Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	369	T→A	intergenic (–/+2061)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	369	0	T	A	100.0%	69.1 / NA	32	intergenic (–/+2061)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/18);  total (14/18)

TTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATATTTGTACTATTT  >  1/216‑603                                                                                                                                                          |                                                                                                                                                                                                                                           ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                            <  1:656074/247‑1 (MQ=11) ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                            <  1:45851/247‑1 (MQ=11)   cttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGAt                                                                                                                                                 >  1:65448/2‑243 (MQ=11)                              ttGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGAttt                                                                                                              >  1:49810/1‑251 (MQ=255)                                gCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTag                                                                                                                    <  1:634013/243‑1 (MQ=255)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTagag                                                                                                                                                                   <  1:663032/186‑1 (MQ=11)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                                                                  >  1:372580/1‑235 (MQ=11)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCgg                                                                                                  >  1:68208/1‑251 (MQ=255)                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAgaga                                                                                                                                                                  >  1:23072/1‑182 (MQ=11)                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCAtgt                                                                                             >  1:617054/1‑251 (MQ=255)                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                                                                       >  1:862477/1‑251 (MQ=255)                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                                                                       >  1:813220/1‑251 (MQ=255)                                                          gtgtTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAAcc                                                                                   >  1:23451/1‑250 (MQ=255)                                                              tgtCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                            <  1:679958/189‑1 (MQ=11)                                                                tctcGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGcc                                                                             <  1:473612/250‑1 (MQ=255)                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACgg                                                                                                                             <  1:565227/191‑1 (MQ=11)                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGcc                                                                 <  1:104007/251‑1 (MQ=255)                                                                             aGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                                                          >  1:373634/1‑208 (MQ=11)                                                                                  aGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGt                                                          >  1:153904/1‑251 (MQ=255)                                                                                           cgTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCt                                                 <  1:853795/250‑1 (MQ=255)                                                                                            gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                                                                  <  1:232723/185‑1 (MQ=11)                                                                                            gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTa                                                >  1:604737/1‑251 (MQ=255)                                                                                                gATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                                                          >  1:31273/1‑189 (MQ=11)                                                                                                 aTTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGcc                                            <  1:765734/250‑1 (MQ=255)                                                                                                                    aGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGAt                         <  1:598807/250‑1 (MQ=16)                                                                                                                    aGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGAt                         <  1:755772/250‑1 (MQ=16)                                                                                                                         aaGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGc                    <  1:102854/250‑1 (MQ=16)                                                                                                                              tttGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAata              <  1:372098/251‑1 (MQ=16)                                                                                                                                  tactggAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATATTTg          <  1:147731/246‑1 (MQ=16)                                                                                                                                     tggAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATATTTGTAc       <  1:146265/249‑1 (MQ=18)                                                                                                                                          ggCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATATTTGTACTAttt  >  1:534402/1‑251 (MQ=18)                                                                                                                                                    aaGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACga                                   <  1:194359/208‑1 (MQ=18)                                                                                                                                                          |                                                                                                                                                                                                                                           TTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATATTTGTACTATTT  >  1/216‑603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑