BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	42,704	G→A	intergenic (+38097/+87801)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	42,704	0	G	A	100.0%	314.1 / NA	104	intergenic (+38097/+87801)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/72); total (32/72)

Only 100 of 104 total aligned reads displayed.

CATGAAACCGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCGGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCACCTAAATCTTCAACGAAAG  >  1/42459‑42943

cATGAAACCGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGtttt                                                                                                                                                                                                                                                      >  1:25253/1‑251 (MQ=255)

     aaCCGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTATGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAtg                                                                                                                                                                                                                                                 <  1:427228/251‑1 (MQ=255)

     aaCCGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAtg                                                                                                                                                                                                                                                 <  1:416631/251‑1 (MQ=255)

        cGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATgtgt                                                                                                                                                                                                                                              >  1:472904/1‑251 (MQ=255)

                agaagaTAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTAc                                                                                                                                                                                                                                       <  1:715967/250‑1 (MQ=255)

                    gaTAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGtttttt                                                                                                                                                                                                                                         <  1:180143/244‑1 (MQ=255)

                            ccGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGGCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAg                                                                                                                                                                                                                          <  1:146581/251‑1 (MQ=255)

                             cGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGa                                                                                                                                                                                                                         <  1:41376/251‑1 (MQ=255)

                              gTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCAGGAATGTAGaa                                                                                                                                                                                                                        >  1:366352/1‑251 (MQ=255)

                               tCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCggggaaggagaaa                                                                                                                                                                                                                       >  1:324928/1‑241 (MQ=255)

                                     gCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCGGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGGAGAATC‑ACCaa                                                                                                                                                                                                                >  1:427101/1‑250 (MQ=255)

                                     gCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGATGTAGaaaaaccc                                                                                                                                                                                                                  >  1:506811/1‑244 (MQ=255)

                                     gCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGaaataccaa                                                                                                                                                                                                                 >  1:560478/1‑244 (MQ=255)

                                     gCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGaaacaacaa                                                                                                                                                                                                                 >  1:876432/1‑244 (MQ=255)

                                     gCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGCGATGAAGAATC‑aacaa                                                                                                                                                                                                                >  1:89372/1‑247 (MQ=255)

                                          aCCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGATGTAGAATcttgttctttt                                                                                                                                                                                                            >  1:793245/1‑241 (MQ=255)

                                               cggcggAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAtg                                                                                                                                                                                                                                                 <  1:547009/209‑1 (MQ=255)

                                                      aCCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGAAAAGTAGAAAC‑ACCAA‑AAAAAAATTacgagggg                                                                                                                                                                                              >  1:584552/1‑244 (MQ=255)

                                                          aGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGttt                                                                                                                                                                                                                                                       >  1:191671/1‑192 (MQ=255)

                                                            cAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGtgat‑g                                                                                                                                                                                         <  1:19765/248‑1 (MQ=255)

                                                            cAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑Gaaa                                                                                                                                                                                      <  1:755840/251‑1 (MQ=255)

                                                            cAAGGCTTTCCAGCCATAACTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑Gaaa                                                                                                                                                                                      <  1:423655/251‑1 (MQ=255)

                                                              aGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTTATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGATTTTTAc                                                                                                                                                                                                                                       <  1:446863/204‑1 (MQ=255)

                                                                gCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGCATGGAGAAAA‑AACAG‑aaaaaaaatattggggggtggaaaaag                                                                                                                                                                                    >  1:122741/1‑230 (MQ=255)

                                                                        gCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCAGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAC‑ACCAG‑AAAAAAATTAAGC‑gggggtggaaaaagcctaaaat                                                                                                                                                                           >  1:427034/1‑231 (MQ=255)

                                                                         ccATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAAtt                                                                                                                                                                          <  1:765953/250‑1 (MQ=255)

                                                                         ccATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAAtt                                                                                                                                                                          <  1:862252/250‑1 (MQ=255)

                                                                             aGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCGCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGGAGAAAA‑ACCAA‑AAAAAAATTatgc                                                                                                                                                                                                  >  1:719271/1‑221 (MQ=255)

                                                                              gCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGATGTAAAATC‑ACCAG‑AAAAAAATTAGGC‑GGTGGT‑GAAAAAAGCCTAAATTAGa‑aaa                                                                                                                                                                   >  1:342511/1‑251 (MQ=255)

                                                                               cTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAtg                                                                                                                                                                                                                                                 <  1:698667/177‑1 (MQ=255)

                                                                               cTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAtg                                                                                                                                                                                                                                                 <  1:703326/177‑1 (MQ=255)

                                                                                 aTTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGtt                                                                                                                                                                                                                                             <  1:543633/179‑1 (MQ=255)

                                                                                      cGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGAAGGAGAAAA‑AACAA‑AAAAAAATTAAGC‑GGGGGT‑GAAAACCGCCCAAATTaaaaaaaaaaatat                                                                                                                                                            >  1:170521/1‑238 (MQ=255)

                                                                                      cGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAattat                                                                                                                                                           <  1:712010/251‑1 (MQ=255)

                                                                                        tttGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTattg                                                                                                                                                         <  1:90652/251‑1 (MQ=255)

                                                                                              gtCATGTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAgtgt                                                                                                                                                                                                                            <  1:390163/183‑1 (MQ=255)

                                                                                               tCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGttt                                                                                                                                                 <  1:359888/251‑1 (MQ=255)

                                                                                                       aaTTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGt                                                                                                                                          <  1:196539/250‑1 (MQ=255)

                                                                                                         ttGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTaat                                                                                                                                       <  1:589709/251‑1 (MQ=255)

                                                                                                         ttGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTaat                                                                                                                                       <  1:523716/251‑1 (MQ=255)

                                                                                                                   gTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGAAGGAGAAAA‑ACCAG‑AAAAAAATTAAGG‑GGTGAT‑GGAAAACGCCCAAAATAGC‑AAAACAAATATTTA‑TTTTTTTTAAAGTAATCAAACGCTGa                                                                                                                             >  1:812389/1‑251 (MQ=255)

                                                                                                                          aCGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACt                                                                                                                      <  1:149679/251‑1 (MQ=255)

                                                                                                                            gggTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAA‑ACCAG‑AAAAAAATTATGC‑GGGGGT‑GAAAAACGCCCAAAATAGA‑AAAACAAATATTTA‑TTTTTTTTTAAGAAATCAAACACTGATTGAAAtgt                                                                                                                    >  1:219073/1‑251 (MQ=255)

                                                                                                                             ggTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGGAGAATA‑ACCCG‑AAAAAAATTATGC‑GGTGGT‑GGAAAACGCCCCAAATAGC‑AAAACAAATATTGA‑TTGTTTTTTAAGTaatcaaaaactgatttaaatgtt                                                                                                                   >  1:166012/1‑234 (MQ=255)

                                                                                                                                 aCATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAAAGTAGAATC‑ACCAGAAAAAAATTTA‑GC‑GGGGGT‑GGAAAACGCCCAAAATAGC‑AAAAAAAATATTTA‑TTTTTTTTAAAGGAATCAAACGCTGATTTAACTGTtgtgg                                                                                                               >  1:129984/1‑250 (MQ=255)

                                                                                                                                  cATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTAc                                                                                                                                                                                                                                       <  1:790245/136‑1 (MQ=255)

                                                                                                                                  cATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTaaa                                                                                                                                                                            <  1:213869/191‑1 (MQ=255)

                                                                                                                                   aTTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGAtt                                                                                                             <  1:382105/251‑1 (MQ=255)

                                                                                                                                   aTTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGAtt                                                                                                             <  1:693546/251‑1 (MQ=255)

                                                                                                                                                    aagagaTTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGTATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCa                                                                                             <  1:818015/249‑1 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAAtt                                                                                                                                                                          <  1:658626/168‑1 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAatta                                                                                                                                                            <  1:487021/181‑1 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAC‑ACCAG‑AAAAAATTTAAGG‑GGGGGT‑GGAAAACGCCCAAATTAACAAAATC‑ATTATTTT‑TTTTTTTTTAAGTAATAAATACCTGCTTGAACTGTTTTGAtttttttttcttttttttcccggatt                                                                                     >  1:231019/1‑231 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAC‑ACCAG‑AAAAAAATTAGGC‑GGGGGT‑GAAAAACACCCAAAATAAC‑AAAACAAATATTTA‑TTTTTTTTTAAGTAATAAATAGCTGATTGTAATGTTGTGGtttttttttcttttttatcccggatt                                                                                     >  1:541499/1‑231 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGGAGAATCAACCCA‑AAAAAATTT‑GGG‑GGGGGT‑GAAAACCGCCCAAAATAAC‑AAAAAAAATATTTA‑TTTTTTTTTACGTAATCAATACCTCATTTAACTGTTTTGGtttttttttcttttttttccggactt                                                                                     >  1:668428/1‑231 (MQ=255)

                                                                                                                                                           tttttAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTGCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑Gaaa                                                                                                                                                                                      <  1:13342/156‑1 (MQ=255)

                                                                                                                                                                  gCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAAc                                                                                                                                                                                    <  1:17436/151‑1 (MQ=255)

                                                                                                                                                                     gtgtACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAAt                                                                                                                                                                                                                                                  >  1:753110/1‑90 (MQ=255)

                                                                                                                                                                     gtgtACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGGAAGTAGAATC‑ACCAC‑AAAAAAATTAAGCGGGGGGG‑GAAAACC‑CCTAAATTAGT‑AAATAAATTATTTA‑TTGTTTTTAACGTAAACAAACTCTGATTGAAATGTTGTGATTTTTTTATc‑ttctttctcccgactttttttatcga                                                                          >  1:585085/1‑230 (MQ=255)

                                                                                                                                                                     gtgtACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAA‑ACCAG‑AAAAAAATTTGGC‑GGGGGT‑GAAAAACGCCTAAATTAAC‑AAAAAAAATATTTATTTTTTTTGAA‑GGAATCAATAGCTGATTGAACTGTTGTGATTTTTTTAGC‑TTCTTTCT‑CCGGAAtttttatttcga                                                                         >  1:782408/1‑242 (MQ=255)

                                                                                                                                                                       gtACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGAt                                                                                                                            <  1:477678/200‑1 (MQ=255)

                                                                                                                                                                         aCAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑ttc                                                                    <  1:13866/251‑1 (MQ=255)

                                                                                                                                                                         aCAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTCGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑ttc                                                                    <  1:438207/251‑1 (MQ=255)

                                                                                                                                                                            aGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACt                                                                                                                      <  1:190630/201‑1 (MQ=255)

                                                                                                                                                                             ggCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGAtt                                                                                                             <  1:624879/209‑1 (MQ=255)

                                                                                                                                                                              gCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACg                                                                                                                                           <  1:304710/178‑1 (MQ=255)

                                                                                                                                                                               cTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑ttcttcttc                                                              <  1:511323/251‑1 (MQ=255)

                                                                                                                                                                                    aaaTTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAg                                                         <  1:411038/251‑1 (MQ=255)

                                                                                                                                                                                    aaaTTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAg                                                         <  1:535559/251‑1 (MQ=255)

                                                                                                                                                                                            cgcCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑cc                                                <  1:367584/251‑1 (MQ=255)

                                                                                                                                                                                              ccAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAg                                                         <  1:888249/241‑1 (MQ=255)

                                                                                                                                                                                                 gTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACt                                                                                                                      <  1:235213/180‑1 (MQ=255)

                                                                                                                                                                                                 gTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAAATTTGGC‑GGGGGT‑GAAAAACGCCCAAATTAAC‑AAAACAATTATTTA‑TTGTTTTTTAAGGAATCAATCGCTGGTTTAACTGTTGTGATTTTTTTATC‑TTTTTTTT‑CCGGAATTTCGatat                                                                            >  1:152651/1‑220 (MQ=255)

                                                                                                                                                                                                 gTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAA‑ACCAG‑AAAAAAATTTGGC‑GGGGGTGGAAAACC‑CCCAAAATAAC‑AAAAAAAATATTTA‑TTGTTTTTTAAGGAATAAAAAGCTGATTGAAATGTTGTGATTTTTTTTGC‑TTTTTTTT‑CCGGAATTTTGATATTGAA‑TTTTTTTTTTTAAACCATCA‑CCaaatt                                           >  1:413873/1‑248 (MQ=255)

                                                                                                                                                                                                   cTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAAc                                                                                                                                            <  1:714531/156‑1 (MQ=255)

                                                                                                                                                                                                    tACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAAc                                        <  1:33434/251‑1 (MQ=255)

                                                                                                                                                                                                     aCTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑c                                                 <  1:507956/241‑1 (MQ=255)

                                                                                                                                                                                                      cTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAg                                      <  1:699179/251‑1 (MQ=255)

                                                                                                                                                                                                      cTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAg                                      <  1:691344/251‑1 (MQ=255)

                                                                                                                                                                                                      cTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAg                                      <  1:556596/251‑1 (MQ=255)

                                                                                                                                                                                                        gCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGCGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCCG‑AAAAAAATTTGGC‑GGGGAA‑GAAAATCGCCCCAATTTGC‑AAAAAAATTATTTA‑TTGTTTTTTACGTAAAAAAACGCTGATTGAAATGTTGTGGTTTTTTTAGA‑TTCTTCAT‑CCCCAAATTCGTTATCTAA‑TTTTTTTTCTAAAACCATCA‑CCCATTTAACAgca                                    >  1:602751/1‑249 (MQ=255)

                                                                                                                                                                                                           ttCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCt                                 <  1:148854/251‑1 (MQ=255)

                                                                                                                                                                                                           ttCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCt                                 <  1:605294/251‑1 (MQ=255)

                                                                                                                                                                                                                ggAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAg                                      <  1:608350/241‑1 (MQ=255)

                                                                                                                                                                                                                ggAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAg                             <  1:783819/250‑1 (MQ=255)

                                                                                                                                                                                                                ggAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAg                             <  1:112670/250‑1 (MQ=255)

                                                                                                                                                                                                                ggAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAg                             <  1:426880/250‑1 (MQ=255)

                                                                                                                                                                                                                 gAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGa                            <  1:375176/250‑1 (MQ=255)

                                                                                                                                                                                                                  aaaaTTTCAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAAt                          <  1:47137/251‑1 (MQ=255)

                                                                                                                                                                                                                         cAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCAcc                   <  1:164023/251‑1 (MQ=255)

                                                                                                                                                                                                                         cAATAAGCAAAGCACAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCAcc                   <  1:206011/251‑1 (MQ=255)

                                                                                                                                                                                                                             aagcaaagcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAAc                                        <  1:362921/226‑1 (MQ=255)

                                                                                                                                                                                                                               gcaaagcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGc                                                        <  1:366612/209‑1 (MQ=255)

                                                                                                                                                                                                                               gcaaagcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAAAA‑ACCAG‑AAAAAAATTTGGC‑GGGGGT‑GAAAAACGCCCAAATTAAC‑AAAAAAAATATTTA‑TTTTTTTTTACGTAATCAATCGCTGATTTAAATGTTGTGGTTTTTTTTGCTTTTTTTTT‑CCGG‑ATTTTGATATCGAATTTTTTTTTTTAAACCCA‑CACCCAATT‑AACAGTATCTAAAAAATCCCCACCCaaaaa             >  1:432844/1‑249 (MQ=255)

                                                                                                                                                                                                                                caaagcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCACCTAAATCt            <  1:344120/251‑1 (MQ=255)

                                                                                                                                                                                                                                  aagcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAAc                                                                                                                                                                                    <  1:789892/87‑1 (MQ=255)

                                                                                                                                                                                                                                    gcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAg                                                         <  1:220973/203‑1 (MQ=255)

                                                                                                                                                                                                                                    gcaCAGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGaa                           <  1:89130/232‑1 (MQ=255)

                                                                                                                                                                                                                                       caGAAAGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAAAGTAGAATC‑ACCCG‑AAAAAAATTATGC‑GGGGATGGAAAACC‑CCCAAAATATC‑AAAACAAATATTTA‑TTTTTTTTGACGTAATCAATCCCTGATTGAAATTTTTTGGTTTTTTTTGC‑TTTTTTATCCCGGCATTT‑TATATTTAA‑TTTTTTTTTTTAAACCATCA‑CCAATTCAACAAAATCTAAAAAAACCCCACCCAAATATTCAAcca     >  1:839635/1‑249 (MQ=255)

                                                                                                                                                                                                                                          aaaGTGCGGTCAGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCACCTAAATCTTCAACGAAAg  <  1:488459/251‑1 (MQ=255)

CATGAAACCGAGAATGAGAAGATAGAAACCGTCCATGGCATAACCCACGGCGGAACCCAGCAAGGCTTTCCAGCCATAGCTATTTACGTTTGTTGTCATTTTGAATTGTCCTTCTGTTACTCACGGGTAACATTTAAAGTTAGAAGAGAAGAGATTTTTTAGGCTGTGTACAAGGCTAAAAAATTGTGCGCCAGTCTACTGCTTTCAGGGAAAATTTCAATAAGCAAAGCACAGAAAGTGCGGTCGGTTTTGAATGTGTTTTTTACGGCGGGAATGTAGAATC‑ACCAG‑AAAAAATTTAGGC‑GGTGAT‑GAAAACCGCCTAAATTAGC‑AAATCAATTATTGA‑TTGTTTTGAACGTAATCAATCGCTGATTGAACTGTTGTGATTTTTTCAGC‑TTCTTCAT‑CCGGAATTTCGATATCGAA‑TTCTTCTTCTAAAGCCATCA‑CCAATTCAACAGTATCTAAAGAATCCGCACCTAAATCTTCAACGAAAG  >  1/42459‑42943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`