Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	646,127	A→G	intergenic (+35953/+47230)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	646,127	0	A	G	100.0%	174.5 / NA	61	intergenic (+35953/+47230)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/23);  total (38/23)

ACTCTGGCGTGCCGACCACCGCCATTTGCATTTTGTCGCTTAATCTCACGGCGATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCATAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTG  >  1/645896‑646367                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aCTCTGGCGTGCCGACCACCGCCATTTGCATTTTGTCGCTTAATCTCACGGCGATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACgtg                                                                                                                                                                                                                               >  1:423877/1‑251 (MQ=255)                   ccGCCATTTGCATTTTGTCGCTTAATCTCACGGCGATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAAt                                                                                                                                                                                                             >  1:61338/1‑251 (MQ=255)                                           aTCTCACGGCGATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACGTGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAgg                                                                                                                                                                                                                     >  1:450468/1‑219 (MQ=255)                                           aTCTCACGGCGATCATATCTTGTCCCCCGTCCGATCCTAAGCGAATCCCCGCATCAACCCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATAc                                                                                                                                                                                       <  1:699303/249‑1 (MQ=255)                                                 cggcgataattttttggcccccgcccGATCCTAAGCAAATACCCCAATCAACCCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAaac                                                                                                                                                                               <  1:255541/227‑1 (MQ=255)                                                 cgccgttcttatCTTGTCCCCCGTCCGTTCTTAAGCGAATCCCCCCACCAACCCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAaac                                                                                                                                                                               <  1:32918/242‑1 (MQ=255)                                                     gATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACg                                                                                                                                                                           >  1:318012/1‑251 (MQ=255)                                                      aTCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTcc                                                                                                                                                                                                                                          >  1:494427/1‑187 (MQ=255)                                                      aTCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACgg                                                                                                                                                                          >  1:862727/1‑251 (MQ=255)                                                      aTCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACgg                                                                                                                                                                          >  1:332983/1‑251 (MQ=255)                                                        cATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATg                                                                                                                                                                                                            >  1:524955/1‑215 (MQ=255)                                                          tatCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAag                                                                                                                                                                      >  1:530289/1‑251 (MQ=255)                                                             cTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAaaca                                                                                                                                                                              >  1:194527/1‑240 (MQ=255)                                                                gtgCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGAttt                                                                                                                                                                >  1:739826/1‑251 (MQ=255)                                                                            aTCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATc                                                                                                                                                                                               >  1:48893/1‑208 (MQ=255)                                                                            aTCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTg                                                                                                                                                     >  1:523823/1‑250 (MQ=255)                                                                             tCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTg                                                                                                                                                     >  1:382284/1‑249 (MQ=255)                                                                                 aaGCGAATCCCCGCATCAACCCCCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTcgcc                                                                                                                                               <  1:473053/251‑1 (MQ=255)                                                                                   gCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTcgcccg                                                                                                                                             >  1:316854/1‑251 (MQ=255)                                                                                     aaatccccGCATCAACCCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTcgcccgcc                                                                                                                                           <  1:587449/246‑1 (MQ=255)                                                                                              cATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAgaga                                                                                                                                                                   >  1:540422/1‑218 (MQ=255)                                                                                              cATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCgtggtg                                                                                                                                  >  1:781394/1‑251 (MQ=255)                                                                                                ccAAACCCCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGc                                                                                                                                <  1:335889/250‑1 (MQ=255)                                                                                                     cccccccgccACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAAtgc                                                                                                                           <  1:160310/248‑1 (MQ=255)                                                                                                           cgccACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCgtg                                                                                                                     <  1:42421/251‑1 (MQ=255)                                                                                                             ccACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCgtgt                                                                                                                    <  1:115993/250‑1 (MQ=255)                                                                                                                cGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTgtggtg                                                                                                                <  1:1920/251‑1 (MQ=255)                                                                                                                cGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTgtggtg                                                                                                                >  1:238004/1‑251 (MQ=255)                                                                                                                      ccACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTg                                                                                                          <  1:458577/251‑1 (MQ=255)                                                                                                                       cacaAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGa                                                                                                         >  1:387640/1‑251 (MQ=255)                                                                                                                         caAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGaa                                                                                                        >  1:132477/1‑250 (MQ=255)                                                                                                                           aaTTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAg                                                                                                     <  1:233893/251‑1 (MQ=255)                                                                                                                           aaTTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAg                                                                                                     <  1:247164/251‑1 (MQ=255)                                                                                                                                    tCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGt                                                                                             <  1:140811/250‑1 (MQ=255)                                                                                                                                         ggTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTGCCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAg                                                                                         >  1:278873/1‑249 (MQ=255)                                                                                                                                         ggTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAg                                                                                         >  1:567557/1‑249 (MQ=255)                                                                                                                                              attccaattccaTTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATg                                                                                                                                                                                                            <  1:577746/129‑1 (MQ=255)                                                                                                                                              attccaattccaTTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAg                                                                                                     >  1:882302/1‑232 (MQ=255)                                                                                                                                                 ccaattccaTTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAAt                                                                                                                                                                                                             <  1:557262/125‑1 (MQ=255)                                                                                                                                                        caTTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTg                                                                         >  1:822879/1‑250 (MQ=255)                                                                                                                                                            tCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGc                                                                                                                                <  1:207229/191‑1 (MQ=255)                                                                                                                                                               gggTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGt                                                                                             <  1:101482/223‑1 (MQ=255)                                                                                                                                                                 gTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGc                                                                                        <  1:274588/226‑1 (MQ=255)                                                                                                                                                                 gTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCgaatggaat                                                               >  1:883937/1‑251 (MQ=255)                                                                                                                                                                 gTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCgaatggaat                                                               >  1:184338/1‑251 (MQ=255)                                                                                                                                                                      gTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCtt                                                          >  1:267683/1‑251 (MQ=255)                                                                                                                                                                          cgcgAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATg                                                      <  1:578835/251‑1 (MQ=255)                                                                                                                                                                            cgAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTACAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGc                                                     <  1:306395/250‑1 (MQ=255)                                                                                                                                                                                cGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAagag                                                                                                                                                                    <  1:611109/135‑1 (MQ=255)                                                                                                                                                                                cGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAgg                                                >  1:263335/1‑251 (MQ=255)                                                                                                                                                                                  ccATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCt                                                                                                                                                                                              <  1:101120/107‑1 (MQ=255)                                                                                                                                                                                      cAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGaaa                                          >  1:669095/1‑251 (MQ=255)                                                                                                                                                                                         tttACCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGt                                       >  1:303397/1‑251 (MQ=255)                                                                                                                                                                                           tATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCtt                                                          >  1:309761/1‑230 (MQ=255)                                                                                                                                                                                                         gATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGcggcgg                        >  1:713630/1‑250 (MQ=255)                                                                                                                                                                                                         gATAAAAAAACATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGcggcgg                        >  1:337095/1‑250 (MQ=255)                                                                                                                                                                                                                  aCATGATCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAg                                                                                                     >  1:148849/1‑164 (MQ=255)                                                                                                                                                                                                                        tCGTTACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGc         >  1:862037/1‑250 (MQ=255)                                                                                                                                                                                                                           ttACCGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCctt      >  1:882670/1‑250 (MQ=255)                                                                                                                                                                                                                              ccGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAAtgctgc                                                                                                                        >  1:549248/1‑133 (MQ=255)                                                                                                                                                                                                                              ccGTTAATGCGTAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTg  >  1:59085/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ACTCTGGCGTGCCGACCACCGCCATTTGCATTTTGTCGCTTAATCTCACGGCGATCATATCTTGTGCCACGTCCGATCCTAAGCGAATACCCGCATCAAACCGCCCCGCCACGATATCCACAAATTTGGTGTCACTGGTCAATTCCAATTCCATTTCGGGGTATTGTTCCGCGAACGCCATCAATTTATCCCACAAAATGGATAAAAAAACATGATCGTTACCGTTAATGCATAATTTCCCTTTCAACGTGTTGCGAAAGGTGCTTAATGCGTTGAGATTATCTTCAATACTTTCAAACAACGGCAAGAGATTTTGAAACAACTGCTCGCCCGCCTCCGTGGTGGCAATGCTGCGTGTGGTGCGGTTGAACAGTTTGATGTTGAGCTGTTCTTCCAGCTTGCGAATGGAATGGCTTAATGCGGAGGTGGAAACGTTTAATTTTGCGGCGGCTTTGGTGAAACTGCCTTCTTG  >  1/645896‑646367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑