      tgtccGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGc                                      <  1:410682/248‑1 (MQ=255)                                                                                                                                                                                                             taaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGc                               <  1:664681/249‑1 (MQ=255)                                                                                                                                                                                                              aaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCa                              <  1:551660/250‑1 (MQ=255)                                                                                                                                                                                                               aCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaa                             <  1:475604/251‑1 (MQ=255)                                                                                                                                                                                                                    gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                         >  1:253026/8‑246 (MQ=255)                                                                                                                                                                                                                    gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                         >  1:46631/8‑246 (MQ=255)                                                                                                                                                                                                                     cacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgtcc                       <  1:890464/245‑7 (MQ=255)                                                                                                                                                                                                                         ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                   >  1:673041/3‑251 (MQ=255)                                                                                                                                                                                                                           cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                  <  1:441831/250‑1 (MQ=255)                                                                                                                                                                                                                           cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                  <  1:750548/250‑1 (MQ=255)                                                                                                                                                                                                                              gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa               <  1:806194/250‑1 (MQ=255)                                                                                                                                                                                                                                 ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                 <  1:537666/245‑1 (MQ=255)                                                                                                                                                                                                                                 ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa           >  1:220851/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa         >  1:216956/1‑250 (MQ=255)                                                                                                                                                                                                                                   ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa         >  1:896113/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                      <  1:788098/189‑4 (MQ=255)                                                                                                                                                                                                                                     tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat       <  1:442306/251‑4 (MQ=255)                                                                                                                                                                                                                                          cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa  <  1:298862/249‑1 (MQ=255)                                                                                                                                                                                                                                           gggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                          >  1:463500/2‑223 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAACGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTGCTGGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAA  >  1/729922‑730404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑