Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	666,327	G→A	intergenic (+56153/+27030)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	666,327	0	G	A	100.0%	210.6 / NA	72	intergenic (+56153/+27030)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/24);  total (48/24)

CTGACCGCTAATTTGTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATGGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCAA  >  1/666097‑666573                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       cccgccgttatttggTTCGGCGTTGTTGTTTTCGCTTTTAACGGGGGTTTGGTTCGGGCGC‑TGTTGTGGGAAGGAAAAGCGGGTGGGGGTAAAA‑C‑CCGCATGAATGCTTGGGGGGGCGATGCTGCTTTTTATTTTTACGTTCCTTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTAt                                                                                                                                                                                                                                    <  1:491240/237‑1 (MQ=255)     ccGCTAATTTGTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGt                                                                                                                                                                                                                                 >  1:719162/1‑250 (MQ=255)          aaTTTGTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCAt                                                                                                                                                                                                                           >  1:349206/1‑251 (MQ=255)           aTTTGTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTATAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATa                                                                                                                                                                                                                          >  1:453277/1‑251 (MQ=255)               gTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGCTAAAATCCGATGTATCACCGAGATAGCCCTGc                                                                                                                                                                                                                                                 >  1:385964/1‑224 (MQ=255)                       cTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATaa                                                                                                                                                                                                              >  1:214470/1‑251 (MQ=255)                        tgttgtTTTCGCTTTTAATAGGGGTTTGGTTGGGGCGGTTG‑TGTTGGAAGGAAAGGCGGGTTGGGTTTAAA‑C‑CCGAATGAATGTTTGGTGGGGGGATGCTGCTGTTTATTTTTACGTTGCCTTTAAAC‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAAc                                                                                                                                                                                                             <  1:527246/251‑1 (MQ=255)                                  gCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGatat                                                                                                                                                                                                   >  1:566063/1‑251 (MQ=255)                                   ccTTTAATCGGGGTTTGGTTCAGGCGCATG‑TGTGGGAATGCAAAGCGGTTTGCGGTTAAA‑T‑CCGCATGATTGCTTGGTGGGGCGATGCTGCTTTTTAATTTTACGTTGCTTTTCAAC‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTAt                                                                                                                                                                                                                                    <  1:337532/217‑1 (MQ=255)                                        aaTCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCa                                                                                                                                                                                             >  1:490719/1‑251 (MQ=255)                                             gggCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAActg                                                                                                                                                                                         >  1:475255/1‑250 (MQ=255)                                              ggtttggttggggcgtttg‑tgtgGGAATGCAAGGGGGTTTG‑GGTTAAA‑TCCCGAATAATTGCTGGGTCGGGCGTTGCTGCTTTTTATTTTTACGTTGCTTTACAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAActgc                                                                                                                                                                                        <  1:75555/233‑1 (MQ=255)                                              ggCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAActg                                                                                                                                                                                         >  1:734972/1‑249 (MQ=255)                                                 cgggttcgggctcttg‑tgTTGAAATGCAAGGCGGTTTGC‑GTTAAACC‑CCGCATAAATGCTGGGTGGGGCGTTGCTGCCTTTTATTTTTACGTTGCTTTAAAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGAt                                                                                                                                                                                    <  1:360750/237‑1 (MQ=255)                                                    gTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTg                                                                                                                                                                                  >  1:239527/1‑250 (MQ=255)                                                    gTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTg                                                                                                                                                                                  >  1:872960/1‑250 (MQ=255)                                                         ggcgtgtg‑tgtgGGAATGAAAGGCGGTTTGCGGTAAAA‑C‑CCGAATGAATGGTTGGGGGGGCGATGCTGCCTTTTATTTTTCCGTTCCCTTCCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGatat                                                                                                                                                                                                   <  1:463548/222‑1 (MQ=255)                                                         ggCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTg                                                                                                                                                                                                           >  1:5316/1‑220 (MQ=255)                                                          gcgcATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGTGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAg                                                                                                                                                                           >  1:283183/1‑251 (MQ=255)                                                          gcgcATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAg                                                                                                                                                                           >  1:570142/1‑251 (MQ=255)                                                             cATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGa                                                                                                                                                                        >  1:834135/1‑251 (MQ=255)                                                                g‑tgtTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGccc                                                                                                                                                                                                                                                    >  1:750139/1‑172 (MQ=255)                                                                g‑tgtTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAg                                                                                                                                                                                                                        >  1:387541/1‑200 (MQ=255)                                                                g‑tgtTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCaa                                                                                                                                                                                                                 >  1:800147/1‑207 (MQ=255)                                                                g‑tgtTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGAtt                                                                                                                                                                                   >  1:272088/1‑237 (MQ=255)                                                                g‑tgtTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAAt                                                                                                                                                                     >  1:90617/1‑251 (MQ=255)                                                                                          gTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAActgctg                                                                                                                                                                                      >  1:722612/1‑209 (MQ=255)                                                                                              aa‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGa                                                                                                                                         >  1:477503/1‑250 (MQ=255)                                                                                                   ccGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAgt                                                                                                                                     >  1:478852/1‑251 (MQ=255)                                                                                                      aaggattggttGGTGGGGCGTTGCTGCCTTTTATTTTTACGTT‑CCTTTTAAACCCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAgtgt                                                                                                                                   <  1:514220/241‑1 (MQ=255)                                                                                                                ttttCGGGCGATGCTGCCTTTTAATTTTACGTTGCCTTTCAAA‑CCCTCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTg                                                                                                                                                                                                                      >  1:3310/4‑157 (MQ=255)                                                                                                                      ggCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAg                                                                                                                                      >  1:692436/1‑231 (MQ=255)                                                                                                                        cGATGCTGCCTTTTAATTTTCCTTTCCCTTACAAC‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTc                                                                                                                 <  1:659334/250‑1 (MQ=255)                                                                                                                               gcCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAat                                                                                                                          >  1:25732/1‑234 (MQ=255)                                                                                                                                       aTTTTACGTTGCCTTTCAAA‑CCCGCCGATAACTCCGCCCACAAGCCCTGTAAATTCCGCGCATTAATGTCCAGGTTAAAATCCCATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTg                                                                                                  >  1:838119/1‑250 (MQ=255)                                                                                                                                       aTTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGAc                                                                                                                                        >  1:606204/1‑212 (MQ=255)                                                                                                                                           tACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCaa                                                                                              >  1:547968/1‑250 (MQ=255)                                                                                                                                                 gCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCGGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCttt                                                                                       >  1:204577/1‑251 (MQ=255)                                                                                                                                                  ccTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTg                                                                                                                             >  1:774399/1‑212 (MQ=255)                                                                                                                                                               gccgATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGc                                                                           >  1:181335/1‑250 (MQ=255)                                                                                                                                                                ccgATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTAt                                                                                                                                                                                                                                    <  1:25788/96‑1 (MQ=255)                                                                                                                                                                ccgATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGcc                                                                          <  1:784830/250‑1 (MQ=255)                                                                                                                                                                ccgATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGc                                                                           >  1:641284/1‑249 (MQ=255)                                                                                                                                                                       aTCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGc                                                                  <  1:41509/251‑1 (MQ=255)                                                                                                                                                                              cacaAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAAttt                                                           >  1:284632/1‑251 (MQ=255)                                                                                                                                                                                caAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGaa                                                                                                                           <  1:818111/185‑1 (MQ=255)                                                                                                                                                                                    cccTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGa                                                                    >  1:276892/1‑236 (MQ=255)                                                                                                                                                                                      cTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATa                                                   >  1:136347/1‑251 (MQ=255)                                                                                                                                                                                       tGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAat                                                                                                                          <  1:633795/179‑1 (MQ=255)                                                                                                                                                                                                   gcATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGcc                                                        <  1:566209/233‑1 (MQ=255)                                                                                                                                                                                                    cATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGc                                                                  <  1:358528/222‑1 (MQ=255)                                                                                                                                                                                                      ttAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGAt                                                                                                                                                                                    >  1:851519/1‑106 (MQ=255)                                                                                                                                                                                                      ttAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGc                                                                  >  1:610095/1‑220 (MQ=255)                                                                                                                                                                                                      ttAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGa                                    >  1:225661/1‑250 (MQ=255)                                                                                                                                                                                                      ttAATGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGa                                    >  1:458539/1‑250 (MQ=255)                                                                                                                                                                                                         aTGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGa                                <  1:755324/251‑1 (MQ=255)                                                                                                                                                                                                          tGTCCAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAAc                                                 <  1:36860/233‑1 (MQ=255)                                                                                                                                                                                                             ccAGGTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAg                            <  1:553818/251‑1 (MQ=255)                                                                                                                                                                                                                 gTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAg                            <  1:679100/247‑1 (MQ=255)                                                                                                                                                                                                                 gTTAAAATCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTAt                         <  1:13377/250‑1 (MQ=255)                                                                                                                                                                                                                     aaaTCCGATGTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGcc                    <  1:875234/251‑1 (MQ=255)                                                                                                                                                                                                                              gTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAAtt                                                            >  1:779697/1‑202 (MQ=255)                                                                                                                                                                                                                              gTATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGcc                    <  1:885381/242‑1 (MQ=255)                                                                                                                                                                                                                               tATCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGAtt          <  1:406478/251‑1 (MQ=255)                                                                                                                                                                                                                                aTCACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGAttt         >  1:25751/1‑251 (MQ=255)                                                                                                                                                                                                                                  cACCGAGATAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGAttttt       >  1:356025/1‑251 (MQ=255)                                                                                                                                                                                                                                      gagaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCa   >  1:792803/1‑251 (MQ=255)                                                                                                                                                                                                                                       agaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAAc                                                                                                                                                                                                             <  1:278632/48‑1 (MQ=255)                                                                                                                                                                                                                                       agaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCaa  >  1:144341/1‑251 (MQ=255)                                                                                                                                                                                                                                       agaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCaa  >  1:714580/1‑251 (MQ=255)                                                                                                                                                                                                                                       agaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCaa  >  1:216774/1‑251 (MQ=255)                                                                                                                                                                                                                                       agaTAGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCaa  >  1:653666/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CTGACCGCTAATTTGTTCCGCGCTGTTGATTTCGCCTTTAATCAGGGCTTGGTTCAGGCGCATG‑TGTTGGAATGCAAAGCGGCTTGCGGTTAAA‑T‑CCGCATGAATGCTTGGTCGGGCGATGCTGCCGTTTAATTTTACGTTGCCTTTCAAA‑CCCGCCGATAAATCCGCCCACAAGCCCTGTAAATTCGGCGCATTAATGTCCAGGTTAAAATCCGATGTATCACCGAGATGGCCCTGCACCTCAATTTTATTGTCGCCATAAGTGAGCAATAACTGCGGGATATTGAGCAACTGCTGATTGCCCGCAGTGAAATTGCCTTTCAGGTTCAGCGGGCGTTGGGACAGTGTGCCTTGAATATCCAATTCCGGCACGGCAACCTGCCAACCGCTTTGATTGGCTTTGCCTTGTGAGCTTAATTTGCCTGATAACTGGGCCGGGAAGACGGATAAGTATTTGCCCACATTGATTTTTGCCAA  >  1/666097‑666573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑