Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	729,157	C→T	intergenic (‑6443/‑58034)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	729,157	0	C	T	100.0%	121.6 / NA	43	intergenic (‑6443/‑58034)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

CTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGAT  >  1/728911‑729318                                                                                                                                                                                                                                                       |                                                                                                                                                                  cTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTc                                                                                                                                                               <  1:204357/251‑1 (MQ=255) cTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTc                                                                                                                                                               <  1:515335/251‑1 (MQ=255)      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          <  1:578806/251‑1 (MQ=255)      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          <  1:65902/251‑1 (MQ=255)      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          <  1:396196/251‑1 (MQ=255)      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          <  1:471226/251‑1 (MQ=255)      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          <  1:660618/251‑1 (MQ=255)       gCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGa                                                                                                                                                         >  1:324372/1‑251 (MQ=255)                                   cccATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          >  1:782676/1‑222 (MQ=255)                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTAt                                                                                                                                                            <  1:89370/217‑1 (MQ=255)                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATAtttt                                                                                                                          <  1:128232/251‑1 (MQ=255)                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATAtttt                                                                                                                          <  1:246325/251‑1 (MQ=255)                                       tttATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTc                                                                                                                         <  1:116385/251‑1 (MQ=255)                                        ttATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCa                                                                                                                        >  1:795066/1‑251 (MQ=255)                                          aTCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACgg                                                                                                                                                      >  1:629920/1‑219 (MQ=255)                                                 tctgccgcccgcgcTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAAtttg                                                                                                               >  1:791685/11‑250 (MQ=255)                                                           gcgcTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCgctgcgga                                                                                                     >  1:853880/1‑244 (MQ=255)                                                                 gtcttCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                                                <  1:641658/247‑1 (MQ=255)                                                                     tCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGttt                                                                                           <  1:891812/251‑1 (MQ=255)                                                                      cccTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          >  1:471769/1‑187 (MQ=255)                                                                           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGAc                                                                                                                                                        <  1:613195/183‑1 (MQ=255)                                                                           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                                                <  1:837591/239‑1 (MQ=255)                                                                                     gTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGaa                                                                            >  1:77978/1‑250 (MQ=255)                                                                                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                           <  1:860768/250‑1 (MQ=255)                                                                                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                           <  1:177452/250‑1 (MQ=255)                                                                                         cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcg                                                                       >  1:579153/1‑251 (MQ=255)                                                                                         cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGACAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          >  1:71132/1‑168 (MQ=255)                                                                                           ttCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgt                                                                      >  1:832531/1‑249 (MQ=255)                                                                                                aactacatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaa                                                                 <  1:536029/242‑7 (MQ=255)                                                                                                    acatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaagattg                                                            >  1:529655/5‑240 (MQ=255)                                                                                                     catcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTgg                                                           >  1:536541/4‑251 (MQ=255)                                                                                                              aCCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                                          >  1:645381/1‑147 (MQ=255)                                                                                                               ccTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATccg                                                 >  1:450363/1‑250 (MQ=255)                                                                                                                    gAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTAtat                                                                                       >  1:418508/1‑206 (MQ=255)                                                                                                                     aaCAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCaa                                           <  1:14588/251‑1 (MQ=255)                                                                                                                         aacccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAg                                                                                                                                         <  1:724398/151‑1 (MQ=255)                                                                                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                     >  1:897422/1‑251 (MQ=255)                                                                                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                     >  1:272634/1‑251 (MQ=255)                                                                                                                                         cAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTAtt                       <  1:834540/251‑1 (MQ=255)                                                                                                                                              ggcgacatccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTgg                  >  1:68449/17‑251 (MQ=255)                                                                                                                                                     tccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgc           <  1:278547/242‑1 (MQ=255)                                                                                                                                                      ccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgcg          >  1:443875/9‑251 (MQ=255)                                                                                                                                                              gagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCgatgat  >  1:694667/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                  CTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGAT  >  1/728911‑729318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑