Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	727,380	T→C	intergenic (‑4666/‑59811)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	727,380	0	T	C	100.0%	228.6 / NA	75	intergenic (‑4666/‑59811)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/32);  total (43/32)

GGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATTGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGCGCAAATTGAACAATGGGCAACAGCGGCAGTGCAACCGCAAAGCGGGGAAATTGAGATGACCGTACGCATTGTGGACGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAA  >  1/727135‑727614                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                                                       >  1:4973/1‑251 (MQ=255) gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                                                       >  1:458737/1‑251 (MQ=255)   gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTAt                                                                                                                                                                                                                                      >  1:823374/1‑250 (MQ=255)            atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAg                                                                                                                                                                                                                            >  1:526905/1‑251 (MQ=255)              ccTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAGAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                                                                                                                                                                          >  1:430183/1‑251 (MQ=255)                 atatatTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                                                       >  1:231436/1‑235 (MQ=255)                 atatatTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                                                                                                                                                                        >  1:514291/1‑250 (MQ=255)                 atatatTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                                                                                                                                                                        >  1:210651/1‑250 (MQ=255)                      ttGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCa                                                                                                                                                                                                                   >  1:583102/1‑250 (MQ=255)                         cTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                                                                                                                                                                               <  1:866114/251‑1 (MQ=255)                            gAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                                                             <  1:533243/250‑1 (MQ=255)                            gAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                                                             <  1:310007/250‑1 (MQ=255)                                gtcgtGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCg                                                                                                                                                                                                                                    >  1:549858/1‑223 (MQ=255)                                gtcgtGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                                                                                                                                                                          >  1:896642/1‑233 (MQ=255)                                gtcgtGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                                                             >  1:851079/1‑246 (MQ=255)                                     gCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCttt                                                                                                                                                                                                   >  1:897207/1‑251 (MQ=255)                                             cGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTg                                                                                                                                                                                           <  1:164981/251‑1 (MQ=255)                                                            aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                                                                                                                                                                            <  1:888932/251‑1 (MQ=255)                                                            aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                                                                                                                                                                            <  1:545251/251‑1 (MQ=255)                                                              gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                                                             >  1:195714/1‑216 (MQ=255)                                                              gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGt                                                                                                                                                                           <  1:382044/250‑1 (MQ=255)                                                               aaTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATCCCCTGATGAAGTgg                                                                                                                                                                         >  1:770484/1‑251 (MQ=255)                                                                aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTg                                                                                                                                                                          <  1:127274/249‑1 (MQ=255)                                                                aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                                                                                                                                                                        >  1:643511/1‑251 (MQ=255)                                                                aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                                                                                                                                                                        >  1:462168/1‑251 (MQ=255)                                                                   tATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGaa                                                                                                                                                                       >  1:764983/1‑248 (MQ=255)                                                                                 gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGc                                                                                                                                                        >  1:121083/1‑250 (MQ=255)                                                                                 gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCg                                                                                                                                                       >  1:282504/1‑251 (MQ=255)                                                                                  ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCtgatga                                                                                                                                                                              >  1:80159/1‑227 (MQ=255)                                                                                  ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGa                                                                                                                                                      >  1:2257/1‑251 (MQ=255)                                                                                     aaaaTGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGAttt                                                                                                                                                   <  1:843914/251‑1 (MQ=255)                                                                                          gATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                                                                                                                                                                               <  1:884109/186‑1 (MQ=255)                                                                                          gATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGa                                                                                                                                              >  1:275563/1‑251 (MQ=255)                                                                                           attattGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGAt                                                                                                                                             >  1:190239/1‑251 (MQ=255)                                                                                                   tttACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTgtcgtc                                                                                                                                     <  1:546676/251‑1 (MQ=255)                                                                                                                      gATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAg                                                                                                                                  <  1:166424/235‑1 (MQ=255)                                                                                                                         gAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGc                                                                                                                                                                                      >  1:279376/1‑180 (MQ=255)                                                                                                                               ggCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTccc                                                                                                                                                                                                <  1:673872/164‑1 (MQ=255)                                                                                                                                        aCGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAAcc                                                                                                <  1:395898/251‑1 (MQ=255)                                                                                                                                                  aaaTTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTccc                                                                                                                                                                                                <  1:405567/145‑1 (MQ=255)                                                                                                                                                         gcAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                                               >  1:174269/2‑251 (MQ=255)                                                                                                                                                            aTGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGca                                                                             >  1:797760/1‑250 (MQ=255)                                                                                                                                                               ggCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAg                                                                                                                          <  1:313794/202‑1 (MQ=255)                                                                                                                                                                 cAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTACCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATCTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtg                                                                       >  1:216902/1‑251 (MQ=255)                                                                                                                                                                      gCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                   <  1:141467/250‑1 (MQ=255)                                                                                                                                                                          cAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGag                                                                                                     >  1:691261/1‑211 (MQ=255)                                                                                                                                                                             tGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCac                                                              <  1:876441/248‑2 (MQ=255)                                                                                                                                                                                 accgcaaagtgatgaagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGtt                                                       >  1:67083/23‑251 (MQ=255)                                                                                                                                                                                           gatgaagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                                                        >  1:674036/13‑224 (MQ=255)                                                                                                                                                                                               aagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                                               <  1:761752/205‑1 (MQ=255)                                                                                                                                                                                                 gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGACCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTa                                       >  1:782267/7‑251 (MQ=255)                                                                                                                                                                                                 gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAACGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTc                                            >  1:576827/7‑246 (MQ=255)                                                                                                                                                                                                  tggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                   <  1:147480/217‑1 (MQ=255)                                                                                                                                                                                                   ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGag                                                                                                     <  1:384011/183‑2 (MQ=255)                                                                                                                                                                                                   ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                                                        <  1:863527/212‑1 (MQ=255)                                                                                                                                                                                                   ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATg                                     >  1:769070/5‑251 (MQ=255)                                                                                                                                                                                                     aaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGAt                                   >  1:141228/3‑251 (MQ=255)                                                                                                                                                                                                      aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                  <  1:662514/250‑1 (MQ=255)                                                                                                                                                                                                      aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                  <  1:646851/250‑1 (MQ=255)                                                                                                                                                                                                      aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                  <  1:318178/250‑1 (MQ=255)                                                                                                                                                                                                        tgactgtgcGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCat                                >  1:726057/9‑251 (MQ=255)                                                                                                                                                                                                          actgtgcGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGtggtgg                                                                                                                            <  1:328432/151‑1 (MQ=255)                                                                                                                                                                                                                    ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAagag                                                                                                           >  1:533413/1‑164 (MQ=255)                                                                                                                                                                                                                    ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                   >  1:349286/1‑204 (MQ=255)                                                                                                                                                                                                                    ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGa                     >  1:196969/1‑250 (MQ=255)                                                                                                                                                                                                                    ttGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCACCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGtggtgg                                                                                                                            <  1:644194/147‑1 (MQ=255)                                                                                                                                                                                                                      gtgGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                  >  1:760957/1‑235 (MQ=255)                                                                                                                                                                                                                       tgGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGa                  <  1:440046/250‑1 (MQ=255)                                                                                                                                                                                                                        ggACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAg                >  1:676804/1‑251 (MQ=255)                                                                                                                                                                                                                              gatggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTc                                            <  1:778042/214‑1 (MQ=255)                                                                                                                                                                                                                               atggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                                                   <  1:298273/191‑1 (MQ=255)                                                                                                                                                                                                                               atggAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCggagga         >  1:894455/3‑251 (MQ=255)                                                                                                                                                                                                                                       ccACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGaa  <  1:189549/250‑1 (MQ=255)                                                                                                                                                                                                                                       ccACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGaa  <  1:588389/250‑1 (MQ=255)                                                                                                                                                                                                                                            cATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc                                  >  1:365236/1‑213 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           GGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATTGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGCGCAAATTGAACAATGGGCAACAGCGGCAGTGCAACCGCAAAGCGGGGAAATTGAGATGACCGTACGCATTGTGGACGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAA  >  1/727135‑727614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑