Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	727,986	T→C	intergenic (‑5272/‑59205)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	727,986	0	T	C	100.0%	186.8 / NA	65	intergenic (‑5272/‑59205)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/19);  total (46/19)

CATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTT  >  1/727763‑728228                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   tatatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCa                                                                                                                                                                                                                         >  1:135765/2‑251 (MQ=255)  atatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                                                                                                                                        >  1:693307/1‑251 (MQ=255)  atatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                                                                                                                                        >  1:231242/1‑251 (MQ=255)       gTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATc                                                                                                                                                                                                                    >  1:357354/1‑250 (MQ=255)            acGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGAt                                                                                                                                                                                                              >  1:215132/1‑251 (MQ=255)                                         gcgggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGaaaaa                                                                                                                                                                                 >  1:772262/7‑251 (MQ=255)                                            ggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAAtt                                                                                                                                                                               >  1:23965/4‑250 (MQ=255)                                                                          ggAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttgt                                                                                                                                                >  1:417865/1‑251 (MQ=255)                                                                                                cttacttaTTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaag                                                                                                                           >  1:586646/1‑250 (MQ=255)                                                                                                  tacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagcaa                                                                                                                        >  1:664378/1‑251 (MQ=255)                                                                                                    cttattttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttg                                                                                                                                                 >  1:318695/5‑224 (MQ=255)                                                                                                       attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                   >  1:126569/2‑251 (MQ=255)                                                                                                       attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                   >  1:267454/2‑251 (MQ=255)                                                                                                                   tttttttaaaCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAGCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                       <  1:783462/243‑1 (MQ=255)                                                                                                                    cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAatat                                                                                                                                                   >  1:161632/1‑206 (MQ=255)                                                                                                                    cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagca                                                                                                                         >  1:162855/1‑232 (MQ=255)                                                                                                                    cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                              >  1:157371/1‑227 (MQ=255)                                                                                                                    cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTAAGCAAAAAAAAACTTGGAATTTTGTTGGTTGCGGGAATTGGGAAAGAAATGGGACGAACGTGTTatt                                                                                                      >  1:157874/1‑249 (MQ=255)                                                                                                                      aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                     >  1:421049/1‑248 (MQ=255)                                                                                                                      aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                     >  1:335878/1‑248 (MQ=255)                                                                                                                              cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGTTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                     <  1:804429/242‑3 (MQ=255)                                                                                                                              cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                                                                                            >  1:107284/1‑251 (MQ=255)                                                                                                                                          ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTATTGCCCTTGAAAAACGCTTATTaa                                                                                >  1:412543/1‑251 (MQ=255)                                                                                                                                          ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACgcttatta                                                                                 <  1:521803/250‑8 (MQ=255)                                                                                                                                           tattatCCTTATCCGCAATTTGACCCTGGTTACCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaa                                                                               <  1:585978/251‑1 (MQ=255)                                                                                                                                           tattatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                       <  1:248727/227‑1 (MQ=255)                                                                                                                                            attatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaaa                                                                              >  1:136211/1‑251 (MQ=255)                                                                                                                                               atccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGat                                                                           <  1:474733/251‑1 (MQ=255)                                                                                                                                                tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                              <  1:312469/199‑1 (MQ=255)                                                                                                                                                tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGAATAATGATGATGAAGAAAATGCCGTTTTCAGCGCCCGCCGCACGCATGAACCGAAATATc                                                                                                                                                                                                                          <  1:185424/107‑1 (MQ=255)                                                                                                                                                     atccGCAATTTGACCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                     <  1:802080/251‑1 (MQ=255)                                                                                                                                                            aTTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACg                                                              >  1:895388/1‑251 (MQ=255)                                                                                                                                                                     ctggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                                                                                     <  1:100859/188‑3 (MQ=255)                                                                                                                                                                      tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGa                                                                            >  1:841763/16‑228 (MQ=255)                                                                                                                                                                      tggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGa                                                     >  1:810757/16‑251 (MQ=255)                                                                                                                                                                       ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc                                                                     >  1:155253/15‑234 (MQ=255)                                                                                                                                                                       ggttatccggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGaa                                                    >  1:13011/15‑251 (MQ=255)                                                                                                                                                                            tccgcccccttCCGTGCAGCATAATAATGATGAAGAAAAGGCCGTTTTCAGAACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGt                                                           <  1:677864/230‑1 (MQ=255)                                                                                                                                                                               ggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttg                                                                                                                                                 >  1:524781/7‑150 (MQ=255)                                                                                                                                                                               ggtcccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTgg                                            >  1:735009/7‑251 (MQ=255)                                                                                                                                                                                  cccttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCa                                         >  1:803649/4‑251 (MQ=255)                                                                                                                                                                                    cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                       >  1:585668/2‑251 (MQ=255)                                                                                                                                                                                    cttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaa                                       >  1:591671/2‑251 (MQ=255)                                                                                                                                                                                     ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                      <  1:99062/251‑2 (MQ=255)                                                                                                                                                                                     ttCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaac                                      <  1:689318/251‑2 (MQ=255)                                                                                                                                                                                       ccGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaacca                                    <  1:33720/251‑4 (MQ=255)                                                                                                                                                                                         gTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                       >  1:778236/1‑182 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                                                                                              >  1:409028/1‑154 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                                                                                   >  1:417308/1‑165 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGAt                                                 <  1:760806/231‑1 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCaaaccatcgg                                >  1:877428/1‑241 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:663833/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:14263/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:841660/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:311624/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:359877/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:485292/1‑250 (MQ=255)                                                                                                                                                                                              gcaTAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGgcg                              >  1:590613/1‑250 (MQ=255)                                                                                                                                                                                                 tAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGcgcgca                          >  1:344240/1‑249 (MQ=255)                                                                                                                                                                                                        gATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                                                                                       >  1:585590/1‑167 (MQ=255)                                                                                                                                                                                                               aaaaTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACg              <  1:408953/246‑1 (MQ=255)                                                                                                                                                                                                                  aTGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAg         <  1:219933/251‑1 (MQ=255)                                                                                                                                                                                                                      ctttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACAAGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtctt     <  1:186546/249‑1 (MQ=255)                                                                                                                                                                                                                        ttttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGtcttc    <  1:22906/250‑1 (MQ=255)                                                                                                                                                                                                                         tttCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATCAATGACGAGTTGGCGAAGATTGTGGGCAAACCATCGGCGCGCACGATTTTTGACGAGCAGTcttctt  >  1:78169/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   CATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAAACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATCAATGACGAGTTGTCGAAGATTGTGGGCAAAGCGTCGGCGCGTACGATTTTTGACGATCAGTCTTCTT  >  1/727763‑728228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑