Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	728,075	C→T	intergenic (‑5361/‑59116)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	728,075	0	C	T	100.0%	204.9 / NA	71	intergenic (‑5361/‑59116)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/25);  total (46/25)

GGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCG  >  1/727836‑728319                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ggAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttgt                                                                                                                                                                                                                                           >  1:417865/1‑251 (MQ=255)                       cttacttaTTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaag                                                                                                                                                                                                                      >  1:586646/1‑250 (MQ=255)                         tacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagcaa                                                                                                                                                                                                                   >  1:664378/1‑251 (MQ=255)                           cttattttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttg                                                                                                                                                                                                                                            >  1:318695/5‑224 (MQ=255)                              attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                              >  1:126569/2‑251 (MQ=255)                              attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                              >  1:267454/2‑251 (MQ=255)                                          tttttttaaaCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAGCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                  <  1:783462/243‑1 (MQ=255)                                           cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAatat                                                                                                                                                                                                                                              >  1:161632/1‑206 (MQ=255)                                           cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagca                                                                                                                                                                                                                    >  1:162855/1‑232 (MQ=255)                                           cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                         >  1:157371/1‑227 (MQ=255)                                           cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTAAGCAAAAAAAAACTTGGAATTTTGTTGGTTGCGGGAATTGGGAAAGAAATGGGACGAACGTGTTatt                                                                                                                                                                                                 >  1:157874/1‑249 (MQ=255)                                             aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                >  1:421049/1‑248 (MQ=255)                                             aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                >  1:335878/1‑248 (MQ=255)                                                     cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGTTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                <  1:804429/242‑3 (MQ=255)                                                     cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                                                                                                                                                                                       >  1:107284/1‑251 (MQ=255)                                                                 ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTATTGCCCTTGAAAAACGCTTATTaa                                                                                                                                                                           >  1:412543/1‑251 (MQ=255)                                                                 ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACgcttatta                                                                                                                                                                            <  1:521803/250‑8 (MQ=255)                                                                  tattatCCTTATCCGCAATTTGACCCTGGTTACCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaa                                                                                                                                                                          <  1:585978/251‑1 (MQ=255)                                                                  tattatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                  <  1:248727/227‑1 (MQ=255)                                                                   attatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaaa                                                                                                                                                                         >  1:136211/1‑251 (MQ=255)                                                                      atccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGat                                                                                                                                                                      <  1:474733/251‑1 (MQ=255)                                                                       tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                         <  1:312469/199‑1 (MQ=255)                                                                            atccGCAATTTGACCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                <  1:802080/251‑1 (MQ=255)                                                                                   aTTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACg                                                                                                                                                         >  1:895388/1‑251 (MQ=255)                                                                                            ctggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                                                                                                                <  1:100859/188‑3 (MQ=255)                                                                                             tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGa                                                                                                                                                                       >  1:841763/16‑228 (MQ=255)                                                                                             tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGa                                                                                                                                                >  1:810757/16‑251 (MQ=255)                                                                                              ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                >  1:155253/15‑234 (MQ=255)                                                                                              ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGaa                                                                                                                                               >  1:13011/15‑251 (MQ=255)                                                                                                   tccgcccccttCCGTGCAGCATAATAATGATGAAGAAAAGGCCGTTTTCAGAACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGt                                                                                                                                                      <  1:677864/230‑1 (MQ=255)                                                                                                      ggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttg                                                                                                                                                                                                                                            >  1:524781/7‑150 (MQ=255)                                                                                                      ggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTgg                                                                                                                                       >  1:735009/7‑251 (MQ=255)                                                                                                         cccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCa                                                                                                                                    >  1:803649/4‑251 (MQ=255)                                                                                                           cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                                                                                                                  >  1:585668/2‑251 (MQ=255)                                                                                                           cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                                                                                                                  >  1:591671/2‑251 (MQ=255)                                                                                                            ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                                                                                                                 <  1:99062/251‑2 (MQ=255)                                                                                                            ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                                                                                                                 <  1:689318/251‑2 (MQ=255)                                                                                                              ccGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaacca                                                                                                                               <  1:33720/251‑4 (MQ=255)                                                                                                                gTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                  >  1:778236/1‑182 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                                                                                                                         >  1:409028/1‑154 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                                                                                                              >  1:417308/1‑165 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGAt                                                                                                                                            <  1:760806/231‑1 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaaccatcgg                                                                                                                           >  1:877428/1‑241 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:485292/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:14263/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:841660/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:311624/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:663833/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:590613/1‑250 (MQ=255)                                                                                                                     gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                                                                                                                         >  1:359877/1‑250 (MQ=255)                                                                                                                        tAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGcgcgca                                                                                                                     >  1:344240/1‑249 (MQ=255)                                                                                                                               gATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                                                                                                                  >  1:585590/1‑167 (MQ=255)                                                                                                                                      aaaaTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACg                                                                                                         <  1:408953/246‑1 (MQ=255)                                                                                                                                         aTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAg                                                                                                    <  1:219933/251‑1 (MQ=255)                                                                                                                                             ctttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACAAGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtctt                                                                                                <  1:186546/249‑1 (MQ=255)                                                                                                                                               ttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtcttc                                                                                               <  1:22906/250‑1 (MQ=255)                                                                                                                                                tttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTcttctt                                                                                             >  1:78169/1‑251 (MQ=255)                                                                                                                                                      cacccgccgCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                            >  1:816875/4‑246 (MQ=255)                                                                                                                                                                 cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                            >  1:536522/1‑235 (MQ=255)                                                                                                                                                                 cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAAttt                                                                            >  1:869396/1‑251 (MQ=255)                                                                                                                                                                 cgcaTGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAAttt                                                                            >  1:195905/1‑251 (MQ=255)                                                                                                                                                                              atatCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATcaacaa                                                                >  1:610534/1‑250 (MQ=255)                                                                                                                                                                              atatCAGGATCCGAGACACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                                                                                                                                                                                       <  1:302209/131‑1 (MQ=255)                                                                                                                                                                                atCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                                                                                                                >  1:694235/1‑152 (MQ=255)                                                                                                                                                                                      aTCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCAc                                                       <  1:676342/251‑1 (MQ=255)                                                                                                                                                                                                 aaaaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATaa                                            <  1:823312/251‑1 (MQ=255)                                                                                                                                                                                                   aaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTa                                                                                            <  1:623237/201‑1 (MQ=255)                                                                                                                                                                                                   aaTTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACa                                          <  1:306521/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACg           <  1:49425/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaaTAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCAtttt    >  1:156926/1‑250 (MQ=255)                                                                                                                                                                                                                                           aaTAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCATTTTcg  <  1:254921/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTTACGATTATTTTCAATTTAAGTATCAACAACTCAATCACGGTGTGAATAACATTCGTGAATTTGAATGCCGCGATTTGAAACGGCACTTTCG  >  1/727836‑728319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑