Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	727,763	C→T	intergenic (‑5049/‑59428)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	727,763	0	C	T	100.0%	146.1 / NA	54	intergenic (‑5049/‑59428)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

CCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACA‑CGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTT  >  1/727519‑727980                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                            aaaccgctcatggcgcattgggcacacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAgccatatatggt                                                                                                                                                                                                                      <  1:591276‑M2/16‑4 (MQ=255) aaaccgctcatggcgcattgggcacacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGccatatatgg                                                                                                                                                                                                                       <  1:411858‑M2/15‑3 (MQ=255)                    gggcacacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAgccatatatggt                                                                                                                                                                                                                      >  1:781562‑M2/216‑228 (MQ=255)                    gggcacacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCtttt                                                                                                                                                                                                 >  1:530022‑M2/216‑251 (MQ=255)                         cacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGt                                                                                                                                                                                            >  1:115923‑M2/211‑251 (MQ=255)                         cacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGt                                                                                                                                                                                            >  1:797600‑M2/211‑251 (MQ=255)                          acatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATtcaggct                                                                                                                                                                                                    <  1:246222‑M2/33‑4 (MQ=255)                           catggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAgccatatatggt                                                                                                                                                                                                                      >  1:637499‑M2/209‑221 (MQ=255)                               gtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCatatat                                                                                                                                                                                                                         >  1:348005‑M2/205‑217 (MQ=255)                               gtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAg                                                                                                                                                                                              >  1:897391‑M2/205‑243 (MQ=255)                                         gcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATACCGTAATTCAGGCTTTTAAGGTTGCTGcagcgggac                                                                                                                                                                              <  1:618874‑M2/56‑5 (MQ=255)                                            gtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCatatat                                                                                                                                                                                                                         >  1:497351‑M2/192‑204 (MQ=255)                                              ttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAtt                                                                                                                                                                       <  1:845801‑M2/62‑1 (MQ=255)                                                gcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGgttatac                                                                                                                                                                                                                 >  1:482943‑M2/188‑205 (MQ=255)                                                  atttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                   <  1:761325‑M2/66‑1 (MQ=255)                                                      actcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTAtt                                                                                                                                                               >  1:66273‑M2/182‑251 (MQ=255)                                                         cggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCaggcttttaaggttgctgcacggaagggaccggat                                                                                                                                                                      >  1:333148‑M2/179‑224 (MQ=255)                                                          ggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCa                                                                                                                                                           >  1:472576‑M2/178‑251 (MQ=255)                                                           gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGccatatatgg                                                                                                                                                                                                                       >  1:621619‑M2/177‑189 (MQ=255)                                                           gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCagg                                                                                                                                                                                                      >  1:610940‑M2/177‑206 (MQ=255)                                                           gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCtgc                                                                                                                                                                                      <  1:127641‑M2/47‑2 (MQ=255)                                                           gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAt                                                                                                                                                                        >  1:150714‑M2/177‑237 (MQ=255)                                                           gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                   <  1:855640‑M2/66‑1 (MQ=255)                                                                gatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGa                                                                                                                                                     >  1:471959‑M2/172‑251 (MQ=255)                                                                 atcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGt                                                                                                                                                                                                              <  1:349224‑M2/23‑1 (MQ=255)                                                                 atcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGaa                                                                                                                                                    >  1:446602‑M2/171‑251 (MQ=255)                                                                    atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                 >  1:722156‑M2/168‑251 (MQ=255)                                                                    atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                 >  1:690648‑M2/168‑251 (MQ=255)                                                                      attgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCatatat                                                                                                                                                                                                                         <  1:336420‑M2/13‑1 (MQ=255)                                                                      attgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGt                                                                                                                                                                                                              >  1:181142‑M2/166‑188 (MQ=255)                                                                       ttgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAata                                                                                                                                              <  1:426788‑M2/87‑1 (MQ=255)                                                                         gaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAgccatatatggt                                                                                                                                                                                                                      >  1:780432‑M2/163‑175 (MQ=255)                                                                             atgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGt                                                                                                                                        <  1:842769‑M2/93‑1 (MQ=255)                                                                              tgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                             >  1:204574‑M2/158‑245 (MQ=255)                                                                                         aggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtac                                                                                                                             <  1:702070‑M2/104‑1 (MQ=255)                                                                                          ggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                             <  1:333155‑M2/88‑1 (MQ=255)                                                                                            aaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtacttac                                                                                                                         <  1:187543‑M2/108‑1 (MQ=255)                                                                                                                  aattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGt                                                                                                                                                                                                              >  1:658073‑M2/122‑144 (MQ=255)                                                                                                                    ttatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAgccatatatggt                                                                                                                                                                                                                      >  1:383742‑M2/120‑132 (MQ=255)                                                                                                                    ttatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAgg                                                                                                                                                                                             >  1:366629‑M2/120‑159 (MQ=255)                                                                                                                                 cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                  <  1:189979/250‑1 (MQ=255)                                                                                                                                       cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:386685/251‑1 (MQ=255)                                                                                                                                        gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:43973/250‑1 (MQ=255)                                                                                                                                         aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCt                                                                                                                                                                  <  1:460939/162‑1 (MQ=255)                                                                                                                                         aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAgg                                                                                                                                                  <  1:391927/178‑1 (MQ=255)                                                                                                                                            atccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatc                                                                            >  1:444596‑M2/96‑248 (MQ=255)                                                                                                                                            atccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                  <  1:580545‑M2/147‑1 (MQ=255)                                                                                                                                             gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                                              >  1:42256/1‑210 (MQ=255)                                                                                                                                                 tatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCg                                                                    >  1:711569‑M2/91‑251 (MQ=255)                                                                                                                                                          ggagatgtgtataagaaaaattgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCA‑‑TA‑A‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATTTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAAcc                                                         <  1:522820/229‑1 (MQ=255)                                                                                                                                                                  aaaaGTCGGAAAGTGAAAAACTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATA‑CGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                <  1:223172/251‑1 (MQ=255)                                                                                                                                                                          gaaagtgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAat                                                                                                                                               <  1:424091/144‑1 (MQ=255)                                                                                                                                                                                 tAAAACTATATCGAAAAAACAGGGCCAACGGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATA‑CGTAATCCAGGTTTTTAGGGTGGCGCCAGCGGAACAATGATTATTCTATTTTCACCCTAAAGGAATATCATTTTAAAAAAATACTTGTTTATTTTACCGTTTTGTATTTAAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcccttccgtgc                                 <  1:508630/250‑14 (MQ=255)                                                                                                                                                                                 gtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAAc                                                                                                <  1:771184‑M2/133‑1 (MQ=255)                                                                                                                                                                                 gtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTatcc‑ggtcc                                          <  1:396556‑M2/186‑5 (MQ=255)                                                                                                                                                                                          ttCGGAAAAACAGGTTAAAACGGGAATTCTTGCTTCATTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATA‑CGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTAAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:810714/251‑1 (MQ=255)                                                                                                                                                                                          ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:710923/251‑1 (MQ=255)                                                                                                                                                                                              gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:143318/239‑1 (MQ=255)                                                                                                                                                                                                      gggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:330856/188‑1 (MQ=255)                                                                                                                                                                                                        gTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATg         >  1:327642/1‑251 (MQ=255)                                                                                                                                                                                                               gggCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGtttt  >  1:153639/1‑251 (MQ=255)                                                                                                                                                                                                                          tcttgcttcattttccattaaaaGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTGTTATTTTAGCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATaa                          <  1:258853/201‑1 (MQ=255)                                                                                                                                                                                                                                          attaaaaGCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATc                                                                                                                                            <  1:338663/88‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       >  1:593899/1‑201 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                            CCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACA‑CGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTT  >  1/727519‑727980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑