Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	646,441	C→T	intergenic (+36267/+46916)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	646,441	0	C	T	100.0%	122.9 / NA	43	intergenic (+36267/+46916)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

CGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTCACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGGCAATTTTCGTCAGAGAGAGGGGGAACGATATTGATTTTCCCCTCTCCCTCCGAG  >  1/646197‑646622                                                                                                                                                                                                                                                     |                                                                                                                                                                                      cGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTTTTGAATATTATTTACAAgg                                                                                                                                                                                 >  1:763640/1‑251 (MQ=255) cGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAgg                                                                                                                                                                                 <  1:141136/251‑1 (MQ=255)   gCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                <  1:96730/250‑1 (MQ=255)   gCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                <  1:142818/250‑1 (MQ=255)   gCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                <  1:744328/250‑1 (MQ=255)   gCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                <  1:594633/250‑1 (MQ=255)              tGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGAttttt                                                                                                                                                                    >  1:897853/1‑251 (MQ=255)                aaaCAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTc                                                                                                                                                                  <  1:215338/251‑1 (MQ=255)                                        ggtggCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGtt                                                                                                                                          >  1:138549/1‑251 (MQ=255)                                        ggtagCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATAGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGGGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGtt                                                                                                                                          >  1:138537/5‑251 (MQ=255)                                           ggCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGttttt                                                                                                                                       >  1:308798/1‑251 (MQ=255)                                              aaTGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTtat                                                                                                                                    <  1:720044/251‑1 (MQ=255)                                              aaTGCTGCGTGTGGTGCGGTTGAACAGTTGGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAGACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGATGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTtat                                                                                                                                    <  1:592760/251‑1 (MQ=255)                                                tgctgcGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATaa                                                                                                                                  <  1:331281/251‑1 (MQ=255)                                                           gtgCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGTATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATTAAAGTTTTTTATAATGCACAAATCa                                                                                                                       >  1:591242/1‑251 (MQ=255)                                                           gtgCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTtat                                                                                                                                    <  1:437147/238‑1 (MQ=255)                                                             gCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGGTCTATTGTTGAATATTTTTTACAAAGCTATTCGATTTTTTTCTACTAATCAAATTAATCAAAATTTTTTTTAATGCACAAATCaaa                                                                                                                     >  1:251992/1‑251 (MQ=255)                                                                gTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAaaac                                                                                                                   <  1:587850/250‑1 (MQ=255)                                                                gTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGGGAAACTGCCTTCTTGCTCCGCGGTGATAAAGGTGCGAAGTTCGTTGTTGTTGCCGATCATAGGCGGGGTTTTTTTGTTGGATATTTTTTTCAAGGGTATCCGTTTTTTTTCTACCAATCAAATGAATCAAAGGTTTTTTTAATGCACAAATCAaaacg                                                                                                                  >  1:171899/1‑251 (MQ=255)                                                                        gTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGGGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCgg                                                                                                          >  1:138581/1‑251 (MQ=255)                                                                             aTGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGaataa                                                                                                     >  1:64928/1‑251 (MQ=255)                                                                             aTGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGGGGTCTATTGTTGAATATTATTTGCAAGGCTATTCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAAACAAAACGAAACGCGGaataa                                                                                                     >  1:449476/1‑251 (MQ=255)                                                                                ttGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATcc                                                                                                  <  1:829773/251‑1 (MQ=255)                                                                                       gTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTTCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGc                                                                                            >  1:599642/1‑250 (MQ=255)                                                                                       gTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATTCACAAATCAAAACGAAACGCGGAATAATCCTCTCGc                                                                                            >  1:16529/1‑250 (MQ=255)                                                                                                               ggCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCg                                                                                             <  1:808707/225‑1 (MQ=255)                                                                                                                 cTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAAt                                                                 <  1:166508/251‑1 (MQ=255)                                                                                                                 cTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAAt                                                                 <  1:716934/251‑1 (MQ=255)                                                                                                                                     gTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                >  1:561380/1‑120 (MQ=255)                                                                                                                                                      cTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGa                            <  1:536780/251‑1 (MQ=255)                                                                                                                                                      cTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGa                            <  1:525282/251‑1 (MQ=255)                                                                                                                                                        ttGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGata                          <  1:175768/251‑1 (MQ=255)                                                                                                                                                        ttGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGata                          <  1:744488/251‑1 (MQ=255)                                                                                                                                                                aCTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGATTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcgtta                   >  1:417501/1‑243 (MQ=255)                                                                                                                                                                    ccTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcgttacctc               <  1:609351/250‑12 (MQ=255)                                                                                                                                                                                 cgGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcgttacctccacgtcgttttat  <  1:841101/250‑25 (MQ=255)                                                                                                                                                                                   gTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcg                      <  1:183131/228‑5 (MQ=255)                                                                                                                                                                                                     tCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGaat                                                                                                       >  1:584309/1‑129 (MQ=255)                                                                                                                                                                                                                gtCGATCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGaat                                                                                                       <  1:647723/118‑1 (MQ=255)                                                                                                                                                                                                                    aTCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGc                                                                                                                                                                                >  1:514684/1‑41 (MQ=255)                                                                                                                                                                                                                    aTCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcgttacctccacgt          >  1:281062/1‑191 (MQ=255)                                                                                                                                                                                                                    aTCATAGGCGGTGTTCTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcg                      >  1:250396/1‑191 (MQ=255)                                                                                                                                                                                                                                   cTATTGTTGAATATTATTTACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGACAATTTTCGTCAGAGAGAGGGGGAACGatacgcgttacctc               >  1:780006/1‑176 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                      CGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTGCGCCGCGGTGATAAAGGTGCGAAGTTCGTTGATGTTGTCGATCATAGGCGGTGTTCTATTGTTGAATATTATTCACAAGGCTATCCGATTTTTTCCTACTAATCAAATGAATCAAAGTTTTTTATAATGCACAAATCAAAACGAAACGCGGAATAATCCTCTCGCCCGCTTGAGGGAGAGAGAGACGGAAATTGCGTCAGGCAATTTTCGTCAGAGAGAGGGGGAACGATATTGATTTTCCCCTCTCCCTCCGAG  >  1/646197‑646622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑