Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	728,092	A→G	intergenic (‑5378/‑59099)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	728,092	0	A	G	100.0%	207.8 / NA	70	intergenic (‑5378/‑59099)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/26);  total (44/26)

CTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCGCGATCGTCTCAACGAA  >  1/727858‑728335                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    cttacttaTTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaag                                                                                                                                                                                                                                      >  1:586646/1‑250 (MQ=255)   tacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagcaa                                                                                                                                                                                                                                   >  1:664378/1‑251 (MQ=255)        attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                                              >  1:126569/2‑251 (MQ=255)        attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                                              >  1:267454/2‑251 (MQ=255)                    tttttttaaaCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAGCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                                  <  1:783462/243‑1 (MQ=255)                     cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagca                                                                                                                                                                                                                                    >  1:162855/1‑232 (MQ=255)                     cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                                         >  1:157371/1‑227 (MQ=255)                     cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTAAGCAAAAAAAAACTTGGAATTTTGTTGGTTGCGGGAATTGGGAAAGAAATGGGACGAACGTGTTatt                                                                                                                                                                                                                 >  1:157874/1‑249 (MQ=255)                       aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                                >  1:421049/1‑248 (MQ=255)                       aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                                >  1:335878/1‑248 (MQ=255)                               cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGTTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                                <  1:804429/242‑3 (MQ=255)                               cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                                                                                                                                                                                                       >  1:107284/1‑251 (MQ=255)                                           ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTATTGCCCTTGAAAAACGCTTATTaa                                                                                                                                                                                           >  1:412543/1‑251 (MQ=255)                                           ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACgcttatta                                                                                                                                                                                            <  1:521803/250‑8 (MQ=255)                                            tattatCCTTATCCGCAATTTGACCCTGGTTACCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaa                                                                                                                                                                                          <  1:585978/251‑1 (MQ=255)                                            tattatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                                  <  1:248727/227‑1 (MQ=255)                                             attatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaaa                                                                                                                                                                                         >  1:136211/1‑251 (MQ=255)                                                atccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGat                                                                                                                                                                                      <  1:474733/251‑1 (MQ=255)                                                 tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                                         <  1:312469/199‑1 (MQ=255)                                                      atccGCAATTTGACCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                                <  1:802080/251‑1 (MQ=255)                                                             aTTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACg                                                                                                                                                                         >  1:895388/1‑251 (MQ=255)                                                                      ctggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                                <  1:100859/188‑3 (MQ=255)                                                                       tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGa                                                                                                                                                                                       >  1:841763/16‑228 (MQ=255)                                                                       tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGa                                                                                                                                                                >  1:810757/16‑251 (MQ=255)                                                                        ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                                >  1:155253/15‑234 (MQ=255)                                                                        ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGaa                                                                                                                                                               >  1:13011/15‑251 (MQ=255)                                                                             tccgcccccttCCGTGCAGCATAATAATGATGAAGAAAAGGCCGTTTTCAGAACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGt                                                                                                                                                                      <  1:677864/230‑1 (MQ=255)                                                                                ggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTgg                                                                                                                                                       >  1:735009/7‑251 (MQ=255)                                                                                   cccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCa                                                                                                                                                    >  1:803649/4‑251 (MQ=255)                                                                                     cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                                                                                                                                  >  1:585668/2‑251 (MQ=255)                                                                                     cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                                                                                                                                  >  1:591671/2‑251 (MQ=255)                                                                                      ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                                                                                                                                 <  1:99062/251‑2 (MQ=255)                                                                                      ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                                                                                                                                 <  1:689318/251‑2 (MQ=255)                                                                                        ccGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaacca                                                                                                                                               <  1:33720/251‑4 (MQ=255)                                                                                          gTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                                  >  1:778236/1‑182 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                                         >  1:409028/1‑154 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                                              >  1:417308/1‑165 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGAt                                                                                                                                                            <  1:760806/231‑1 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaaccatcgg                                                                                                                                           >  1:877428/1‑241 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:663833/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:311624/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:485292/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:841660/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:14263/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:590613/1‑250 (MQ=255)                                                                                               gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                                         >  1:359877/1‑250 (MQ=255)                                                                                                  tAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGcgcgca                                                                                                                                     >  1:344240/1‑249 (MQ=255)                                                                                                         gATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                                  >  1:585590/1‑167 (MQ=255)                                                                                                                aaaaTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACg                                                                                                                         <  1:408953/246‑1 (MQ=255)                                                                                                                   aTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAg                                                                                                                    <  1:219933/251‑1 (MQ=255)                                                                                                                       ctttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACAAGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtctt                                                                                                                <  1:186546/249‑1 (MQ=255)                                                                                                                         ttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtcttc                                                                                                               <  1:22906/250‑1 (MQ=255)                                                                                                                          tttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTcttctt                                                                                                             >  1:78169/1‑251 (MQ=255)                                                                                                                                cacccgccgCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                                            >  1:816875/4‑246 (MQ=255)                                                                                                                                           cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                                            >  1:536522/1‑235 (MQ=255)                                                                                                                                           cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAAttt                                                                                            >  1:869396/1‑251 (MQ=255)                                                                                                                                           cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAAttt                                                                                            >  1:195905/1‑251 (MQ=255)                                                                                                                                                        atatCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATcaacaa                                                                                >  1:610534/1‑250 (MQ=255)                                                                                                                                                        atatCAGGATCCGAGACACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                                                                                                                                                                                                       <  1:302209/131‑1 (MQ=255)                                                                                                                                                          atCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                                >  1:694235/1‑152 (MQ=255)                                                                                                                                                                aTCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCAc                                                                       <  1:676342/251‑1 (MQ=255)                                                                                                                                                                           aaaaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATaa                                                            <  1:823312/251‑1 (MQ=255)                                                                                                                                                                             aaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                                            <  1:623237/201‑1 (MQ=255)                                                                                                                                                                             aaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACa                                                          <  1:306521/251‑1 (MQ=255)                                                                                                                                                                                                            ttAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACg                           <  1:49425/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCAtttt                    >  1:156926/1‑250 (MQ=255)                                                                                                                                                                                                                     aaTAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTcg                  <  1:254921/251‑1 (MQ=255)                                                                                                                                                                                                                             ggAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTggc                                                                                                                                                                  >  1:816754/1‑97 (MQ=255)                                                                                                                                                                                                                             ggAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACg                                                                                                          <  1:597034/155‑1 (MQ=255)                                                                                                                                                                                                                                     gtttgttGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTCGCGATCGCCTCaacgaa  >  1:347133/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    CTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCGCGATCGTCTCAACGAA  >  1/727858‑728335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑