| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 729,808 | G→A | intergenic (‑7094/‑57383) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 729,808 | 0 | G | A | 98.6% | 196.5 / NA | 70 | intergenic (‑7094/‑57383) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (33/36); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCTGTGCCTGAGGGCGAATTTGGTGACGACGCATTAATTCGTAAAATTCGGCGCGGACAACGTCGTCCACGTGGTAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCACTGCGCATTTCCCGTATCGCCGTGCAACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAA‑CGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTG > 1/729590‑730027 | attTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctgcgcggcaatttgg < 1:130677/250‑18 (MQ=255)attTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctgcgcggcaatttgg < 1:28453/250‑18 (MQ=255) aagaaCGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtg < 1:798858/251‑1 (MQ=255) aacggATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtgttg < 1:809807/247‑1 (MQ=255) cggATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTtgtg < 1:390128/249‑1 (MQ=255) aatcgGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctg > 1:114989/1‑196 (MQ=255) cgGTTTATGGCTTGCTGACTTTGGCGCATTACCTCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGGCGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCg < 1:506287/251‑1 (MQ=255) ggTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtc > 1:739850/1‑191 (MQ=255) gTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc < 1:882176/244‑1 (MQ=255) tATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa < 1:733341/251‑1 (MQ=255) gCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtg > 1:390954/1‑208 (MQ=255) ttacagcaCCTCGCCGTTGGATTTACGACGATTATTGGATGCACCGAGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGAGTGTGGGCGGAGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGGAAATTGGGTCCTTTGCGCATTTcc > 1:210964/8‑250 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGAc < 1:97440/158‑1 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTtgtg < 1:701921/198‑1 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc > 1:728742/1‑218 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc > 1:209876/1‑250 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc > 1:698893/1‑250 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc < 1:582402/250‑1 (MQ=255) ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc > 1:51847/1‑250 (MQ=255) aCCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa > 1:632915/1‑226 (MQ=255) tGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGccc < 1:158901/251‑1 (MQ=255) attattGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCagcagc < 1:69320/251‑1 (MQ=255) attGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgag > 1:232337/1‑249 (MQ=255) attGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgag > 1:483325/1‑249 (MQ=255) ttGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGcagcag > 1:789812/1‑246 (MQ=255) ttGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgagg > 1:781539/1‑248 (MQ=255) caccgcGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTgggg < 1:759887/249‑1 (MQ=255) gcgACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa < 1:283615/183‑1 (MQ=255) caaTTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt < 1:143521/251‑1 (MQ=255) attttattttGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGTCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGGATCGCCGTGc > 1:615681/1‑209 (MQ=255) attttattttGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCg < 1:445071/251‑1 (MQ=255) attttgcacaaacgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGAAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt < 1:9417/220‑1 (MQ=255) aacgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaac < 1:144890/237‑1 (MQ=255) cgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt > 1:790218/13‑232 (MQ=255) cgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa < 1:585054/239‑1 (MQ=255) ggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGt < 1:91252/242‑1 (MQ=255) ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc > 1:701442/2‑136 (MQ=255) ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTggg > 1:308059/2‑250 (MQ=255) ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTggg > 1:596187/2‑250 (MQ=255) gttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGAtt > 1:386119/1‑251 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTa > 1:833883/1‑162 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑cagattaaac < 1:539036/225‑10 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa < 1:471552/230‑1 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGt > 1:297852/1‑233 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGAttttctttc > 1:892622/1‑250 (MQ=255) gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGGGGAttttctttc > 1:30271/1‑250 (MQ=255) gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTTCCGTATCGCCGTGCAGCCCAACTGGCCGCAGCGAGGGCTGGGGCCACGTTTAATCGAACCGA‑‑TTAAACAACAACCTGCGGTGGATTTTCTTTCCGt > 1:16077/1‑251 (MQ=255) gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa < 1:437368/228‑1 (MQ=255) gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACg > 1:323252/1‑230 (MQ=255) gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGt > 1:598662/1‑251 (MQ=255) tgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGATCTTTGCGCATTTCCCGCATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCCGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGACTTTCTTTCCGt > 1:85052/1‑250 (MQ=255) gtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGc > 1:830742/1‑162 (MQ=255) gaaggaggatttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTaca > 1:338311/22‑250 (MQ=255) gatttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGGTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaa > 1:770770/15‑250 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGc < 1:147719/133‑1 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:578174/238‑3 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:217141/238‑3 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:149617/238‑3 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:836462/238‑3 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:543915/238‑3 (MQ=255) atttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGGTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:446276/238‑3 (MQ=255) ttgaggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAactg < 1:305880/240‑1 (MQ=255) ggatgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑cagattaaac > 1:487161/8‑185 (MQ=255) tgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgagggc < 1:227295/157‑6 (MQ=255) tgatgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCg < 1:390200/214‑1 (MQ=255) atgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAAt < 1:473155/100‑1 (MQ=255) atgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑cagattaaaca > 1:325522/3‑180 (MQ=255) atgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACAcccc < 1:848980/233‑1 (MQ=255) atgCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac > 1:366912/3‑238 (MQ=255) gtcaaaTTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGaac < 1:143746/225‑3 (MQ=255) | ATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCTGTGCCTGAGGGCGAATTTGGTGACGACGCATTAATTCGTAAAATTCGGCGCGGACAACGTCGTCCACGTGGTAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCACTGCGCATTTCCCGTATCGCCGTGCAACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAA‑CGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTG > 1/729590‑730027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |