Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	729,388	C→T	intergenic (‑6674/‑57803)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	729,388	0	C	T	100.0%	185.5 / NA	65	intergenic (‑6674/‑57803)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/31);  total (34/31)

CGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGATGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGACGCAACCGCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGA  >  1/729174‑729627                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                tgAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTc                                                                                                                                                                                                             <  1:234747/250‑1 (MQ=255)  gAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCa                                                                                                                                                                                                            >  1:856567/1‑251 (MQ=255)             cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                                                                                                                                                                                  <  1:348820/250‑10 (MQ=255)             cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                                                                                                                                                                                  <  1:803534/250‑10 (MQ=255)               gTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccga                                                                                                                                                                                               >  1:265432/1‑251 (MQ=255)                 caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGTTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                                                                                                                                                              >  1:872472/5‑250 (MQ=255)                 caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                                                                                                                                                              >  1:408353/5‑250 (MQ=255)                 caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                                                                                                                                                              >  1:196735/5‑250 (MQ=255)                 caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                                                                                                                                                              >  1:777083/5‑250 (MQ=255)                   ataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGGTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGCTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgaccg                                                                                                                                                                                            >  1:879924/3‑250 (MQ=255)                                           cggAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACttttgttcagcgccgtcaatag                                                                                                                                                                   <  1:401924/249‑20 (MQ=255)                                                    cgtttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGccc                                                                                                                                                              <  1:192737/236‑1 (MQ=255)                                                      tttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACg                                                                                                                                                        <  1:513996/242‑1 (MQ=255)                                                      tttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACg                                                                                                                                                        <  1:552992/242‑1 (MQ=255)                                                        tatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTACACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGtt                                                                                                                                                      >  1:580939/8‑251 (MQ=255)                                                               ccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAAt                                                                                                                                                                        <  1:440071/226‑1 (MQ=255)                                                               ccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCa                                                                                                                                                  <  1:260459/248‑1 (MQ=255)                                                                      aaCTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGAc                                                                                                                                        <  1:210795/251‑1 (MQ=255)                                                                       aCTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAAt                                                                                                                                                                        <  1:267978/218‑1 (MQ=255)                                                                          gcgtcgaaaGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACaaa                                                                                                                                     >  1:230114/7‑250 (MQ=255)                                                                           cgtcgaaaGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAAc                                                                                                                                    <  1:688801/245‑1 (MQ=255)                                                                                   gattGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAgcg                                                                                                                             >  1:766206/3‑249 (MQ=255)                                                                                        gTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTAt                                                                                                                       <  1:102530/250‑1 (MQ=255)                                                                                           aaaaTCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATtgac                                                                                                                   <  1:86345/251‑2 (MQ=255)                                                                                           aaaaTCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATtga                                                                                                                    <  1:443041/250‑1 (MQ=255)                                                                                                       aaTTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAAt                                                                                                                                                                        <  1:819218/186‑1 (MQ=255)                                                                                                       aaTTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAAttgttgttg                                                                                                       <  1:772507/251‑1 (MQ=255)                                                                                                               gatttatGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACttttgttc                                                                                                                                                                                 <  1:180766/163‑6 (MQ=255)                                                                                                               gatttatGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAAt                                                                                                               <  1:6438/229‑1 (MQ=255)                                                                                                                    atGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                                                                                                                                                                                  >  1:669801/2‑138 (MQ=255)                                                                                                                      ggTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGcc                                                                                                                                                               <  1:655524/180‑1 (MQ=255)                                                                                                                      ggTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGAt                                                                                        >  1:523360/1‑251 (MQ=255)                                                                                                                       gTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGccc                                                                                                                                                              >  1:266618/1‑180 (MQ=255)                                                                                                                                 gatgAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCa                                                                             >  1:699861/4‑251 (MQ=255)                                                                                                                                     aaGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGATCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAAcc                                                                          <  1:617817/250‑1 (MQ=255)                                                                                                                                          gcgATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTtgtg                                                                                                     <  1:218698/218‑1 (MQ=255)                                                                                                                                             atgatCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatga                                                                 <  1:144935/251‑4 (MQ=255)                                                                                                                                               gatcccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCa                                                                                                                                                  >  1:714248/5‑168 (MQ=255)                                                                                                                                                atcccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatgacg                                                               >  1:481754/4‑245 (MQ=255)                                                                                                                                                atcccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatga                                                                 >  1:227623/4‑245 (MQ=255)                                                                                                                                                 tcccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAAttgttgttg                                                                                                       >  1:778456/3‑209 (MQ=255)                                                                                                                                                 gcccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatgacg                                                               >  1:538881/3‑244 (MQ=255)                                                                                                                                                   ccGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAAttgttgt                                                                                                         >  1:207128/1‑205 (MQ=255)                                                                                                                                                     gTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatgacgctaac                                                          >  1:574376/1‑240 (MQ=255)                                                                                                                                                      ttGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatgacgctaacc                                                         <  1:386367/250‑12 (MQ=255)                                                                                                                                                       gggATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATtgac                                                                                                                   <  1:592032/190‑2 (MQ=255)                                                                                                                                                         gATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatga                                                                 <  1:17517/239‑4 (MQ=255)                                                                                                                                                          aTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGc                                                     >  1:462804/1‑250 (MQ=255)                                                                                                                                                             cGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGccgaa                                                 >  1:595812/1‑248 (MQ=255)                                                                                                                                                             cGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGccgaa                                                 >  1:279059/1‑248 (MQ=255)                                                                                                                                                                              gccgtcTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTa                                                                    >  1:840581/6‑215 (MQ=255)                                                                                                                                                                                   cTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACaaag                           >  1:801365/1‑251 (MQ=255)                                                                                                                                                                                           gcaTTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGAtt                   >  1:429962/3‑251 (MQ=255)                                                                                                                                                                                              ttGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGTACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                  <  1:447481/249‑1 (MQ=255)                                                                                                                                                                                              ttGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                  <  1:535326/249‑1 (MQ=255)                                                                                                                                                                                                gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTa                                                                    >  1:887053/1‑197 (MQ=255)                                                                                                                                                                                                gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc               >  1:328430/1‑250 (MQ=255)                                                                                                                                                                                                gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc               >  1:713065/1‑250 (MQ=255)                                                                                                                                                                                                gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc               >  1:408326/1‑250 (MQ=255)                                                                                                                                                                                                gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc               >  1:109686/1‑250 (MQ=255)                                                                                                                                                                                                     gaccacgaccaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGAt                                                                                        <  1:673172/165‑1 (MQ=255)                                                                                                                                                                                                      accacgaccaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATc         <  1:499629/244‑1 (MQ=255)                                                                                                                                                                                                            accaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAg                                                                                                                               >  1:204601/1‑126 (MQ=255)                                                                                                                                                                                                            accaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAaatcga  >  1:103846/1‑251 (MQ=255)                                                                                                                                                                                                                 tCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTatgacgc                                                              <  1:526238/186‑7 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                CGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGATGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGACGCAACCGCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGA  >  1/729174‑729627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑