Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	67,593	A→C	intergenic (+62986/+62912)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	67,593	0	A	C	100.0%	106.8 / NA	39	intergenic (+62986/+62912)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/21);  total (18/21)

CGCATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCACTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCATCCCGGTCTCCATAATTA  >  1/67360‑67672                                                                                                                                                                                                                                          |                                                                                cgcATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAGTACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:354207/251‑1 (MQ=255)     tGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAg                                                            >  1:164299/1‑251 (MQ=255)      gTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATcc                                                                     <  1:573903/241‑1 (MQ=255)                cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:827849/251‑1 (MQ=255)                cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:499309/251‑1 (MQ=255)                cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:331235/251‑1 (MQ=255)                cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:360720/251‑1 (MQ=255)                    cGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCt                                             <  1:726901/251‑1 (MQ=255)                              cGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:252091/251‑1 (MQ=255)                              cGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:43794/251‑1 (MQ=255)                               gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:632890/215‑1 (MQ=255)                               gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAgg                                                           >  1:781365/1‑226 (MQ=255)                               gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATa                                    >  1:569986/1‑249 (MQ=255)                                   tGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTAtt                              <  1:86089/251‑1 (MQ=255)                                     aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:807898/209‑1 (MQ=255)                                     aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAg                                                         <  1:325952/222‑1 (MQ=255)                                     aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTg                                                    >  1:5689/1‑227 (MQ=255)                                                             aTGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:384494/185‑1 (MQ=255)                                                             aTGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             <  1:125963/226‑1 (MQ=255)                                                                aaTACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacgagcgttgcagcac  <  1:450944/250‑18 (MQ=255)                                                                               aaTTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:130264/1‑208 (MQ=255)                                                                                                         ttCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:133245/147‑1 (MQ=255)                                                                                                         ttCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACtt                                 >  1:401412/1‑178 (MQ=255)                                                                                                                       gTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacg               >  1:849206/1‑178 (MQ=255)                                                                                                                              ccACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacgagcgttgcagcac  >  1:719537/1‑171 (MQ=255)                                                                                                                                                    gTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:303084/104‑1 (MQ=255)                                                                                                                                                        aGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacg               <  1:404948/149‑5 (MQ=255)                                                                                                                                                             ggttTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTtat                                                                         >  1:464338/1‑86 (MQ=255)                                                                                                                                                             ggttTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTc                                              >  1:375841/1‑113 (MQ=255)                                                                                                                                                                  gAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      >  1:653142/1‑84 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAgg                                                           <  1:271094/90‑1 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTa                                                  >  1:533406/1‑99 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATg                                         >  1:226797/1‑108 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGaaa                                      >  1:789542/1‑111 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   >  1:827047/1‑114 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:597136/114‑1 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:190763/1‑120 (MQ=255)                                                                                                                                                                       gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:118930/1‑120 (MQ=255)                                                                                                                                                                                aGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAAt                         >  1:401954/1‑115 (MQ=255)                                                                                                                                                                                                                                          |                                                                                CGCATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCACTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCATCCCGGTCTCCATAATTA  >  1/67360‑67672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑