Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	727,808	G→A	intergenic (‑5094/‑59383)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	727,808	0	G	A	100.0%	138.3 / NA	48	intergenic (‑5094/‑59383)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/28);  total (20/28)

CATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGAT  >  1/727564‑728024                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          ttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAtt                                                                                                                                                                                                                   <  1:845801‑M2/62‑1 (MQ=255)     atttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                                                               <  1:761325‑M2/66‑1 (MQ=255)         actcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTAtt                                                                                                                                                                                                           >  1:66273‑M2/182‑251 (MQ=255)             ggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCa                                                                                                                                                                                                       >  1:472576‑M2/178‑251 (MQ=255)              gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAt                                                                                                                                                                                                                    >  1:150714‑M2/177‑237 (MQ=255)              gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                                                               <  1:855640‑M2/66‑1 (MQ=255)                   gatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGa                                                                                                                                                                                                 >  1:471959‑M2/172‑251 (MQ=255)                    atcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGaa                                                                                                                                                                                                >  1:446602‑M2/171‑251 (MQ=255)                       atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                                                             >  1:690648‑M2/168‑251 (MQ=255)                       atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                                                             >  1:722156‑M2/168‑251 (MQ=255)                          ttgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAata                                                                                                                                                                                          <  1:426788‑M2/87‑1 (MQ=255)                                atgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGt                                                                                                                                                                                    <  1:842769‑M2/93‑1 (MQ=255)                                 tgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                                                                         >  1:204574‑M2/158‑245 (MQ=255)                                            aggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtac                                                                                                                                                                         <  1:702070‑M2/104‑1 (MQ=255)                                             ggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                                                                         <  1:333155‑M2/88‑1 (MQ=255)                                               aaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtacttac                                                                                                                                                                     <  1:187543‑M2/108‑1 (MQ=255)                                                                                    cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                              <  1:189979/250‑1 (MQ=255)                                                                                          cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:386685/251‑1 (MQ=255)                                                                                           gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:43973/250‑1 (MQ=255)                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCt                                                                                                                                                                                                              <  1:460939/162‑1 (MQ=255)                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAgg                                                                                                                                                                                              <  1:391927/178‑1 (MQ=255)                                                                                               atccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatc                                                                                                                        >  1:444596‑M2/96‑248 (MQ=255)                                                                                               atccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                              <  1:580545‑M2/147‑1 (MQ=255)                                                                                                gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                                                                                          >  1:42256/1‑210 (MQ=255)                                                                                                    tatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCg                                                                                                                >  1:711569‑M2/91‑251 (MQ=255)                                                                                                             ggagatgtgtataagaaaaattgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCA‑‑TA‑A‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATTTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAAcc                                                                                                     <  1:522820/229‑1 (MQ=255)                                                                                                                     aaaaGTCGGAAAGTGAAAAACTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                                                            <  1:223172/251‑1 (MQ=255)                                                                                                                             gaaagtgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAat                                                                                                                                                                                           <  1:424091/144‑1 (MQ=255)                                                                                                                                    tAAAACTATATCGAAAAAACAGGGCCAACGGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATCCAGGTTTTTAGGGTGGCGCCAGCGGAACAATGATTATTCTATTTTCACCCTAAAGGAATATCATTTTAAAAAAATACTTGTTTATTTTACCGTTTTGTATTTAAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcccttccgtgc                                                                             <  1:508630/250‑14 (MQ=255)                                                                                                                                    gtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAAc                                                                                                                                            <  1:771184‑M2/133‑1 (MQ=255)                                                                                                                                    gtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTatcc‑ggtcc                                                                                      <  1:396556‑M2/186‑5 (MQ=255)                                                                                                                                             ttCGGAAAAACAGGTTAAAACGGGAATTCTTGCTTCATTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTAAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:810714/251‑1 (MQ=255)                                                                                                                                             ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:710923/251‑1 (MQ=255)                                                                                                                                                 gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:143318/239‑1 (MQ=255)                                                                                                                                                         gggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:330856/188‑1 (MQ=255)                                                                                                                                                           gTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATg                                                     >  1:327642/1‑251 (MQ=255)                                                                                                                                                                  gggCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGtttt                                              >  1:153639/1‑251 (MQ=255)                                                                                                                                                                             tcttgcttcattttccattaaaaGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTGTTATTTTAGCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATaa                                                                      <  1:258853/201‑1 (MQ=255)                                                                                                                                                                                             attaaaaGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATc                                                                                                                                                                                        <  1:338663/88‑1 (MQ=255)                                                                                                                                                                                                    gCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   >  1:593899/1‑201 (MQ=255)                                                                                                                                                                                                       atatatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGatg                                                                 <  1:762781/198‑3 (MQ=255)                                                                                                                                                                                                        tatatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCa             >  1:135765/2‑251 (MQ=255)                                                                                                                                                                                                         atatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   >  1:819786/1‑196 (MQ=255)                                                                                                                                                                                                         atatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg            >  1:693307/1‑251 (MQ=255)                                                                                                                                                                                                         atatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg            >  1:231242/1‑251 (MQ=255)                                                                                                                                                                                                              gTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATc        >  1:357354/1‑250 (MQ=255)                                                                                                                                                                                                                   acttAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTAAAGAAATATCATGTTAAAAAAATACTTACTTATTTTACCGTTTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:840948/183‑1 (MQ=255)                                                                                                                                                                                                                   acGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGAt  >  1:215132/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          CATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGAT  >  1/727564‑728024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑