Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	664,438	G→A	intergenic (+54264/+28919)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	664,438	0	G	A	100.0%	216.9 / NA	72	intergenic (+54264/+28919)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

GCAATAATCGGTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTGTCGCCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAT  >  1/664197‑664673                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            gCAATAATCGGTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATcgtcg                                                                                                                                                                                                                                       >  1:899337/1‑245 (MQ=255)           gTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          >  1:766691/1‑251 (MQ=255)            tAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          <  1:865428/250‑1 (MQ=255)                   gagTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCg                                                                                                                                                                                                                  >  1:887244/1‑251 (MQ=255)                            cGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAata                                                                                                                                                                                                         >  1:864217/1‑251 (MQ=255)                                  aaCCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   <  1:249455/251‑1 (MQ=255)                                  aaCCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   <  1:245809/251‑1 (MQ=255)                                       tCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGc                                                                                                                                                                                              <  1:323667/251‑1 (MQ=255)                                         aaaTAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGc                                                                                                                                                                                              >  1:257654/1‑249 (MQ=255)                                            tAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         <  1:468933/251‑1 (MQ=255)                                              aCCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATcc                                                                                                                                                                                       <  1:117808/251‑1 (MQ=255)                                                   aCGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCgg                                                                                                                                                                                  <  1:368626/251‑1 (MQ=255)                                                         tATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTc                                                                                                                                                                             >  1:459341/1‑250 (MQ=255)                                                                 cTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         >  1:885871/1‑230 (MQ=255)                                                                          cgGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATgg                                                                                                                                                           <  1:600865/251‑1 (MQ=255)                                                                            gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAatat                                                                                                                                                                                                        <  1:611508/204‑1 (MQ=255)                                                                            gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATcc                                                                                                                                                                                       >  1:234796/1‑221 (MQ=255)                                                                            gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGtt                                                                                                                                                         >  1:195261/1‑251 (MQ=255)                                                                              gtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTa                                                                                                                                                        >  1:143414/1‑250 (MQ=255)                                                                                 aTGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACt                                                                                                                                                    <  1:723042/251‑1 (MQ=255)                                                                                              ccACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   >  1:116215/1‑191 (MQ=255)                                                                                              ccACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCg                                                                                                                                         >  1:203288/1‑249 (MQ=255)                                                                                                 ctactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCtata                                                                                                                                    >  1:339803/1‑251 (MQ=255)                                                                                                  tactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:372141/251‑1 (MQ=255)                                                                                                  tactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGAATCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:894009/251‑1 (MQ=255)                                                                                                   actTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATg                                                                                                                                                            <  1:466457/225‑1 (MQ=255)                                                                                                   actTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  <  1:884540/251‑1 (MQ=255)                                                                                                    ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTa                                                                                                                                                                                                              <  1:273812/174‑1 (MQ=255)                                                                                                    ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  >  1:367336/1‑250 (MQ=255)                                                                                                    ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCa                                                                                                                                 <  1:798283/251‑1 (MQ=255)                                                                                                     tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:81946/251‑1 (MQ=255)                                                                                                     tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:452445/251‑1 (MQ=255)                                                                                                     tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:223013/251‑1 (MQ=255)                                                                                                               tCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTcac                                                                                                                      <  1:222703/251‑1 (MQ=255)                                                                                                                  cccACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGTGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCacac                                                                                                                    <  1:461864/250‑1 (MQ=255)                                                                                                                  cccACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCacac                                                                                                                    <  1:206660/250‑1 (MQ=255)                                                                                                                      ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   >  1:386381/1‑231 (MQ=255)                                                                                                                      ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCa                                                                                                               >  1:644632/1‑251 (MQ=255)                                                                                                                      ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCa                                                                                                               >  1:467993/1‑251 (MQ=255)                                                                                                                           gcggTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         >  1:569850/1‑172 (MQ=255)                                                                                                                                     ggTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:609629/216‑1 (MQ=255)                                                                                                                                     ggTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGt                                                                                                  >  1:557757/1‑249 (MQ=255)                                                                                                                                      gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                >  1:383033/1‑218 (MQ=255)                                                                                                                                      gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTaa                                                                                                           >  1:40166/1‑239 (MQ=255)                                                                                                                                      gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGt                                                                                                  <  1:837418/248‑1 (MQ=255)                                                                                                                                      gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCt                                                                                                <  1:330104/250‑1 (MQ=255)                                                                                                                                              cTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTac                                                                                                                                                       >  1:517768/1‑187 (MQ=255)                                                                                                                                                    tACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGa                                                                                 <  1:253074/251‑1 (MQ=255)                                                                                                                                                    tACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGa                                                                                 >  1:31120/1‑251 (MQ=255)                                                                                                                                                       cAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAg                                                                                                                                                                                                     >  1:287701/1‑132 (MQ=255)                                                                                                                                                            gCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGaa                                                                          <  1:497042/250‑1 (MQ=255)                                                                                                                                                              ttttCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTg                                                                       >  1:126043/1‑251 (MQ=255)                                                                                                                                                                         ccccGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTc                                                            <  1:77677/251‑1 (MQ=255)                                                                                                                                                                             gACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  >  1:362487/1‑177 (MQ=255)                                                                                                                                                                                  caTTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGt                                                   >  1:241953/1‑251 (MQ=255)                                                                                                                                                                                  caTTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTG‑TTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGtt                                                  >  1:117127/1‑251 (MQ=255)                                                                                                                                                                                          cTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:597606/163‑1 (MQ=255)                                                                                                                                                                                          cTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTc                                                            <  1:272419/234‑1 (MQ=255)                                                                                                                                                                                               aGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         <  1:706996/104‑1 (MQ=255)                                                                                                                                                                                                    gCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACa                                 >  1:405626/1‑251 (MQ=255)                                                                                                                                                                                                     ccccATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTacac                                                                                                                                                     >  1:461732/1‑134 (MQ=255)                                                                                                                                                                                                     ccccATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACaa                                >  1:305536/1‑251 (MQ=255)                                                                                                                                                                                                        cATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAAc                             <  1:13503/251‑1 (MQ=255)                                                                                                                                                                                                         aTGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          >  1:250963/1‑61 (MQ=255)                                                                                                                                                                                                                 cAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGc                     <  1:818191/250‑1 (MQ=255)                                                                                                                                                                                                                  aaaGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGccc                   <  1:297078/251‑1 (MQ=255)                                                                                                                                                                                                                         cgcCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACg             <  1:608647/250‑1 (MQ=255)                                                                                                                                                                                                                           cccACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGaat          <  1:868274/251‑1 (MQ=255)                                                                                                                                                                                                                             cacaGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTg                                                 <  1:103465/210‑1 (MQ=255)                                                                                                                                                                                                                                 gAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTaa    >  1:677507/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAt  >  1:492491/1‑250 (MQ=255)                                                                                                                                                                                                                                              cGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAAc                             <  1:379191/213‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            GCAATAATCGGTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTGTCGCCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAT  >  1/664197‑664673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑