Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	605,885	C→T	intergenic (+17570/‑4134)	unknown → / → unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	605,885	0	C	T	100.0%	101.9 / NA	35	intergenic (+17570/‑4134)	unknown/unknown	tRNA‑Arg‑TCT/tRNA‑Phe‑GAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

CGACAGCTTTTGCTCCTTCTTTATGCTGACCGCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGCCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAGGCAAACCAAGCCTTCCC  >  1/605644‑606128                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    cGACAGCTTTTGCTCCTTCTTTATGCTGACCGCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAg                                                                                                                                                                                                                                             <  1:768573/250‑1 (MQ=255)          ttGCTCCTTCTTTATGCTGACCGCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAg                                                                                                                                                                                                                                   <  1:618627/251‑1 (MQ=255)                              ccGCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAg                                                                                                                                                                                                                                   >  1:326509/1‑231 (MQ=255)                                gCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAgg                                                                                                                                                                                                                                               >  1:750910/1‑217 (MQ=255)                                gCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAAtg                                                                                                                                                                                                                 >  1:540202/1‑247 (MQ=255)                                     ataCAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAGTTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAg                                                                                                                                                                                                                                             >  1:442660/1‑214 (MQ=255)                                     ataCAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAg                                                                                                                                                                                                                                   <  1:672940/224‑1 (MQ=255)                                                               gTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTaaa                                                                                                                                                                                                        <  1:277761/225‑1 (MQ=255)                                                                 agagAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAg                                                                                                                                                                                                                                   <  1:899242/196‑1 (MQ=255)                                                                                   aGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAg                                                                                                                                                           >  1:749616/1‑250 (MQ=255)                                                                                            tGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTc                                                                                                                                                 <  1:520127/251‑1 (MQ=255)                                                                                                                           aaaCTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAaac                                                                                                                  <  1:619994/251‑1 (MQ=255)                                                                                                                                ccTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATcc                                                                                                                                 >  1:616631/1‑231 (MQ=255)                                                                                                                                   tttAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCt                                                                                                          <  1:465817/251‑1 (MQ=255)                                                                                                                                          gTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTc                                                                                                    >  1:751683/1‑250 (MQ=255)                                                                                                                                            tCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAAttttt                                                                                                                                                                                                                     <  1:694270/135‑1 (MQ=255)                                                                                                                                               ttATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTaa                                                                                                                                                                                                                   >  1:607195/1‑134 (MQ=255)                                                                                                                                               ttATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAAtgt                                                                                                                                                                                                                <  1:795251/137‑1 (MQ=255)                                                                                                                                                      aTGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGc                                                                                                                                           <  1:386589/199‑1 (MQ=255)                                                                                                                                                      aTGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAaac                                                                                                                  >  1:460829/1‑224 (MQ=255)                                                                                                                                                       tGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGc                                                                                      <  1:665974/251‑1 (MQ=255)                                                                                                                                                             aTTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAgg                                                                                                                                                                                                                                               >  1:110611/1‑92 (MQ=255)                                                                                                                                                                        tatTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATc                                                                                                                                  <  1:75361/190‑1 (MQ=255)                                                                                                                                                                                       aaTATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTc                                                       <  1:64375/250‑1 (MQ=255)                                                                                                                                                                                                         gTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAg                                                                                                                                                                                                                                             >  1:76040/1‑50 (MQ=255)                                                                                                                                                                                                           cTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCAc                                                                                                 >  1:723400/1‑188 (MQ=255)                                                                                                                                                                                                                         gCAAATAATTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGAttt                          <  1:619433/245‑1 (MQ=255)                                                                                                                                                                                                                            aataatTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAg                                                                                                                                                                                                                                   >  1:809671/1‑41 (MQ=255)                                                                                                                                                                                                                            aataatTATCAGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAgg                  >  1:735571/1‑250 (MQ=255)                                                                                                                                                                                                                             ataatTATCAGTTTAAATTGGTCGTAGGAGTGAATaaa                                                                                                                                                                                                                                     <  1:143562/38‑1 (MQ=255)                                                                                                                                                                                                                                      aGTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTCGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAGGCAAACCAAGc        >  1:235653/1‑250 (MQ=255)                                                                                                                                                                                                                                       gTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTtaat                                                                                                                                                               <  1:570392/98‑1 (MQ=255)                                                                                                                                                                                                                                       gTTTAAATTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGcc                                                                       <  1:238525/186‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAGGCAAACCAAGCCTTccc  <  1:328431/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTTGGTCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAGGCAAACCAAGCCTTcc   >  1:294465/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    CGACAGCTTTTGCTCCTTCTTTATGCTGACCGCTATATACAGTGATGTCGGCGAAAGCGGCAGTAGAGAAAGCTAACGCAGTAGCAAGAGCTGCAAGTTTGAATTGATTAATCTTCATTTGAAAACTCCTTTTAAATGTTCTTTATACGATGGTTTATTCTGCAACATATTAAAATTGCATGAATATTTTTATTCTAAAAGTCTAATTTCCGAAATGCAAATAATTATCAGTTTAAATTGGCCGTAGGAGTGAATAAAAGTGCGGTGAATTTTTAATGTGTTTTAAATTTGTTTAATAAAAACACTTGAAAAATAACCGCACTTTAATAAAGCAAATTAGTCTTTCGCAGGATAATCCCACCAAATATCAAACAACTCGCTCACTTCCACTTGTTGTAAGCCGTTTTGGGTTAGCCAGTTTTTCACTAATTCTTGGTGAGACGCGTCGCATTTGCCGATTTTCTCCAGGCAAACCAAGCCTTCCC  >  1/605644‑606128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑