Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	675,615	T→C	intergenic (+65441/+17742)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	675,615	0	T	C	100.0%	208.1 / NA	69	intergenic (+65441/+17742)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

GATCAAATCAGCGCCTTGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGTTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGACGATTGGAAGGAAAACGCAG  >  1/675414‑675861                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gATCAAATCAGCGCCTTGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTgg                                                                                                                                                                                                        >  1:474553/1‑250 (MQ=255)      aaTCAGCGCCTTGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAgg                                                                                                                                                                                                  >  1:796717/1‑251 (MQ=255)               cTTGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAgcg                                                                                                                                                                                         <  1:735764/251‑1 (MQ=255)                ttGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAgcgc                                                                                                                                                                                        <  1:656903/251‑1 (MQ=255)                  gAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAgc                                                                                                                                                                                          <  1:604789/247‑1 (MQ=255)                   aaTCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCAt                                                                                                                                                                                     >  1:260571/1‑251 (MQ=255)                    aTCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCAtt                                                                                                                                                                                    >  1:110150/1‑251 (MQ=255)                        gAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGt                                                                                                                                                                                                                                                 >  1:328662/1‑186 (MQ=255)                          aTTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCTGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGcc                                                                                                                                                                              <  1:455346/251‑1 (MQ=255)                                    cAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAAc                                                                                                                                                                    <  1:775849/251‑1 (MQ=255)                                    cAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAAc                                                                                                                                                                    <  1:610204/251‑1 (MQ=255)                                                ccTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTc                                                                                                                                                          <  1:436265/249‑1 (MQ=255)                                                     cGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAAc                                                                                                                                                   <  1:429530/251‑1 (MQ=255)                                                     cGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAAc                                                                                                                                                   <  1:276241/251‑1 (MQ=255)                                                     cGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAAc                                                                                                                                                   <  1:28005/251‑1 (MQ=255)                                                     cGGCATGGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAAc                                                                                                                                                   <  1:277903/251‑1 (MQ=255)                                                             caacaaCAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGa                                                                                                                                           >  1:121442/1‑251 (MQ=255)                                                                         cAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGATTGGCTGCTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAATTGGTGTTGGATAATGACGTGGATACCAGCGTGTCCCTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCGCAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAATTGTTGTCCCTTAACAATGTGGATCAAAACGCcat                                                                                                                               <  1:543955/251‑1 (MQ=255)                                                                                   ccAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAAt                                                                                                                     <  1:310727/251‑1 (MQ=255)                                                                                     aaTCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCCGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAAttt                                                                                                                   >  1:437239/1‑251 (MQ=255)                                                                                      aTCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAAc                                                                                                                                                   >  1:635722/1‑218 (MQ=255)                                                                                          aGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATcc                                                                                                               >  1:264994/1‑250 (MQ=255)                                                                                          aGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATcc                                                                                                               >  1:69277/1‑250 (MQ=255)                                                                                               aaGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGt                                                                                                                                                                                                          >  1:62699/1‑154 (MQ=255)                                                                                                          ccGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAAt                                                                                                                                    <  1:555961/213‑1 (MQ=255)                                                                                                               tGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATg                                                                                         <  1:724336/251‑1 (MQ=255)                                                                                                                gACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTg                                                                                                                                                                                                >  1:638981/1‑147 (MQ=255)                                                                                                                 aCTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAgc                                                                                                                                                                                          >  1:299429/1‑152 (MQ=255)                                                                                                                 aCTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGaa                                                                                       >  1:399412/1‑251 (MQ=255)                                                                                                                 aCTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGaa                                                                                       >  1:885435/1‑251 (MQ=255)                                                                                                                     gTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGa                                                                                   >  1:294737/1‑251 (MQ=255)                                                                                                                        aCTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACgg                                                                                >  1:47828/1‑251 (MQ=255)                                                                                                                        aCTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACgg                                                                                <  1:111239/251‑1 (MQ=255)                                                                                                                           gACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAAttt                                                                                                                   >  1:861762/1‑213 (MQ=255)                                                                                                                                aaGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGAt                                                                        <  1:101244/251‑1 (MQ=255)                                                                                                                                aaGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGAt                                                                        <  1:833366/251‑1 (MQ=255)                                                                                                                                  gCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGc                                                                                                                          <  1:187437/199‑1 (MQ=255)                                                                                                                                   cGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGAtg                                                                       <  1:189532/249‑1 (MQ=255)                                                                                                                                             gTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTtgatg                                                           >  1:476474/1‑251 (MQ=255)                                                                                                                                                aaaTAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGAt                                                                                          <  1:486131/217‑1 (MQ=255)                                                                                                                                                 aaTAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAgttg                                                                                                                                                               >  1:772636/1‑147 (MQ=255)                                                                                                                                                     aCGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCg                                                   <  1:479271/251‑1 (MQ=255)                                                                                                                                                        cTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAg                                                 <  1:859701/250‑1 (MQ=255)                                                                                                                                                            gcgcAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATcc                                                                                                               >  1:855578/1‑184 (MQ=255)                                                                                                                                                            gcgcAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACgg                                                                                >  1:418387/1‑215 (MQ=255)                                                                                                                                                            gcgcAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCg                                            >  1:144317/1‑251 (MQ=255)                                                                                                                                                                   cTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGtt                                     >  1:224902/1‑251 (MQ=255)                                                                                                                                                                      gtgtTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGAt                                                                        >  1:441710/1‑213 (MQ=255)                                                                                                                                                                      gtgtTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGa                                   >  1:147107/1‑250 (MQ=255)                                                                                                                                                                      gtgtTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGa                                   >  1:359259/1‑250 (MQ=255)                                                                                                                                                                       tgtTGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGAc                                  <  1:174803/250‑1 (MQ=255)                                                                                                                                                                         ttGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACgg                                <  1:294495/250‑1 (MQ=255)                                                                                                                                                                         ttGGATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGa                               <  1:310208/251‑1 (MQ=255)                                                                                                                                                                           ggATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGAcc                                                                                                                                         <  1:132231/143‑1 (MQ=255)                                                                                                                                                                           ggATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCaa                                                                                                                                       >  1:176705/1‑145 (MQ=255)                                                                                                                                                                           ggATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGAtt                             >  1:631449/1‑251 (MQ=255)                                                                                                                                                                           ggATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGAtt                             >  1:338331/1‑251 (MQ=255)                                                                                                                                                                            gATAACGACGTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGt                                                <  1:579145/231‑1 (MQ=255)                                                                                                                                                                                 cgacgTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAg                       >  1:861344/1‑251 (MQ=255)                                                                                                                                                                                   acgTGGATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGa                      <  1:731325/250‑1 (MQ=255)                                                                                                                                                                                       ggATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACgg                                                                                >  1:728720/1‑188 (MQ=255)                                                                                                                                                                                       ggATACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGa                      <  1:739616/246‑1 (MQ=255)                                                                                                                                                                                         aTACCAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGt                                                                                                                                                                                                          >  1:893108/1‑64 (MQ=255)                                                                                                                                                                                            ccAGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTg                                                                                                                                                                                                <  1:511330/71‑1 (MQ=255)                                                                                                                                                                                              aGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAAc                                                                                                                                                                    <  1:774550/97‑1 (MQ=255)                                                                                                                                                                                              aGTGTGTCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGACGATTggaagga          >  1:496632/1‑251 (MQ=255)                                                                                                                                                                                               gtgtgtCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGACGATTggaaggaa         >  1:618938/1‑251 (MQ=255)                                                                                                                                                                                                 gtgtCCTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACgg                                                                                <  1:835579/178‑1 (MQ=255)                                                                                                                                                                                                      cTTGCTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGACGATTGGAAGGAAAACGCag  >  1:721730/1‑251 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GATCAAATCAGCGCCTTGAATCAGAATTTGTCCGCCAAAGAACAAAGCCTGTCGGCATTGCAACAACAGGTGCAACGTTTGTCCAATCAAGCCAAAGCGGAGCAGCCGAATGACTGGTTACTGACCGAAGCGGATTTTCTGTTAAATAACGCTTTGCGCAAACTGGTGTTGGATAACGACGTGGATACCAGTGTGTCCTTGTTGAAAGTTGCCGATGAAACCCTTTCCAAAGTCGCCATGCCACAAGTGGCGCAGGTGCGTAGCGCCATTAACGCCGATTTAAAACAGTTGTTGTCCCTGAACAATGTGGACCAAAATGCCATCATGCAGAATTTATCCCAGCTGGCGAATAACGTAGATGAATTGACGGTGCTGGATGTGAATTTTGATGAAGATCCGAGTAGCGACAAGTTGACGGATTCCTTAGACGATTGGAAGGAAAACGCAG  >  1/675414‑675861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑