Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	663,621	A→G	intergenic (+53447/+29736)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	663,621	0	A	G	100.0%	199.9 / NA	69	intergenic (+53447/+29736)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/44);  total (25/44)

CGCCGTGATGGTGCCGTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCATGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGGTGTAAGTGCCGACGACGCTCACGTTTTCGGCAGAAAATCCCTGCA  >  1/663377‑663867                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cgcCGTGATGGTGCCGTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACTGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAAt                                                                                                                                                                                                                                                  <  1:451547/251‑1 (MQ=255)     gTGATGGTGCCGTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTAt                                                                                                                                                                                                                                               >  1:672197/1‑250 (MQ=255)     gTGATGGTGCCGTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTAt                                                                                                                                                                                                                                               >  1:526315/1‑250 (MQ=255)                gTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAatca                                                                                                                                                                                                                                   >  1:287088/1‑251 (MQ=255)                  tGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAatcatc                                                                                                                                                                                                                                 <  1:872171/251‑1 (MQ=255)                     gATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCAtct                                                                                                                                                                                                                                >  1:233110/1‑249 (MQ=255)                       tCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGt                                                                                                                                                                                                                            <  1:2591/251‑1 (MQ=255)                          atatTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGt                                                                                                                                                                                                                            <  1:65315/248‑1 (MQ=255)                            atTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAAc                                                                                                                                                                                                                       <  1:285845/251‑1 (MQ=255)                              tttCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAAccg                                                                                                                                                                                                                     <  1:873172/251‑1 (MQ=255)                                       ttACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAata                                                                                                                                                                                                            <  1:261868/251‑1 (MQ=255)                                        tACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAatat                                                                                                                                                                                                           >  1:508558/1‑251 (MQ=255)                                         ccTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACTTGACGGCTTTCGTAAATTAACGGCGTGGAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATc                                                                                                                                                                                                          <  1:90927/250‑1 (MQ=255)                                         aCTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAatat                                                                                                                                                                                                           >  1:305636/1‑250 (MQ=255)                                              aTGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGt                                                                                                                                                                                                                            >  1:113669/1‑228 (MQ=255)                                              aTGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATaa                                                                                                                                                                                                     <  1:871220/251‑1 (MQ=255)                                              aTGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATaa                                                                                                                                                                                                     >  1:353132/1‑251 (MQ=255)                                              aTGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATaa                                                                                                                                                                                                     >  1:49277/1‑251 (MQ=255)                                                   cGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAAt                                                                                                                                                                                                >  1:342688/1‑251 (MQ=255)                                                     gTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAAt                                                                                                                                                                                                <  1:638267/249‑1 (MQ=255)                                                          gATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGt                                                                                                                                                                                          >  1:110640/1‑250 (MQ=255)                                                           aTTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGtc                                                                                                                                                                                         <  1:616133/250‑1 (MQ=255)                                                           aTTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGtc                                                                                                                                                                                         <  1:891404/250‑1 (MQ=255)                                                             tGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGtccgg                                                                                                                                                                                      <  1:798394/251‑1 (MQ=255)                                                              gTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGtccggt                                                                                                                                                                                     >  1:762668/1‑251 (MQ=255)                                                              gTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGtccggt                                                                                                                                                                                     >  1:452405/1‑251 (MQ=255)                                                               ttCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAata                                                                                                                                                                                                            <  1:238158/227‑1 (MQ=255)                                                                         aCCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAAc                                                                                                                                                                                                                       <  1:512893/206‑1 (MQ=255)                                                                          cccAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTgggg                                                                                                                                                                         >  1:345181/1‑250 (MQ=255)                                                                                aCCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCa                                                                                                                                                                   <  1:505136/251‑1 (MQ=255)                                                                                 ccGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCATTGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCaa                                                                                                                                                                  <  1:639621/251‑1 (MQ=255)                                                                                 ccGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCaa                                                                                                                                                                  <  1:324519/251‑1 (MQ=255)                                                                                    aTTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAAc                                                                                                                                                                <  1:692217/250‑1 (MQ=255)                                                                                     ttGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACg                                                                                                                                                               >  1:398132/1‑250 (MQ=255)                                                                                                ggTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCAtct                                                                                                                                                                                                                                <  1:89856/174‑1 (MQ=255)                                                                                                  ttACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATgg                                                                                                                                                  <  1:503006/250‑1 (MQ=255)                                                                                                     cGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATaa                                                                                                                                                                                                     >  1:234348/1‑196 (MQ=255)                                                                                                     cGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGtttt                                                                                                                                              >  1:98878/1‑251 (MQ=255)                                                                                                       gTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGaaa                                                                                                                                                                                                                                                   <  1:505669/148‑1 (MQ=255)                                                                                                       gTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAAtt                                                                                                                                                                                               <  1:307159/200‑1 (MQ=255)                                                                                                       gTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTgg                                                                                                                                            >  1:415557/1‑251 (MQ=255)                                                                                                         aTTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTgg                                                                                                                                            >  1:468081/1‑249 (MQ=255)                                                                                                         aTTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCt                                                                                                                                          <  1:559540/251‑1 (MQ=255)                                                                                                          ttCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCtt                                                                                                                                         <  1:735757/251‑1 (MQ=255)                                                                                                           tCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCa                                                                                                                                                                   >  1:648484/1‑224 (MQ=255)                                                                                                                      aTCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTCCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCg                                                                                                                              <  1:429062/250‑1 (MQ=255)                                                                                                                       tCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCg                                                                                                                              <  1:840984/249‑1 (MQ=255)                                                                                                                             aTTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCCTCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTaataat                                                                                                                      >  1:321200/1‑251 (MQ=255)                                                                                                                             aTTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCtaat                                                                                                                         <  1:180273/248‑1 (MQ=255)                                                                                                                                ttATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATAtt                                                                                                                   <  1:118248/251‑1 (MQ=255)                                                                                                                                ttATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATAtt                                                                                                                   <  1:454829/251‑1 (MQ=255)                                                                                                                                    tCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAAtt                                                                                                               <  1:137353/251‑1 (MQ=255)                                                                                                                                       aTGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAAttt                                                                                                                                                                                              <  1:6862/169‑1 (MQ=255)                                                                                                                                           aCTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAAtt                                                                                                               <  1:780631/244‑1 (MQ=255)                                                                                                                                              ttGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGaa                                                                                                     >  1:624220/1‑251 (MQ=255)                                                                                                                                                gTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGttt                                                                                                                                   <  1:1745/219‑1 (MQ=255)                                                                                                                                                   ttgcttgcCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACg                                                                                                <  1:725236/251‑1 (MQ=255)                                                                                                                                                   ttgcttgcCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACg                                                                                                <  1:718479/251‑1 (MQ=255)                                                                                                                                                    tgcttgcCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGt                                                                                               <  1:347193/251‑1 (MQ=255)                                                                                                                                                                            gATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACAcgt                                                                       >  1:878766/1‑251 (MQ=255)                                                                                                                                                                                      ttCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTAc                                                             <  1:769041/251‑1 (MQ=255)                                                                                                                                                                                      ttCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTAc                                                             <  1:360825/251‑1 (MQ=255)                                                                                                                                                                                                aTTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGcgtcgt                                                   <  1:486190/251‑1 (MQ=255)                                                                                                                                                                                                    tttCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAgg                                               <  1:15895/251‑1 (MQ=255)                                                                                                                                                                                                     ttCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGgt                                              <  1:750637/251‑1 (MQ=255)                                                                                                                                                                                                             aCTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGGTGTAAGTGc                                      <  1:505040/251‑1 (MQ=255)                                                                                                                                                                                                                      gCCCACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATg                                                                                                                                                   <  1:759740/133‑1 (MQ=255)                                                                                                                                                                                                                        ccACATGACGGCTTTCGTAAATTAACGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGGTGTAAGTg                                       >  1:34842/1‑239 (MQ=255)                                                                                                                                                                                                                                                 cGGCGTGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGGTGTAAGTGCCGACGACGCTCACGTTTTCGGCAGAAAATCCCTGCa  >  1:105119/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGCCGTGATGGTGCCGTTGGGATCGATATTTTCGTAAATTACTTGATGCACGGTTTTGATTGTTCCTGATGAACCCAGCACCGATTGCCAACCCAGGTTACGGTATTCCCAGGCGAGATCTTCAATTTTATTCAATGCACTTTGTTTTGCTTGCCGGAAATTTTCCGCGCTGATTTCGCCGTTCGGAAAGAATTTTTTCGCAAAACTGACGCAGCCCACATGACGGCTTTCGTAAATTAACGGCATGAAATTATCGCCGATAATCATCTCTGTTGAACCGCCGCCAATATCAATAACGAATTTACGTCCGGTTTCCGGTTGGGTGTGGCAAACGCCCGCGTAGATGGTTTTGGCTTCGGTTTTGCCGCTAATAATATTAATTTGATAAGGAAATACGTTGGCGGCTTGGCGTAAAAACACGTCGTTATTTACCGCGCGTCGTAAGGTGTAAGTGCCGACGACGCTCACGTTTTCGGCAGAAAATCCCTGCA  >  1/663377‑663867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑