Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	729,250	C→T	intergenic (‑6536/‑57941)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	729,250	0	C	T	100.0%	81.8 / NA	29	intergenic (‑6536/‑57941)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

CACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGAC  >  1/729011‑729493                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    catcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTgg                                                                                                                                                                                                                                          >  1:536541/4‑251 (MQ=255)           ccTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATccg                                                                                                                                                                                                                                >  1:450363/1‑250 (MQ=255)                 aaCAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCaa                                                                                                                                                                                                                          <  1:14588/251‑1 (MQ=255)                       cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                                                                    >  1:897422/1‑251 (MQ=255)                       cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                                                                    >  1:272634/1‑251 (MQ=255)                                     cAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTAtt                                                                                                                                                                                                      <  1:834540/251‑1 (MQ=255)                                          ggcgacatccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTgg                                                                                                                                                                                                 >  1:68449/17‑251 (MQ=255)                                                 tccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgc                                                                                                                                                                                          <  1:278547/242‑1 (MQ=255)                                                  ccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgcg                                                                                                                                                                                         >  1:443875/9‑251 (MQ=255)                                                          gagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCgatgat                                                                                                                                                                                 >  1:694667/1‑251 (MQ=255)                                                                                                                                                                    tgAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTc                                                                       <  1:234747/250‑1 (MQ=255)                                                                                                                                                                     gAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCa                                                                      >  1:856567/1‑251 (MQ=255)                                                                                                                                                                                cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                                            <  1:348820/250‑10 (MQ=255)                                                                                                                                                                                cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                                            <  1:803534/250‑10 (MQ=255)                                                                                                                                                                                  gTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccga                                                         >  1:265432/1‑251 (MQ=255)                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGTTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                        >  1:872472/5‑250 (MQ=255)                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                        >  1:408353/5‑250 (MQ=255)                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                        >  1:196735/5‑250 (MQ=255)                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                                                        >  1:777083/5‑250 (MQ=255)                                                                                                                                                                                      ataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGGTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGCTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgaccg                                                      >  1:879924/3‑250 (MQ=255)                                                                                                                                                                                                              cggAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACttttgttcagcgccgtcaatag                             <  1:401924/249‑20 (MQ=255)                                                                                                                                                                                                                       cgtttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGccc                        <  1:192737/236‑1 (MQ=255)                                                                                                                                                                                                                         tttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACg                  <  1:552992/242‑1 (MQ=255)                                                                                                                                                                                                                         tttatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACg                  <  1:513996/242‑1 (MQ=255)                                                                                                                                                                                                                           tatatcgccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTACACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGtt                >  1:580939/8‑251 (MQ=255)                                                                                                                                                                                                                                  ccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAAt                                  <  1:440071/226‑1 (MQ=255)                                                                                                                                                                                                                                  ccTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCa            <  1:260459/248‑1 (MQ=255)                                                                                                                                                                                                                                         aaCTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGAc  <  1:210795/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAAt                                  <  1:267978/218‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGAC  >  1/729011‑729493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑