Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	727,531	T→C	intergenic (‑4817/‑59660)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	727,531	0	T	C	100.0%	209.4 / NA	70	intergenic (‑4817/‑59660)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

ACAATGGGCAACAGCGGCAGTGCAACCGCAAAGCGGGGAAATTGAGATGACCGTACGCATTGTGGACGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACAT  >  1/727287‑727765                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gcAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                                                                                                                                                                                                      >  1:174269/2‑251 (MQ=255)    aTGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGca                                                                                                                                                                                                                                    >  1:797760/1‑250 (MQ=255)         cAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTACCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATCTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtg                                                                                                                                                                                                                              >  1:216902/1‑251 (MQ=255)              gCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                                                                                                                                                                          <  1:141467/250‑1 (MQ=255)                     tGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCac                                                                                                                                                                                                                     <  1:876441/248‑2 (MQ=255)                         accgcaaagtgatgaagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGtt                                                                                                                                                                                                              >  1:67083/23‑251 (MQ=255)                                   gatgaagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                                                                                                                                                                                                               >  1:674036/13‑224 (MQ=255)                                       aagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                                                                                                                                                                                                      <  1:761752/205‑1 (MQ=255)                                         gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGACCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTa                                                                                                                                                                                              >  1:782267/7‑251 (MQ=255)                                         gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAACGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTc                                                                                                                                                                                                   >  1:576827/7‑246 (MQ=255)                                          tggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                                                                                                                                                                          <  1:147480/217‑1 (MQ=255)                                           ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                                                                                                                                                                                                               <  1:863527/212‑1 (MQ=255)                                           ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATg                                                                                                                                                                                            >  1:769070/5‑251 (MQ=255)                                             aaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGAt                                                                                                                                                                                          >  1:141228/3‑251 (MQ=255)                                              aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         <  1:662514/250‑1 (MQ=255)                                              aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         <  1:318178/250‑1 (MQ=255)                                              aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         <  1:646851/250‑1 (MQ=255)                                                tgactgtgcGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCat                                                                                                                                                                                       >  1:726057/9‑251 (MQ=255)                                                            ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                                                                                                                                                                          >  1:349286/1‑204 (MQ=255)                                                            ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGa                                                                                                                                                                            >  1:196969/1‑250 (MQ=255)                                                              gtgGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         >  1:760957/1‑235 (MQ=255)                                                               tgGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGa                                                                                                                                                                         <  1:440046/250‑1 (MQ=255)                                                                ggACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAg                                                                                                                                                                       >  1:676804/1‑251 (MQ=255)                                                                      gatggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTc                                                                                                                                                                                                   <  1:778042/214‑1 (MQ=255)                                                                       atggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                                                                                                                                                                          <  1:298273/191‑1 (MQ=255)                                                                       atggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCggagga                                                                                                                                                                >  1:894455/3‑251 (MQ=255)                                                                               ccACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGaa                                                                                                                                                         <  1:588389/250‑1 (MQ=255)                                                                               ccACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGaa                                                                                                                                                         <  1:189549/250‑1 (MQ=255)                                                                                    cATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         >  1:365236/1‑213 (MQ=255)                                                                                            ctcaaTTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCa                                                                                                                                           >  1:390134/4‑251 (MQ=255)                                                                                              caaTTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGAc                                                                                                                                                                           <  1:871402/216‑1 (MQ=255)                                                                                                     cGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGGAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                  <  1:3532/251‑1 (MQ=255)                                                                                                        ggCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAgg                                                                                                                               >  1:576486/1‑251 (MQ=255)                                                                                                             aGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCac                                                                                                                                                                                                                     <  1:81299/160‑2 (MQ=255)                                                                                                                   ccccACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGgaaa                                                                                                                                               >  1:831430/1‑222 (MQ=255)                                                                                                                   ccccACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTg                                                                                                                    >  1:59905/1‑251 (MQ=255)                                                                                                                     ccACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCACATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGt                                                                                                                   >  1:85136/1‑250 (MQ=255)                                                                                                                     ccACTAATGTGCTGTCTTTCCCTTTTGAATCCCCTGATGAAGTGGAACTGCCGTTATTGGGCAATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTc                                                                                                                  <  1:350772/251‑1 (MQ=255)                                                                                                                        cTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                                                                                                                                                                         <  1:510259/177‑1 (MQ=255)                                                                                                                             gtgCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTg                                                                                                                                                                                   <  1:120992/178‑1 (MQ=255)                                                                                                                                cTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATc                                                                                                        <  1:897916/250‑1 (MQ=255)                                                                                                                                cTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATc                                                                                                        <  1:210376/250‑1 (MQ=255)                                                                                                                                  gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                     >  1:40111/1‑251 (MQ=255)                                                                                                                                  gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                     >  1:521505/1‑251 (MQ=255)                                                                                                                                  gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                     >  1:630872/1‑251 (MQ=255)                                                                                                                                   tCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAg                                                                                                    <  1:588628/251‑1 (MQ=255)                                                                                                                                              tGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCt                                                                                                       >  1:793496/1‑237 (MQ=255)                                                                                                                                                 aTGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGaaa                                                                                      <  1:865091/251‑1 (MQ=255)                                                                                                                                                   gCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGggcttggc                                                                                                                        <  1:302764/215‑1 (MQ=255)                                                                                                                                                      cTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                  <  1:62584/202‑1 (MQ=255)                                                                                                                                                        gatgaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAg                                                                                                                                                                                >  1:422596/1‑154 (MQ=255)                                                                                                                                                        gatgaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCgg                                                                               >  1:867279/1‑251 (MQ=255)                                                                                                                                                        gatgaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCgg                                                                               >  1:354887/1‑251 (MQ=255)                                                                                                                                                        gaggaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATc                                                                                                              <  1:211345/217‑1 (MQ=255)                                                                                                                                                                    ctgccgttATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAAc                                                                   <  1:200994/244‑1 (MQ=255)                                                                                                                                                                       ccgttATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGAGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCAGTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTa                                                                 <  1:433959/246‑1 (MQ=255)                                                                                                                                                                                           gATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGaaag                                                                           <  1:28042/220‑3 (MQ=255)                                                                                                                                                                                                gtcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                  >  1:887233/3‑160 (MQ=255)                                                                                                                                                                                                gtcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACggg                                        >  1:542021/3‑250 (MQ=255)                                                                                                                                                                                                 tcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCa                                      <  1:9290/250‑1 (MQ=255)                                                                                                                                                                                                        gtggtgGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTg                                >  1:143813/1‑250 (MQ=255)                                                                                                                                                                                                                      ggctcaagagcaagagaaacCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTa                 >  1:148120‑M1/5‑250 (MQ=255)                                                                                                                                                                                                                       gctcaagagcaagagaaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaa                >  1:606047‑M1/4‑249 (MQ=255)                                                                                                                                                                                                                       gctcaagagcaagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGaa                                                         <  1:808703/207‑1 (MQ=255)                                                                                                                                                                                                                             gagCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACg                                          <  1:353159/219‑1 (MQ=255)                                                                                                                                                                                                                             gagCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGttg                       <  1:817617/238‑3 (MQ=255)                                                                                                                                                                                                                                cAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat       >  1:653899‑M1/1‑240 (MQ=255)                                                                                                                                                                                                                                 aaGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatata      <  1:139343‑M1/251‑13 (MQ=255)                                                                                                                                                                                                                                      aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt  <  1:591276‑M1/250‑17 (MQ=255)                                                                                                                                                                                                                                      aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatgg   <  1:411858‑M1/249‑16 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           ACAATGGGCAACAGCGGCAGTGCAACCGCAAAGCGGGGAAATTGAGATGACCGTACGCATTGTGGACGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACAT  >  1/727287‑727765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑