Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	653,757	A→G	intergenic (+43583/+39600)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	653,757	0	A	G	100.0%	191.7 / NA	67	intergenic (+43583/+39600)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/35);  total (32/35)

GAAGCCAAGTTTCTACTATTCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGACGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCATCTCCCTTCT  >  1/653514‑653998                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gAAGCCAAGTTTCTACTATTCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCAc                                                                                                                                                                                                                                             <  1:229403/250‑1 (MQ=255)        aGTTTCTACTATTCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCAcg                                                                                                                                                                                                                                     >  1:352708/1‑251 (MQ=255)                ctaTTCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACt                                                                                                                                                                                                                              >  1:369952/1‑250 (MQ=255)                   ttCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACtcat                                                                                                                                                                                                                           >  1:670714/1‑250 (MQ=255)                         aaCGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAacac                                                                                                                                                                                                                     <  1:517675/250‑1 (MQ=255)                                  cacaAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGACCTCATCAACACGCCATTATTg                                                                                                                                                                                                           <  1:703756/251‑1 (MQ=255)                                  cacaAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTg                                                                                                                                                                                                           <  1:768098/251‑1 (MQ=255)                                  cacaAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTg                                                                                                                                                                                                           <  1:192620/251‑1 (MQ=255)                                  cacaAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTg                                                                                                                                                                                                           <  1:519124/251‑1 (MQ=255)                                    caAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCGCCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGAt                                                                                                                                                                                                         <  1:623867/251‑1 (MQ=255)                                    caAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGAt                                                                                                                                                                                                         <  1:113276/251‑1 (MQ=255)                                    caAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGAt                                                                                                                                                                                                         <  1:294118/251‑1 (MQ=255)                                      aGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCg                                                                                                                                                                                                       >  1:797262/1‑251 (MQ=255)                                      aGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCg                                                                                                                                                                                                       >  1:888605/1‑251 (MQ=255)                                                     tACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAg                                                                                                                                                                                        >  1:438551/1‑251 (MQ=255)                                                     tACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAg                                                                                                                                                                                        >  1:407072/1‑251 (MQ=255)                                                      aCCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGc                                                                                                                                                                                       <  1:892066/251‑1 (MQ=255)                                                             aagCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACAcc                                                                                                                                                                                <  1:22733/251‑1 (MQ=255)                                                               gCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACAcc                                                                                                                                                                                >  1:676581/1‑249 (MQ=255)                                                                      gCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         <  1:394363/249‑1 (MQ=255)                                                                      gCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         <  1:867519/249‑1 (MQ=255)                                                                      gCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTatat                                                                                                                                                                        <  1:885784/250‑1 (MQ=255)                                                                          cGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         <  1:750568/245‑1 (MQ=255)                                                                             ggAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAac                                                                                                                                                                                                                       <  1:815636/196‑1 (MQ=255)                                                                              gAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACaa                                                                                                                                                               >  1:541630/1‑251 (MQ=255)                                                                                                    tGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCAACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCg                                                                                                                                         >  1:330492/1‑251 (MQ=255)                                                                                                              cACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAg                                                                                                                               <  1:539267/251‑1 (MQ=255)                                                                                                                    gtgtaaCGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         >  1:630406/6‑203 (MQ=255)                                                                                                                                    aTGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAAt                                                                                                                            <  1:560261/232‑1 (MQ=255)                                                                                                                                    aTGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGgag                                                                                                          >  1:681271/1‑250 (MQ=255)                                                                                                                                             ggCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGTCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGACCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCatat                                                                                                >  1:242371/1‑251 (MQ=255)                                                                                                                                             ggCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAAt                                                                                                                            >  1:434265/1‑223 (MQ=255)                                                                                                                                             ggCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCatat                                                                                                >  1:141338/1‑251 (MQ=255)                                                                                                                                             ggCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCatat                                                                                                >  1:526213/1‑251 (MQ=255)                                                                                                                                             ggCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTACGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCatat                                                                                                >  1:710728/1‑251 (MQ=255)                                                                                                                                                  ataCAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         <  1:1102/173‑1 (MQ=255)                                                                                                                                                             gTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTg                                                                                                                                                                                                           <  1:352209/128‑1 (MQ=255)                                                                                                                                                             gTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGgag                                                                                                          >  1:715124/1‑225 (MQ=255)                                                                                                                                                             gTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAg                                                                                >  1:512352/1‑251 (MQ=255)                                                                                                                                                                    aTACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAat                                                                         <  1:586452/251‑1 (MQ=255)                                                                                                                                                                    aTACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAat                                                                         <  1:399175/251‑1 (MQ=255)                                                                                                                                                                       ctacGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTtata                                                                                                                                                                         <  1:159019/152‑1 (MQ=255)                                                                                                                                                                       ctacGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTatat                                                                                                                                                                        <  1:876224/153‑1 (MQ=255)                                                                                                                                                                       ctacGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGaa                                                                                                                                                     <  1:560960/172‑1 (MQ=255)                                                                                                                                                                         acGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATAtgtg                                                                    <  1:273539/251‑1 (MQ=255)                                                                                                                                                                            gCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATc                                                                                               >  1:73667/1‑221 (MQ=255)                                                                                                                                                                             cATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAAc                                                                <  1:503912/251‑1 (MQ=255)                                                                                                                                                                                cacccaccGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACaaa                                                             >  1:557056/1‑251 (MQ=255)                                                                                                                                                                                 acccaccGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAAt                                                            >  1:860510/1‑251 (MQ=255)                                                                                                                                                                                  cccaccGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAAt                                                            >  1:568787/1‑250 (MQ=255)                                                                                                                                                                                         ccACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATg                                                                                                                                                                       <  1:50889/136‑1 (MQ=255)                                                                                                                                                                                                       cGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGAttt                                       >  1:574873/1‑250 (MQ=255)                                                                                                                                                                                                         ccACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAAGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCtt                                    >  1:102127/1‑251 (MQ=255)                                                                                                                                                                                                           aCGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAAc                                                                <  1:243770/221‑1 (MQ=255)                                                                                                                                                                                                           aCGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTg                                  <  1:194117/251‑1 (MQ=255)                                                                                                                                                                                                                 cacCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAAcc                                                                                         <  1:759617/190‑1 (MQ=255)                                                                                                                                                                                                                 cacCATGGGTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGgaaga                            >  1:375371/1‑251 (MQ=255)                                                                                                                                                                                                                       gggTAGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGaa                                                                                                                                                     <  1:564397/124‑1 (MQ=255)                                                                                                                                                                                                                           aGCTTGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAata                                                                        >  1:495445/1‑197 (MQ=255)                                                                                                                                                                                                                              ttGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAacac                                                                                                                                                                                                                     <  1:772681/53‑1 (MQ=255)                                                                                                                                                                                                                              ttGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCaa                                                                                                                                      <  1:249813/132‑1 (MQ=255)                                                                                                                                                                                                                               tGTTAGACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCttt              >  1:839327/1‑251 (MQ=255)                                                                                                                                                                                                                                    gACTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATAtg                                                                      >  1:440441/1‑190 (MQ=255)                                                                                                                                                                                                                                     aCTTAATGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCAtc          <  1:728602/249‑1 (MQ=255)                                                                                                                                                                                                                                         aaTGACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCATCTCCCtt    >  1:14261/1‑251 (MQ=255)                                                                                                                                                                                                                                            gACATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCATCTCCCTtct  >  1:109745/1‑250 (MQ=255)                                                                                                                                                                                                                                             aCATGGGGCGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCATCTcc       >  1:318882/1‑244 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GAAGCCAAGTTTCTACTATTCAAGAACGCCTTGCACAAGCGGCGGCAGCGCGTACCGAAGAAGCCAAAGGCAACGTGGAATACGCAGCGAATGCGGATATGAGCAAAATCACCGAAGCGGACGTGGCGAAAATGCCTGCTGGCTTATACAAAGACGGTTTTGAATACTACGGCATCACCCACCGCCACCCAAACAGCACGCCACGTTACACCATGGGTAGCTTGTTAGACTTAATGACATGGGACGCCACCGACCACGCGGAACTCATCAACACGCCATTATTGATCGTGGCAGGCGACAAAGCCGACACCCTTTATATGAGCGACAAAGCCTTTGAAAAAGCAGGTTCGCAAGACAAAGAATTGGTGAAAATCCCAGGAGCGAGCCATATCGAAACCTACTGGAAGCCTGAATATGTGAAACAAATCAGCGAGAAATTGACGGATTTCTTTGGGAAGAAATTGTAATTCTTTCATCTCCCTTCT  >  1/653514‑653998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑