Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	728,506	C→T	intergenic (‑5792/‑58685)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	728,506	0	C	T	100.0%	149.0 / NA	52	intergenic (‑5792/‑58685)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

CAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCGCGATCGTCTCAACGAACGCAAACGCCGGCAAGGCTTGGCGGGCGACAGTCGTTCTGTTGAAAACCAAGTGCAAAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAA  >  1/728260‑728746                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATg                                                                                                                                                                                                                                              <  1:716841/251‑1 (MQ=255) cAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGAGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATg                                                                                                                                                                                                                                              >  1:549811/1‑251 (MQ=255)   aTCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATg                                                                                                                                                                                                                                              <  1:392045/249‑1 (MQ=255)       cGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGatt                                                                                                                                                                                                                                        <  1:278285/251‑3 (MQ=255)         gtgtGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGt                                                                                                                                                                                                                                             <  1:714529/244‑1 (MQ=255)         gtgtGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtgg                                                                                                                                                                                                                                      >  1:472438/1‑251 (MQ=255)         gtgtGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCCCTATAAAAGCAATAAAAATATTAATGTAGatt                                                                                                                                                                                                                                        >  1:474416/1‑247 (MQ=255)           gtgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCGCTATAAAAGCAATAAAAATATTAATGTAGATtgga                                                                                                                                                                                                                                     >  1:158483/1‑250 (MQ=255)           gcgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtgga                                                                                                                                                                                                                                     >  1:321731/3‑250 (MQ=255)            tgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtggac                                                                                                                                                                                                                                    <  1:122577/250‑2 (MQ=255)            tgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtggac                                                                                                                                                                                                                                    <  1:126249/250‑2 (MQ=255)            tgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtggac                                                                                                                                                                                                                                    <  1:541652/250‑2 (MQ=255)            tgAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGAGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATtggac                                                                                                                                                                                                                                    <  1:645200/250‑2 (MQ=255)                tAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATc                                                                                                                                                                                                                               <  1:863042/251‑1 (MQ=255)                         tGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTcac                                                                                                                                                                                                                      <  1:508193/251‑3 (MQ=255)                           aaTTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTcacg                                                                                                                                                                                                                     <  1:106947/250‑4 (MQ=255)                                  aTGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGt                                                                                                                                                                                                             <  1:898787/251‑1 (MQ=255)                                      cgcgATTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTaaac                                                                                                                                                                                                         <  1:795635/251‑2 (MQ=255)                                          aTTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTaaactatg                                                                                                                                                                                                     >  1:783882/1‑246 (MQ=255)                                          aTTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGCAGATTGGACGGATCCGTATTc                                                                                                                                                                                                                        >  1:894766/1‑232 (MQ=255)                                          aTTTGAAACGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGGGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGGTGGTaaactatg                                                                                                                                                                                                     >  1:61574/1‑246 (MQ=255)                                                  cGGCATTTTCGCGATCGCCTCAACGAACGTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTaaactat                                                                                                                                                                                                      <  1:285007/242‑5 (MQ=255)                                                                       aacgaacgTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttctcattggtga                                                                                                                                                                        <  1:585950/251‑13 (MQ=255)                                                                           aacgTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGAt                                                                                                                                                                    >  1:259635/1‑251 (MQ=255)                                                                             cgtaaaCGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTaaacta                                                                                                                                                                                                       <  1:42119/211‑4 (MQ=255)                                                                                   cGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGtt                                                                                                                                                            <  1:42654/251‑1 (MQ=255)                                                                                     ccgtcAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGt                                                                                                                                                                                                                                             <  1:515434/164‑1 (MQ=255)                                                                                       gtcAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAgcg                                                                                                                                                        >  1:709852/3‑251 (MQ=255)                                                                                                     ggtgACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttc                                                                                                                                                                                  <  1:88790/208‑3 (MQ=255)                                                                                                             tCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGaacatttt                                                                                                                                      <  1:444568/247‑7 (MQ=255)                                                                                                               gTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGc                                                                                                                                 >  1:486308/1‑250 (MQ=255)                                                                                                                    gTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGt                                                                                                                                                                                                                                             <  1:893543/137‑1 (MQ=255)                                                                                                                          aaCCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTg                                                                                                                      >  1:246518/1‑250 (MQ=255)                                                                                                                           aCCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCg                                                                                                                    >  1:573032/1‑251 (MQ=255)                                                                                                                               aatGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGgtat                                                                                                                <  1:53669/249‑3 (MQ=255)                                                                                                                                      aaGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGAt                                                                                                            <  1:210647/248‑1 (MQ=255)                                                                                                                                         cAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATTCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCgg                                                                                                      <  1:214952/251‑1 (MQ=255)                                                                                                                                                  aCGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATc                                                                                                                                                                                                                               >  1:342901/1‑121 (MQ=255)                                                                                                                                                  aCGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                              >  1:889965/1‑247 (MQ=255)                                                                                                                                                                 gcatttggTTTTCA‑ACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCAc                                                                                         <  1:123204/232‑1 (MQ=255)                                                                                                                                                                    cGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCagcc                                                                           <  1:367699/251‑4 (MQ=255)                                                                                                                                                                                   cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                              <  1:5892/217‑4 (MQ=255)                                                                                                                                                                                   cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAg                                                            >  1:572754/1‑251 (MQ=255)                                                                                                                                                                                     aCGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCa                                                          >  1:198051/1‑251 (MQ=255)                                                                                                                                                                                          aaTCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttctcattggtga                                                                                                                                                                        >  1:164543/1‑124 (MQ=255)                                                                                                                                                                                                ttGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAAt                                               <  1:108532/251‑1 (MQ=255)                                                                                                                                                                                                                                ctctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCTCGCGCTTATTTa               >  1:467338/1‑251 (MQ=255)                                                                                                                                                                                                                                  ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCttatttat              >  1:154465/1‑250 (MQ=255)                                                                                                                                                                                                                                  ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg             >  1:590757/1‑251 (MQ=255)                                                                                                                                                                                                                                  ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg             >  1:823761/1‑251 (MQ=255)                                                                                                                                                                                                                                  ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg             >  1:11930/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGgcgtcag  >  1:160657/1‑247 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCGCGATCGTCTCAACGAACGCAAACGCCGGCAAGGCTTGGCGGGCGACAGTCGTTCTGTTGAAAACCAAGTGCAAAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAA  >  1/728260‑728746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑