| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 84,041 | T→C | intergenic (+79434/+46464) | unknown → / ← unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 84,041 | 0 | T | C | 89.7% | 173.4 / ‑2.7 | 80 | intergenic (+79434/+46464) | unknown/unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base C (20/50); total (30/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACCACCGGCGCACCAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTTGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCAAAAAATCTTGGAAGA > 1/83797‑84283 | ccaccaCCGGCGCACCAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGGAATTCGTA‑ggg > 1:814777/1‑250 (MQ=255) cgcACCAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGt < 1:855509/250‑1 (MQ=255) cACCAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAg < 1:653075/247‑1 (MQ=255) ccAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGGGACAGCGGAAATCGGA‑GGGGCAACGAGGTGCAt > 1:755276/1‑251 (MQ=255) aaaaGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGGTT‑GGTAAAGCGAAAGCGCAAATTGGA‑GGGGGAACGAggtggattcctcaaaaaaaaaaat > 1:35496/1‑228 (MQ=255) ttcttcAAACACACCGTGGAAGATTACTTGTCACAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCGCGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTAcatcat < 1:748596/251‑1 (MQ=255) tcttcAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACatcatc < 1:682459/251‑1 (MQ=255) cttcAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATTGGTTAAGG‑GACAGGGCAATTAGTA‑AGGGCAACGAGGTGGCTCCCACCAGaaaaaaaaaattaaactatta > 1:94152/1‑237 (MQ=255) cttcAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GGTAAGGCGACAGCCGAAATAGTA‑GGGGCAAAGAGGTGCATCCTACaaaaaaaaaaaaaataaaacaaca > 1:857899/1‑237 (MQ=255) aaCACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑c < 1:819805/251‑1 (MQ=255) cacCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGaa < 1:385795/223‑1 (MQ=255) cacCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTg < 1:598512/251‑1 (MQ=255) gAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:815061/250‑1 (MQ=255) gAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:257533/250‑1 (MQ=255) gAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:541503/250‑1 (MQ=255) aaGATTGCTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAccc < 1:477979/250‑1 (MQ=255) aaGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAccc < 1:184281/250‑1 (MQ=255) gATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGGGATT‑GATAAGGCGGCAGCGGAATTCGTA‑GGGGCCACGAGGTGCATCCTCCAAAAAAAAAAAAAATAAATCATCAACAA‑CACTGT‑aaaaacctt > 1:629114/1‑245 (MQ=255) aCTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGCCAACGGAATTTGTA‑GGGGCAACGAGGTGCATCCTACAAGAAAAAAACAAGTACATCATCAACAA‑CCCTGT‑AAAaccccccctt > 1:37694/1‑245 (MQ=255) aTTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑TATAAAGCGACAGCGTAAATCGTA‑GGTGCAACGAGGTGCATCCTACAAGAAAAAAAAAAGTACATCATCAACAA‑CACTGT‑AAAACCCTCCCTTTCttaaaaaa > 1:647428/1‑244 (MQ=255) aTTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTAca < 1:272177/212‑1 (MQ=255) tttGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑c < 1:145172/222‑1 (MQ=255) aCCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAAc < 1:860778/189‑1 (MQ=255) aCCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CAc < 1:582538/209‑1 (MQ=255) ccTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAt < 1:861034/205‑1 (MQ=255) ccTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:36478/216‑1 (MQ=255) ccTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGCGACAGCGGAATTCGTA‑GGTGGAACGAGGTGCATCCTACAAAAAAAAAAAAAGTACATCATCAACAT‑CACTGT‑AAAACCCCCCCCTTCTGAAGAAAAAAAAAAAAAGTTAttt > 1:187803/1‑250 (MQ=255) ccTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGAGATT‑GATAAAGGGACAGCGGAAATCGTA‑GGGGCAACGAGGTGCATCCTACAAAAAAAAAAAAAGTACAACAACAAAAT‑CACTGT‑AAAAACCCCCGCTTCTGAaaaaaaaaaaaaaaaggtattt > 1:249707/1‑241 (MQ=255) cTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGGTT‑GATAAAGGGACAGCGGAAATTGTA‑GGGGCGACGAGGTGGATCCTACAAAAAAAAAAAAAGTAAATCATCAACAA‑CACTg > 1:708154/1‑209 (MQ=255) cTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTcc < 1:414133/251‑1 (MQ=255) ttATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTccc < 1:181703/251‑1 (MQ=255) tgCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATc < 1:438081/250‑1 (MQ=255) tgCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATc < 1:347272/250‑1 (MQ=255) cTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑TATAAGGCGACAGCGTAATTCGTA‑GGGGCAACGAGGTGCATCCTACAAGAAAAAAAAAAGTAAATCATCAACAC‑CACTGT‑AAACACCCCCGCTTCTGAAGAAAAAAAAAAAGAGATATTTCCAAACCAtgttggat > 1:554287/1‑246 (MQ=255) cTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAAt < 1:363634/251‑1 (MQ=255) cTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAAt < 1:825728/251‑1 (MQ=255) aTCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATc < 1:776209/239‑1 (MQ=255) aTCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGGGACAGCGTAATTCGTA‑GGGGCAAAGAGGTGCATCCTACAAAAAAAAAAAAAATACATCATCAAAAT‑CACTGT‑AAAcc > 1:568724/1‑198 (MQ=255) aTCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGCGACAGCGTAAATTGTA‑GGGGCAACGAGGTGCATCCCACAAAAAAAAAAAAAGTACATCATCAACAA‑CACTGT‑AAACCCCCCCCCTTCTTAAGaaaaaaaaaaaa > 1:207259/1‑223 (MQ=255) aTCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGCGACAGCGGAAATCGTAGGGGGCAAC‑AGTTGGCTCCTACAAGAAAAAAACAAGTACATCATCAACAT‑CCCCGTAAAACCCCCCCCTTTTTG‑AGAAAAAAAAAAACAGGTTTTTCCAATCCATGTGGGATgg > 1:460791/1‑251 (MQ=255) aTCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGGGAAT‑GATAAAGAGACAGCGGAAATTGTA‑AGGGGAAAGAATTGCCTCCTACAAGAAAAAAAAAAGTACATCATCAAAATCCAC‑GT‑AAAAACCTCCCCTTCTGTAAAAAAAAAAAAAAAAATATTTCCAATCCATGTGGaaagg > 1:60541/1‑248 (MQ=255) gcgcTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTAca < 1:224002/172‑1 (MQ=255) cTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑aaaaa < 1:328414/251‑1 (MQ=255) tGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGc < 1:541462/251‑1 (MQ=255) tGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGc < 1:162677/251‑1 (MQ=255) gcggcgTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGGGACAGCGGAAATCGTA‑GGGGCCACAAGATGCATCCCACAAAAAAAAAAAAAGTACAACATCAACAA‑CACTGT‑AAAACCCCCCCCTTCTTAAGAAAAAAAAAAAAAGATATTTCCAATCCCTGTGGAAAGGGTC‑aaaaaaaaaatccaaaca > 1:250264/1‑239 (MQ=255) gcgTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGGTT‑GATAAAGGGAAAGCGGAAATTGTA‑GGGGCAAAGAGGTGCATCCTACAAAAAAAAAAAAAGTAAATCATCAAAAA‑CACTGTAAAACCCCCCCGTTT‑TTAAGAAAAAAAAAAAAAGGTATTTCCAATACATGTGGAAAGGGTT‑aaaaaaaaattt > 1:190162/1‑236 (MQ=255) gcgTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTgg < 1:155872/222‑1 (MQ=255) gcgTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAAGCGACAGCGGAAATTGTA‑GGGGGAAAGAGGTGCATCCTACAAAAAAAAAAAAAGTACAACATCAACAT‑CACTGT‑AAAACCCCCCCCTTCTGAAGAAAAAAAAAAAGAGATATTCCCAAACCAtgtg > 1:790004/1‑221 (MQ=255) aaGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGaa < 1:331087/136‑1 (MQ=255) aTGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑TATAAAGCGACAGCGTAATTCGTA‑GGGGCAACGAGGTTCATCCTACAAGAAAAAAAAAAGTACATCATCAACAT‑CACTGT‑AAAACCCCCCCTTTCTTAAGAAAAAAAAAAAGAGGTATTCCCAATCCCTGTGGAATGGGTC‑AAAAAAAAATTCCAAACAAGCAAAattttt > 1:86096/1‑250 (MQ=255) ggACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTgg < 1:609586/208‑1 (MQ=255) aaGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTAc < 1:796605/251‑1 (MQ=255) cGAAATTCGCCGCACAAAAAGCCATGGCACCAGATCTCGCGGCACGTTTAGTGGCGATGATT‑GATAAGGCGTCAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCAGTCCtttca < 1:320381/249‑4 (MQ=255) cGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACa < 1:26867/249‑1 (MQ=255) cGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACAt < 1:697442/250‑1 (MQ=255) tCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGacac < 1:129615/250‑1 (MQ=255) gcACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:770235/136‑1 (MQ=255) caAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑TATAAAGCGACAGCGGAAATTGTA‑GGTGCAACGAGGTGCCTTCTACAAGAAAAAAACAATTACATCATCAACAT‑CACCGT‑AAAACCCCCCCCTTCTTAAAAAAAAAAAAAAAAACTATTTCCCATCCATTTTGGATGGGTCAAAAAAAAATTTCCAA‑CAGGCAAAATTTTTATTCTCGGTCCCTTCATCCACACCGATGccc > 1:334503/1‑250 (MQ=255) cATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCg < 1:176776/251‑1 (MQ=255) aGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACa < 1:614696/218‑1 (MQ=255) gTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAAcc < 1:258370/102‑1 (MQ=255) gTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAaaaacaaaac < 1:856230/128‑1 (MQ=255) gTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACa < 1:535616/204‑1 (MQ=255) gTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGacac < 1:271965/211‑1 (MQ=255) tttAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGc < 1:58494/250‑1 (MQ=255) cGGTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGc < 1:264839/250‑1 (MQ=255) gTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑aaaa < 1:533704/151‑1 (MQ=255) gTGATT‑GATAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTaa > 1:852992/1‑178 (MQ=255) tt‑gatAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCaa > 1:481861/1‑251 (MQ=255) gatAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGc > 1:468137/1‑200 (MQ=255) tAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGacac > 1:417950/1‑192 (MQ=255) tAAGGCGACAGCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCaaaaaa > 1:308584/1‑251 (MQ=255) gCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑aaaa < 1:849288/133‑1 (MQ=255) gCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGacac < 1:600468/182‑1 (MQ=255) gCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCAt > 1:609327/1‑232 (MQ=255) gCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCAAAAAATCTTGgaaga > 1:632550/1‑251 (MQ=255) gCGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCAAAAAATCTTGgaaga > 1:24704/1‑251 (MQ=255) cGTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCAAAAAATCTTGgaa < 1:735473/248‑1 (MQ=255) gTAATTCGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGa > 1:216355/1‑225 (MQ=255) | CCACCACCGGCGCACCAAATGAAGTGTATCAAAAAGACGGTTTCTTCAAACACACCGTGGAAGATTACTTGTCGCAATTTGAAACCTTCGCCACCTTATGTGCTTTGGACTATCAAGCGCTGGTCATCACCTGCGGCGTAAGCTATGCGGGACGAGACGAAGCGAAAGTCGCCGCACAAAAAGCCATGGCACCAGATCACGCGGCACGTTTAGTGGCGGTGATT‑GATAAGGCGACAGCGTAATTTGTA‑GGTGCAACGAGTTGCATCCTACAAGAAAAAAACATGTACATCATCAACAT‑CACTGT‑AAACCCCTCCGTTTCTGAAGAAAAACAAAACGAGTTATTCCCAATCCATGTGGAATGGTTC‑AAAAAATACTTCCAAGCAGGCAAATTTTTAATGCTCGGTCCTTACATCGACACCGATGCCCATTCAGGCGTCATTTTCGCGAAAACCGAAAGCCGTGAAGCATTCCAAAAAATCTTGGAAGA > 1/83797‑84283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |