Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	658,677	A→G	intergenic (+48503/+34680)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	658,677	0	A	G	100.0%	181.5 / NA	64	intergenic (+48503/+34680)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/35);  total (29/35)

GTGTCAAACACCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACAGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTCCGCATTGCGGCACAGG  >  1/658432‑658914                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gtgtCAAACACCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGt                                                                                                                                                                                                                                           <  1:63297/250‑1 (MQ=255)   gtCAAACACCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCa                                                                                                                                                                                                                                        >  1:294096/1‑251 (MQ=255)     cAAACACCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCAtt                                                                                                                                                                                                                                      <  1:370926/251‑1 (MQ=255)         cacCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACat                                                                                                                                                                                                                                  <  1:499059/251‑1 (MQ=255)                aCCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAg                                                                                                                                                                                                                           <  1:524625/251‑1 (MQ=255)                    cTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTc                                                                                                                                                                                                                        <  1:296527/250‑1 (MQ=255)                                    aaTTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACat                                                                                                                                                                                                                                  >  1:412603/1‑224 (MQ=255)                                         gtgCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACat                                                                                                                                                                                                                                  >  1:573659/1‑219 (MQ=255)                                                cAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAAtttt                                                                                                                                                                                           >  1:201954/1‑251 (MQ=255)                                                      cAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTc                                                                                                                                                                                                                  >  1:159627/1‑222 (MQ=255)                                                                        gAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATaa                                                                                                                                                                   >  1:812878/1‑251 (MQ=255)                                                                           tttATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCTCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 <  1:429938/250‑1 (MQ=255)                                                                           tttATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 <  1:680463/250‑1 (MQ=255)                                                                           tttATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 <  1:799863/250‑1 (MQ=255)                                                                              aTCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 <  1:881514/247‑1 (MQ=255)                                                                                           tGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGc                                                                                                                                                                                                                             <  1:331611/174‑1 (MQ=255)                                                                                             cAATAAGTGCGGTTCGGCATACAATATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGaa                                                                                                                                                                                                       <  1:432235/194‑1 (MQ=255)                                                                                                   gTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTccc                                                                                                                                           >  1:70910/1‑248 (MQ=255)                                                                                                        gTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGc                                                                                                                                       <  1:24624/247‑1 (MQ=255)                                                                                                        gTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCa                                                                                                                                   >  1:729830/1‑251 (MQ=255)                                                                                                          tCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGc                                                                                                                                                                            <  1:825204/208‑1 (MQ=255)                                                                                                           cGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAg                                                                                                                                <  1:24306/251‑1 (MQ=255)                                                                                                                         ttttttATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGccc                                                                                                                  <  1:731368/251‑1 (MQ=255)                                                                                                                              tATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATgg                                                                                                             <  1:325059/251‑1 (MQ=255)                                                                                                                              tATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATgg                                                                                                             <  1:315525/251‑1 (MQ=255)                                                                                                                                tttttGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGc                                                                                                                                                                            >  1:552554/1‑186 (MQ=255)                                                                                                                                          ggCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCg                                                                                                 >  1:440645/1‑251 (MQ=255)                                                                                                                                            cTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 >  1:133795/1‑185 (MQ=255)                                                                                                                                                         gCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGaaa                                                                                  >  1:132325/1‑251 (MQ=255)                                                                                                                                                              ccaccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCGCCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGc                                                                                                                                                                                                                             <  1:101216/107‑1 (MQ=255)                                                                                                                                                                accaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAat                                                                                          <  1:604556/236‑1 (MQ=255)                                                                                                                                                                accaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGaaaataaaat                                                                           >  1:889363/1‑251 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGTGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATgg                                                                                                             >  1:327399/1‑216 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACat                                                                                                                                                                                                                                  <  1:587270/99‑1 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGAt                                                                                                                                                                     >  1:184229/1‑160 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCt                                                                                                                              <  1:720499/199‑1 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATgg                                                                                                             >  1:88833/1‑216 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATgg                                                                                                             <  1:13868/216‑1 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCgg                                                                                     >  1:49683/1‑240 (MQ=255)                                                                                                                                                                 ccaTATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGaaaataaaat                                                                           >  1:886990/1‑250 (MQ=255)                                                                                                                                                                   atatAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACat                                                                                                                                                                                                                                  <  1:838180/97‑1 (MQ=255)                                                                                                                                                                   atatAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATcg                                                                         >  1:895294/1‑250 (MQ=255)                                                                                                                                                                        ggTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGaa                                                                                                                                                                                                       <  1:571514/119‑1 (MQ=255)                                                                                                                                                                                  gTCCATGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCAc                                                                                                                                     <  1:451507/175‑1 (MQ=255)                                                                                                                                                                                  gTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATa                                                         >  1:72668/1‑251 (MQ=255)                                                                                                                                                                                   tCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAg                                                        <  1:168561/251‑1 (MQ=255)                                                                                                                                                                                                     aaCCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTaa                                       >  1:822347/1‑250 (MQ=255)                                                                                                                                                                                                     aaCCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAAc                                      <  1:421059/251‑1 (MQ=255)                                                                                                                                                                                                      aCCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGc                                            <  1:736255/244‑1 (MQ=255)                                                                                                                                                                                                              gTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCAcc                                                                                                                                    >  1:340717/1‑148 (MQ=255)                                                                                                                                                                                                              gTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTca                             >  1:855203/1‑251 (MQ=255)                                                                                                                                                                                                              gTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTca                             >  1:632943/1‑251 (MQ=255)                                                                                                                                                                                                                    cctcacctTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGcc                       <  1:498363/251‑1 (MQ=255)                                                                                                                                                                                                                    cctcacctTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGcc                       <  1:534858/251‑1 (MQ=255)                                                                                                                                                                                                                         cctTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTGCATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTc                  <  1:465776/251‑1 (MQ=255)                                                                                                                                                                                                                         cctTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 <  1:552268/108‑1 (MQ=255)                                                                                                                                                                                                                         cctTTCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTc                  <  1:32948/251‑1 (MQ=255)                                                                                                                                                                                                                           tttCAGTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTCCg                >  1:360293/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTCACGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAAcc                                                                                                                                                                 >  1:286561/1‑101 (MQ=255)                                                                                                                                                                                                                                     cGGCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCt                                                                                                                              >  1:294445/1‑131 (MQ=255)                                                                                                                                                                                                                                       gCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGc                                                                                                                                       >  1:769598/1‑120 (MQ=255)                                                                                                                                                                                                                                       gCGAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGaaaataaaat                                                                           <  1:589790/180‑1 (MQ=255)                                                                                                                                                                                                                                         gAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATcgc                                                                        <  1:356920/181‑1 (MQ=255)                                                                                                                                                                                                                                         gAATAGTGCGCACGGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTCCGCATTGCGGCACAgg  >  1:270204/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GTGTCAAACACCATCACCGCTTGCGCACCCGCTTTAATTTGTGCATTCAAATACAAAATAACGCTGTCGGCGAGTTTATCCAACAACGCGTGCAATAAGTGCGGTTCGGCATACAACATTTTTTTTATTTTTGTGAAGGCTTTGGAAGAACTGCCCTCCACCATATAGGTCGCCAAGGTCCACGGGCTGCCGGAGAAACCGATGAGTGGCACCTCACCTTTCAGTTCACGGCGAATAGTGCGCACAGCGTTCATTACATATTGCAGCTCCTGTTCCGGGTCAGGAACGGGTAAATTTTCCACCGCACTTTTGCTGTCGATAACCCGTTCAAATTTCGGTCCTTCCCCTGCACCAAAGCTTAATCCCAGCCCCATGGCGTCAGGAATCGTTAAAATATCGGAAAATAAAATCGCCGCGTCCAAATCATAGCGACGCAATGGCTGTAACGTCACCTCACACGCCAATTCCGCATTGCGGCACAGG  >  1/658432‑658914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑