Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	643,355	T→G	intergenic (+33181/+50002)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	643,355	0	T	G	100.0%	209.5 / NA	72	intergenic (+33181/+50002)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/41);  total (31/41)

CGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACTCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACTTG  >  1/643138‑643595                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 cGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAgg                                                                                                                                                                                                                                    >  1:611548/1‑232 (MQ=255)  gttgttGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGaa                                                                                                                                                                                                                                      >  1:525576/1‑229 (MQ=255)   ttgttgCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGa                                                                                                                                                                                                               >  1:600822/1‑251 (MQ=255)                     ttGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGa                                                                                                                                                                                             <  1:339281/251‑1 (MQ=255)                         aTTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                                                         <  1:819376/251‑1 (MQ=255)                         aTTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                                                         <  1:622825/251‑1 (MQ=255)                           ttGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGa                                                                                                                                                                                          >  1:831955/1‑248 (MQ=255)                                             ggCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACgg                                                                                                                                                                     >  1:201059/1‑251 (MQ=255)                                             ggCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACgg                                                                                                                                                                     >  1:676521/1‑251 (MQ=255)                                              gCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACg                                                                                                                                                                      <  1:301014/249‑1 (MQ=255)                                                     cAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCgtggtg                                                                                                                                                             <  1:851620/251‑1 (MQ=255)                                                       aTCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGgtgt                                                                                                                                                            >  1:634717/1‑250 (MQ=255)                                                         cTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGc                                                                                                                                                                                  <  1:44984/226‑1 (MQ=255)                                                            gtgtTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGAc                                                                                                                                                                                         <  1:329405/216‑1 (MQ=255)                                                             tgtTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                                                                     <  1:453958/251‑1 (MQ=255)                                                                   gcgTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCaaa                                                                                                                                                                               <  1:517186/219‑1 (MQ=255)                                                                                    ggCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                                                                     >  1:633445/1‑228 (MQ=255)                                                                                    ggCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGAt                                                                                                                              >  1:644116/1‑251 (MQ=255)                                                                                      cTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATc                                                                                                                             <  1:426015/250‑1 (MQ=255)                                                                                        tATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAAt                                                                                                                          <  1:481522/251‑1 (MQ=255)                                                                                                    aCTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTAAAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATg                                                                                                              >  1:439852/1‑251 (MQ=255)                                                                                                       tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTc                                                                                                                                                                                                                    <  1:748558/146‑1 (MQ=255)                                                                                                       tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGa                                                                                                                                                                                             >  1:890936/1‑169 (MQ=255)                                                                                                       tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                                                           <  1:104539/251‑1 (MQ=255)                                                                                                       tAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                                                           <  1:278712/251‑1 (MQ=255)                                                                                                         aaaGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                                                         <  1:242024/251‑1 (MQ=255)                                                                                                         aaaGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                                                         <  1:698798/251‑1 (MQ=255)                                                                                                             aTCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGcc                                                                                                     <  1:251472/251‑1 (MQ=255)                                                                                                                      ccATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCtt                                                                                            <  1:162629/251‑1 (MQ=255)                                                                                                                        aTGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                                                         >  1:372938/1‑236 (MQ=255)                                                                                                                         tGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTgg                                                                                         <  1:450890/251‑1 (MQ=255)                                                                                                                          gTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAgcgc                                                                                                      >  1:279619/1‑237 (MQ=255)                                                                                                                          gTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGt                                                                                        >  1:533820/1‑251 (MQ=255)                                                                                                                           ttGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                                                                       <  1:406479/251‑1 (MQ=255)                                                                                                                             gAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                                                           <  1:83342/229‑1 (MQ=255)                                                                                                                             gAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAg                                                                                                         <  1:196878/231‑1 (MQ=255)                                                                                                                                  atGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCg                                                                                >  1:659705/1‑251 (MQ=255)                                                                                                                                            aaaTGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTg                                                                      <  1:593655/251‑1 (MQ=255)                                                                                                                                               tGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTACTGGCGGAATTATTTGAg                                                                    >  1:113645/1‑250 (MQ=255)                                                                                                                                               tGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGc                                                                   <  1:58579/251‑1 (MQ=255)                                                                                                                                                         ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                                                         <  1:523140/251‑1 (MQ=255)                                                                                                                                                         ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                                                         <  1:192657/251‑1 (MQ=255)                                                                                                                                                         ttCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGa                                                         <  1:723639/251‑1 (MQ=255)                                                                                                                                                           ccAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAg                                                       <  1:262915/251‑1 (MQ=255)                                                                                                                                                               gTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAATGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                                                                       <  1:451884/215‑1 (MQ=255)                                                                                                                                                                  gAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCgtggtg                                                                                                                                                             >  1:452094/1‑142 (MQ=255)                                                                                                                                                                  gAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGa                                                >  1:741800/1‑251 (MQ=255)                                                                                                                                                                      aCGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGAccc                                             >  1:800152/1‑250 (MQ=255)                                                                                                                                                                       cGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAAt                                                                                                                                                     <  1:96056/145‑1 (MQ=255)                                                                                                                                                                         gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTAc                                                                                                           <  1:882539/185‑1 (MQ=255)                                                                                                                                                                         gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGa                                          >  1:343641/1‑250 (MQ=255)                                                                                                                                                                         gTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGa                                          >  1:147833/1‑250 (MQ=255)                                                                                                                                                                                aTCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGaaa                                                                                                                                     <  1:394359/152‑1 (MQ=255)                                                                                                                                                                                           gCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATg                       >  1:875994/1‑251 (MQ=255)                                                                                                                                                                                           gCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATg                       >  1:857765/1‑251 (MQ=255)                                                                                                                                                                                           gCGTGGGAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGGCATg                       >  1:246291/1‑251 (MQ=255)                                                                                                                                                                                                 gAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                                                                       >  1:226903/1‑181 (MQ=255)                                                                                                                                                                                                 gAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCGATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCttt                 >  1:255292/1‑251 (MQ=255)                                                                                                                                                                                                 gAGCCATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCttt                 >  1:355452/1‑251 (MQ=255)                                                                                                                                                                                                    ccATCCAACGGGCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGAc              <  1:91320/251‑1 (MQ=255)                                                                                                                                                                                                              ggCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACa                                        <  1:363901/215‑1 (MQ=255)                                                                                                                                                                                                               gCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGc                                                                                              <  1:640408/160‑1 (MQ=255)                                                                                                                                                                                                               gCATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTAATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACt    <  1:141116/250‑1 (MQ=255)                                                                                                                                                                                                                cATTATTAACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACtt   <  1:364397/250‑1 (MQ=255)                                                                                                                                                                                                                  ttattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGc                                                                   <  1:80081/184‑1 (MQ=255)                                                                                                                                                                                                                  ttattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGATGAAGCTCACTTg  >  1:828898/1‑249 (MQ=255)                                                                                                                                                                                                                  ttattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACTTg  >  1:839221/1‑249 (MQ=255)                                                                                                                                                                                                                  ttattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACTTg  >  1:698160/1‑249 (MQ=255)                                                                                                                                                                                                                   tattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTg                                                                                                                                <  1:436130/122‑1 (MQ=255)                                                                                                                                                                                                                   tattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGtt                                                                                       <  1:190995/163‑1 (MQ=255)                                                                                                                                                                                                                   tattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTc                    <  1:602648/230‑1 (MQ=255)                                                                                                                                                                                                                   tattaACGCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACTTg  >  1:179413/1‑248 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 CGTTGTTGCTCTCCCGTTTATTGAATTTGAACGCCACCCAAGAAGGCTTGCTCAATCTGGTGTTCCGCGTAGCCGACGACAAAGGCTTATTGCTTATCGACTTAAAAGATCTGCGCGCCATGTTGAAATATGTGGCGGAAAATGCCAAAACCTTCCAAGTGGAATACGGTAATGTATCCGCTGCCAGCGTGGGAGCCATCCAACGGGCATTATTAACTCTGGAAAATGAAGGCGCGGCAAATTTATTCGGCGAACCGGCGCTGAATCTGGAAGACTGGTTGCAAACCCGTGACGGTCGTGGTGTCATCAATGTGTTAAATTCGGAAAAACTGATCAATTCTCCGCGCATGTACAGCGCCTTCCTGCTTTGGTTAATGGCGGAATTATTTGAGCAATTGCCGGAAGTGGGCGACCCCGACAAACCGAAATTCGTCATGTTCTTTGACGAAGCTCACTTG  >  1/643138‑643595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑