Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	567,047	T→C	intergenic (+203630/‑4972)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	567,047	0	T	C	100.0%	182.4 / NA	61	intergenic (+203630/‑4972)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/30);  total (31/30)

AAGGAAAGATCGACGTGCGGTGACGCAAAGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCTACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCGCTGCTTAATTCA  >  1/566812‑567284                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              aaggaaAGATCGACGTGCGGTGACGCAAAGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCg                                                                                                                                                                                                                                >  1:134060/1‑251 (MQ=255)   ggaaAGATCGACGTGCGGTGACGCAAAGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTg                                                                                                                                                                                                                              >  1:676510/1‑251 (MQ=255)      aaGATCGACGTGCGGTGACGCAAAGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGAtt                                                                                                                                                                                                                           <  1:333944/251‑1 (MQ=255)                          cAAAGGCACTCCGGCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAata                                                                                                                                                                                                        <  1:858129/250‑1 (MQ=255)                            aaGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATc                                                                                                                                                                                                      >  1:391725/1‑250 (MQ=255)                              ggCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCcg                                                                                                                                                                                                    >  1:589307/1‑250 (MQ=255)                                cACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCcgcg                                                                                                                                                                                                  <  1:224289/250‑1 (MQ=255)                                                      tCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAgg                                                                                                                                                                           <  1:543727/251‑1 (MQ=255)                                                      tCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATCGCGCCAATTTTTCTAAAgg                                                                                                                                                                           <  1:425348/251‑1 (MQ=255)                                                            gggCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAGTGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCggg                                                                                                                                                                     >  1:450776/1‑251 (MQ=255)                                                               cAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGAt                                                                                                                                                                   <  1:75625/250‑1 (MQ=255)                                                                 atatTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAAt                                                                                                                                                                                                                  >  1:739061/1‑201 (MQ=255)                                                                   atTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTg                                                                                                                                                              <  1:676485/251‑1 (MQ=255)                                                                      ggCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGc                                                                                                                                                                                                                                     <  1:879247/177‑1 (MQ=255)                                                                        cAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTg                                                                                                                                                         <  1:530890/251‑1 (MQ=255)                                                                              tGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAAt                                                                                                                                                    <  1:353627/250‑1 (MQ=255)                                                                                  tcggatcAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGccc                                                                                                                                               <  1:568534/251‑1 (MQ=255)                                                                                     gatcAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAgg                                                                                                                                            >  1:700186/1‑251 (MQ=255)                                                                                           aaaaTAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGatgatg                                                                                                                                      <  1:315533/251‑1 (MQ=255)                                                                                                        aTTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATcc                                                                                                                          >  1:799436/1‑250 (MQ=255)                                                                                                                  aTCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCttt                                                                                                               >  1:349877/1‑251 (MQ=255)                                                                                                                  aTCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCttt                                                                                                               >  1:774548/1‑251 (MQ=255)                                                                                                                  aTCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCttt                                                                                                               >  1:461265/1‑251 (MQ=255)                                                                                                                  aTCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCttt                                                                                                               >  1:47565/1‑251 (MQ=255)                                                                                                                  aTCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCttt                                                                                                               >  1:257600/1‑251 (MQ=255)                                                                                                                                gcgAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAAt                                                                                                                                                    <  1:869321/200‑1 (MQ=255)                                                                                                                                   aaaTACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAga                                                                                               >  1:310603/1‑250 (MQ=255)                                                                                                                                   aaaTACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAga                                                                                               <  1:654987/250‑1 (MQ=255)                                                                                                                                   aaaTACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAga                                                                                               <  1:123998/250‑1 (MQ=255)                                                                                                                                                     ggCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGGCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGAtgatga                                                                                                                                     <  1:57432/194‑1 (MQ=255)                                                                                                                                                            cTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGt                                                                                                    >  1:470626/1‑220 (MQ=255)                                                                                                                                                                 aTTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCaa                                                                >  1:214784/1‑251 (MQ=255)                                                                                                                                                                             ttgCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAg                                                    >  1:830638/1‑251 (MQ=255)                                                                                                                                                                               gCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGtt                                                  >  1:112808/1‑251 (MQ=255)                                                                                                                                                                                 aCGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTAc                                                <  1:793273/251‑1 (MQ=255)                                                                                                                                                                                 aCGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTAc                                                <  1:740677/251‑1 (MQ=255)                                                                                                                                                                                    cgTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAAt                                                                                                                                                    <  1:665478/148‑1 (MQ=255)                                                                                                                                                                                    cgTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCg                                              <  1:610396/250‑1 (MQ=255)                                                                                                                                                                                    cgTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCg                                              <  1:71775/250‑1 (MQ=255)                                                                                                                                                                                        cATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGt                                                                                                                                                                                                 >  1:773506/1‑99 (MQ=255)                                                                                                                                                                                        cATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAgg                                                                                                                                                                           <  1:92161/121‑1 (MQ=255)                                                                                                                                                                                        cATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGatgatg                                                                                                                                      >  1:422207/1‑158 (MQ=255)                                                                                                                                                                                        cATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAg                                                    <  1:812365/240‑1 (MQ=255)                                                                                                                                                                                        cATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGa                                         >  1:483637/1‑251 (MQ=255)                                                                                                                                                                                                     cgTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAg                            <  1:891490/251‑1 (MQ=255)                                                                                                                                                                                                       ttttCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGTCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACt                                                                                    >  1:285371/1‑193 (MQ=255)                                                                                                                                                                                                        tttCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAAt                         <  1:80482/251‑1 (MQ=255)                                                                                                                                                                                                        tttCCATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTCCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAAt                         >  1:196223/1‑251 (MQ=255)                                                                                                                                                                                                           ccATTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTg                       >  1:759318/1‑250 (MQ=255)                                                                                                                                                                                                             aTTCTGTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCg                    <  1:257112/251‑1 (MQ=255)                                                                                                                                                                                                                  gTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGAt                >  1:313562/1‑250 (MQ=255)                                                                                                                                                                                                                  gTCCCCCAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGAt                >  1:813903/1‑250 (MQ=255)                                                                                                                                                                                                                     ccccAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCg              >  1:352474/1‑249 (MQ=255)                                                                                                                                                                                                                        cAAATGGACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCgctg           >  1:811734/1‑249 (MQ=255)                                                                                                                                                                                                                              gACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCGGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGAGAAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGTTAAAGATGATAAAACCTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTCCCGCCTGAAATT‑AAAAATAGAATTGCCGTGATCGCTGCTTAATTc   <  1:481564/250‑1 (MQ=255)                                                                                                                                                                                                                              gACTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACC‑AATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCGCTGCTTAATTc   <  1:529349/250‑1 (MQ=255)                                                                                                                                                                                                                               aCTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCGCTGCTTAATTc   <  1:386041/250‑1 (MQ=255)                                                                                                                                                                                                                               aCTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCGCTGCTTAATTCa  >  1:673900/1‑251 (MQ=255)                                                                                                                                                                                                                                cTCAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAga                                                                                               >  1:9573/1‑157 (MQ=255)                                                                                                                                                                                                                                  cAAATGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCg                                              <  1:719883/204‑1 (MQ=255)                                                                                                                                                                                                                                     aTGCTGCCACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATc               >  1:898679/1‑232 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              AAGGAAAGATCGACGTGCGGTGACGCAAAGGCACTCCGCCACGCCAGATTTTTTCCTGTGGGCAATATTGGCAATAGTGGATCGGATCAAAAAATAACGGCTTATTTGGCGGCATCCAGATTTGTCCGCGAAATACCCCGCCCAAACCGGCAATTCTTTTATTCTGAATTTGTTGCACGCGTCCATGTAAATTGCGCGTTTTCCATTCTGTCCCCCAAATGGACTCAAATGCTGCTACGGTTTTGCTGTCGTGATTGCCGTGAATAAACCAAATATCCGCGTATTGCGCCAATTTTTCTAAAGGTTCGGGATTGGTGAGTTGTAAATCGCCCAGGATGATGAGCGCCACATCCCGGCGTTCTTTCAGAAAAGGGTAAAGATGATCAAAACTCCCGTGCGGGTCGCCTGCAAATAAAAGCATAGTTACCGCCTGAAATTAAAAAATAGAATTGCCGTGATCGCTGCTTAATTCA  >  1/566812‑567284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑