Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	63,992	T→C	intergenic (+59385/+66513)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	63,992	0	T	C	100.0%	124.6 / NA	42	intergenic (+59385/+66513)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/25);  total (17/25)

TTTGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATATTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAG  >  1/63761‑64233                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  tttGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGc                                                                                                                                                                                                                                <  1:854603/251‑1 (MQ=255)          ctGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCaa                                                                                                                                                                                                                       <  1:891994/251‑1 (MQ=255)            gCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGCTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATg                                                                                                                                                                                                                     >  1:885140/1‑251 (MQ=255)                      tAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATggggg                                                                                                                                                                                                           <  1:117277/251‑1 (MQ=255)                                 aaaaaTTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:444166/251‑1 (MQ=255)                                                       ctTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGc                                                                                                                                                                                                                                           <  1:533396/186‑1 (MQ=255)                                                                tATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGgtg                                                                                                                                                                 <  1:678377/251‑1 (MQ=255)                                                                      gttgttATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAagag                                                                                                                                                                                      >  1:100388/1‑224 (MQ=255)                                                                              tCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATaa                                                                                                                                                                                         <  1:619806/213‑1 (MQ=255)                                                                                                              aaataaTAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttg                                                                                                                                                                                                                                      <  1:206348/136‑1 (MQ=255)                                                                                                                      atGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:501345/166‑1 (MQ=255)                                                                                                                      atGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCaa                                                                                                           >  1:568026/1‑251 (MQ=255)                                                                                                                        gTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttgttg                                                                                                                                                                                                                                   >  1:336208/1‑129 (MQ=255)                                                                                                                        gTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAAccc                                                                                                                                                    <  1:587504/208‑1 (MQ=255)                                                                                                                         ttAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAAcc                                                                                                         <  1:561093/250‑1 (MQ=255)                                                                                                                              ttttGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGc                                                                                                                                                                                                                         <  1:13931/133‑1 (MQ=255)                                                                                                                                     aCATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:847543/1‑156 (MQ=255)                                                                                                                                     aCATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGc                                                                                                                                              <  1:791441/201‑1 (MQ=255)                                                                                                                                          gCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGgtg                                                                                                                                                                 <  1:855122/177‑1 (MQ=255)                                                                                                                                          gCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGAt                                                                                       >  1:894963/1‑251 (MQ=255)                                                                                                                                                               gATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:806521/1‑130 (MQ=255)                                                                                                                                                                     gCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttg                                                                                                                                                                                                                                      <  1:874443/81‑1 (MQ=255)                                                                                                                                                                     gCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTttg                                                            >  1:731297/1‑251 (MQ=255)                                                                                                                                                                        gAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttgt                                                                                                                                                                                                                                     >  1:180119/1‑79 (MQ=255)                                                                                                                                                                        gAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:901712/1‑121 (MQ=255)                                                                                                                                                                                 ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                >  1:897904/1‑251 (MQ=255)                                                                                                                                                                                 ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                <  1:543238/251‑1 (MQ=255)                                                                                                                                                                                 ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                >  1:302992/1‑251 (MQ=255)                                                                                                                                                                                   gTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAagag                                                                                                                                                                                      <  1:124031/115‑1 (MQ=255)                                                                                                                                                                                           aacaaACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGc                                      <  1:192310/251‑1 (MQ=255)                                                                                                                                                                                               aaCTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCa                                                                                                                                                                                                                 <  1:781939/76‑1 (MQ=255)                                                                                                                                                                                                     attattTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTttgttg                                                         <  1:335062/222‑1 (MQ=255)                                                                                                                                                                                                        attTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAAcc                                                                                                         >  1:571820/1‑171 (MQ=255)                                                                                                                                                                                                                  aTACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGTCCTATGgtgt                                                                                                                                                                >  1:593947/1‑106 (MQ=255)                                                                                                                                                                                                                    aCCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:624210/72‑1 (MQ=255)                                                                                                                                                                                                                           caaacaaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGc                                      <  1:223150/219‑1 (MQ=255)                                                                                                                                                                                                                               caaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAGCCAGCCAAAGTGCGGTCGATTTTACGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAg  >  1:443710/1‑251 (MQ=255)                                                                                                                                                                                                                               caaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAg  >  1:504922/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATgg                                                                                                                                                                   >  1:695820/1‑87 (MQ=255)                                                                                                                                                                                                                                  aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAAt                                             <  1:52247/205‑1 (MQ=255)                                                                                                                                                                                                                                  aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTc                                 <  1:349380/217‑1 (MQ=255)                                                                                                                                                                                                                                  aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCgg                          <  1:865164/224‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TTTGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATATTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAG  >  1/63761‑64233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑