Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	728,647	T→C	intergenic (‑5933/‑58544)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	728,647	0	T	C	100.0%	159.4 / NA	56	intergenic (‑5933/‑58544)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/17);  total (39/17)

ACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTGCCGCCGGTGCGTTGTCGGAAGGCGGCACATTGTTGCTGTTATTCAATGAGTGGCAGGATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGAT  >  1/728405‑728889                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aCGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                                                                                                                                                                             >  1:889965/1‑247 (MQ=255)                gcatttggTTTTCA‑ACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCAc                                                                                                                                                                                                                                        <  1:123204/232‑1 (MQ=255)                   cGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCagcc                                                                                                                                                                                                                          <  1:367699/251‑4 (MQ=255)                                  cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                                                                                                                                                                             <  1:5892/217‑4 (MQ=255)                                  cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAg                                                                                                                                                                                                           >  1:572754/1‑251 (MQ=255)                                    aCGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCa                                                                                                                                                                                                         >  1:198051/1‑251 (MQ=255)                                               ttGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAAt                                                                                                                                                                                              <  1:108532/251‑1 (MQ=255)                                                                               ctctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCTCGCGCTTATTTa                                                                                                                                                              >  1:467338/1‑251 (MQ=255)                                                                                 ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCttatttat                                                                                                                                                             >  1:154465/1‑250 (MQ=255)                                                                                 ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                                                                                                                                            >  1:11930/1‑251 (MQ=255)                                                                                 ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                                                                                                                                            >  1:590757/1‑251 (MQ=255)                                                                                 ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                                                                                                                                            >  1:823761/1‑251 (MQ=255)                                                                                            aTAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGgcgtcag                                                                                                                                                 >  1:160657/1‑247 (MQ=255)                                                                                                   tattaaTGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTccc                                                                                                                                                                          >  1:577465/5‑219 (MQ=255)                                                                                                       aaTGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGgcgtcag                                                                                                                                                 >  1:558484/1‑236 (MQ=255)                                                                                                                 ggacggatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGctc                                                                                                                              >  1:28446/5‑247 (MQ=255)                                                                                                                      gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatga                                                                                                                                                           <  1:373470/215‑1 (MQ=255)                                                                                                                      gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATg                                                                                                                                 >  1:301001/1‑241 (MQ=255)                                                                                                                      gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGccat                                                                                                                       >  1:238826/1‑248 (MQ=255)                                                                                                                        tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAGGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatga                                                                                                                                                           >  1:437524/1‑213 (MQ=255)                                                                                                                        tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTc                                                                                                                                                                            >  1:647836/1‑196 (MQ=255)                                                                                                                        tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTg                                                                                                                     >  1:179552/1‑251 (MQ=255)                                                                                                                                            aaactaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGAt                                                                                                                                  >  1:492585/6‑218 (MQ=255)                                                                                                                                            aaactaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGaa                                                                                                 <  1:248402/246‑1 (MQ=255)                                                                                                                                              actaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGcc                                                                                                                         >  1:592869/4‑224 (MQ=255)                                                                                                                                               ctaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCAGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAgg                                                                                               >  1:212219/3‑250 (MQ=255)                                                                                                                                                          cagttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                    <  1:193059/234‑1 (MQ=255)                                                                                                                                                          cagttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                    <  1:300262/234‑1 (MQ=255)                                                                                                                                                           agttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                  >  1:870287/16‑251 (MQ=255)                                                                                                                                                           agttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                  >  1:470535/16‑251 (MQ=255)                                                                                                                                                            gttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                    <  1:271834/234‑1 (MQ=255)                                                                                                                                                             ttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                  <  1:56150/236‑1 (MQ=255)                                                                                                                                                                  tcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTAt                                                                           >  1:726572/9‑251 (MQ=255)                                                                                                                                                                    ttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTc                                                                         <  1:104009/245‑1 (MQ=255)                                                                                                                                                                        cattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatga                                                                                                                                                           >  1:23565/3‑165 (MQ=255)                                                                                                                                                                         attgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGa                                                                    <  1:867890/250‑1 (MQ=255)                                                                                                                                                                          ttgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGat                                                                   >  1:307769/1‑250 (MQ=255)                                                                                                                                                                          ttgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGa                                                                    <  1:14211/250‑1 (MQ=255)                                                                                                                                                                            ggtgacgaTGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                    >  1:411686/4‑232 (MQ=255)                                                                                                                                                                                        aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggc                                                     <  1:29227/251‑1 (MQ=255)                                                                                                                                                                                        aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTgg                                                      >  1:59991/1‑250 (MQ=255)                                                                                                                                                                                        aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTgg                                                      >  1:630816/1‑250 (MQ=255)                                                                                                                                                                                                   ccAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaac                                             >  1:2743/1‑242 (MQ=255)                                                                                                                                                                                                   ccAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaac                                             >  1:756796/1‑242 (MQ=255)                                                                                                                                                                                                       tGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatgg                                      <  1:63206/251‑14 (MQ=255)                                                                                                                                                                                                        gggaacattttCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatggg                                     >  1:253168/9‑237 (MQ=255)                                                                                                                                                                                                            acattttCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatggtgaca                                 >  1:309866/5‑233 (MQ=255)                                                                                                                                                                                                                      gCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGAt                                                                                                                                  >  1:27031/1‑144 (MQ=255)                                                                                                                                                                                                                       cccTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGt                                                                                                      >  1:71496/1‑171 (MQ=255)                                                                                                                                                                                                                                gTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGa                                                                                                                                                                                >  1:575030/1‑88 (MQ=255)                                                                                                                                                                                                                                gTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTGTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacc                                            <  1:893088/220‑8 (MQ=255)                                                                                                                                                                                                                                     gtatgGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAaca        >  1:235089/4‑251 (MQ=255)                                                                                                                                                                                                                                       atgGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGa                                                                                                  >  1:287215/2‑159 (MQ=255)                                                                                                                                                                                                                                         ggATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCg    >  1:212676/1‑251 (MQ=255)                                                                                                                                                                                                                                          gATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGa   >  1:210987/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGAt  <  1:642971/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTGCCGCCGGTGCGTTGTCGGAAGGCGGCACATTGTTGCTGTTATTCAATGAGTGGCAGGATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGAT  >  1/728405‑728889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑