Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	545,602	T→C	intergenic (+182185/‑26417)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	545,602	0	T	C	100.0%	226.9 / NA	75	intergenic (+182185/‑26417)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/28);  total (47/28)

CTTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATTACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGGCGCTGCAACACTTGA  >  1/545358‑545848                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ctTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGTGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCActc                                                                                                                                                                                                                                                    <  1:450398/249‑1 (MQ=255) ctTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCAct                                                                                                                                                                                                                                                     >  1:260086/1‑248 (MQ=255) ctTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCAct                                                                                                                                                                                                                                                     >  1:854288/1‑248 (MQ=255) ctTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCAct                                                                                                                                                                                                                                                     >  1:531759/1‑248 (MQ=255) ctTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCAct                                                                                                                                                                                                                                                     >  1:740982/1‑248 (MQ=255)   ttCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGcc                                                                                                                                                                                                                                                <  1:722340/251‑1 (MQ=255)         ccGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCcttcttc                                                                                                                                                                                                                                          <  1:711727/251‑1 (MQ=255)            cgcAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCttt                                                                                                                                                                                                                                       <  1:278296/251‑1 (MQ=255)                aaaTGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCcttc                                                                                                                                                                                                                                             <  1:218950/241‑1 (MQ=255)                          ggTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTa                                                                                                                                                                                                                         >  1:604407/1‑251 (MQ=255)                          ggTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTa                                                                                                                                                                                                                         >  1:326466/1‑251 (MQ=255)                             gtCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGATTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAaca                                                                                                                                                                                                                      >  1:447723/1‑251 (MQ=255)                               cTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCcttcttct                                                                                                                                                                                                                                         <  1:482769/230‑1 (MQ=255)                               cTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAacac                                                                                                                                                                                                                     >  1:123699/1‑250 (MQ=255)                                                                          ttCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCgg                                                                                                                                                                         >  1:300651/1‑251 (MQ=255)                                                                                                  gTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:498764/1‑251 (MQ=255)                                                                                                  gTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:468140/1‑251 (MQ=255)                                                                                                  gTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:342966/1‑251 (MQ=255)                                                                                                  gTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:773239/1‑251 (MQ=255)                                                                                                  gTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:157711/1‑251 (MQ=255)                                                                                                   tATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 <  1:671943/250‑1 (MQ=255)                                                                                                   tATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 <  1:215891/250‑1 (MQ=255)                                                                                                    aTTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGa                                                                                                                                                >  1:116919/1‑250 (MQ=255)                                                                                                       gACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCatat                                                                                                                                                       >  1:132055/1‑240 (MQ=255)                                                                                                           atatTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAAc                                                                                                                                                                                                          >  1:207905/1‑185 (MQ=255)                                                                                                           atatTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAAc                                                                                                                                                                 >  1:212917/1‑226 (MQ=255)                                                                                                              ttCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACgtt                                                                                                                                     <  1:533144/251‑1 (MQ=255)                                                                                                                 aTCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCatat                                                                                                                                                       <  1:300428/230‑1 (MQ=255)                                                                                                                 aTCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACgtttgt                                                                                                                                  >  1:673229/1‑251 (MQ=255)                                                                                                                 aTCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACgtttgt                                                                                                                                  >  1:24081/1‑251 (MQ=255)                                                                                                                     aaGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGttttt                                                                                                                              >  1:856752/1‑251 (MQ=255)                                                                                                                             ggCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCtt                                                                                                                      >  1:818089/1‑251 (MQ=255)                                                                                                                               cgcCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCatat                                                                                                                                                       >  1:451122/1‑216 (MQ=255)                                                                                                                                 cccACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCgg                                                                                                                  >  1:872246/1‑251 (MQ=255)                                                                                                                                    aCGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCa                                                                                                                >  1:722164/1‑250 (MQ=255)                                                                                                                                    aCGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCAc                                                                                                               <  1:4087/251‑1 (MQ=255)                                                                                                                                         atgcaatTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCa                                                                                                                                                     >  1:273116/1‑208 (MQ=255)                                                                                                                                         atgcaatTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCg                                                                                                           >  1:8329/1‑250 (MQ=255)                                                                                                                                         atgcaatTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTG‑TTTTCAACCCTTCCGGCACTTCGc                                                                                                          >  1:735038/1‑250 (MQ=255)                                                                                                                                           gcaatTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCacac                                                                                                                                        <  1:140620/219‑1 (MQ=255)                                                                                                                                              atTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAAc                                                                                                                          <  1:690600/230‑1 (MQ=255)                                                                                                                                                        cgcCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGc                                                                                            <  1:119490/250‑1 (MQ=255)                                                                                                                                                        cgcCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCt                                                                                           <  1:726735/251‑1 (MQ=255)                                                                                                                                                             aaCTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCgg                                                                                                                  >  1:297077/1‑223 (MQ=255)                                                                                                                                                                 tGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAAc                                                                                  <  1:35854/251‑1 (MQ=255)                                                                                                                                                                       gAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAAc                                                                                  <  1:484502/245‑1 (MQ=255)                                                                                                                                                                        aGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCca                                                                            >  1:628696/1‑250 (MQ=255)                                                                                                                                                                          cAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACaa                                                                         >  1:63754/1‑251 (MQ=255)                                                                                                                                                                            ggTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCa                                                                                                                                                     >  1:246857/1‑173 (MQ=255)                                                                                                                                                                            ggTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAAc                                                                                  >  1:167629/1‑240 (MQ=255)                                                                                                                                                                             gTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCCGCcttct                                                                                                                                                                                                                                            >  1:70119/1‑85 (MQ=255)                                                                                                                                                                              ttGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATgg                                                                      <  1:875357/250‑1 (MQ=255)                                                                                                                                                                                    gCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCAc                                                                                                               >  1:758560/1‑203 (MQ=255)                                                                                                                                                                                      gTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGc                                                                                                                                            <  1:817373/172‑1 (MQ=255)                                                                                                                                                                                                 cGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGt                                                  <  1:43849/251‑1 (MQ=255)                                                                                                                                                                                                     ggTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCg                                                                                                                                                 >  1:767723/1‑152 (MQ=255)                                                                                                                                                                                                      gTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAAC‑‑‑CCACAATGGCGGTTTTACCCACACCCCGTTCAcccca                                          >  1:97011/1‑248 (MQ=255)                                                                                                                                                                                                             ccGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGc                                      <  1:155381/251‑1 (MQ=255)                                                                                                                                                                                                                cgcCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCAc                                    >  1:184045/1‑250 (MQ=255)                                                                                                                                                                                                                cgcCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCAc                                    >  1:840343/1‑250 (MQ=255)                                                                                                                                                                                                                cgcCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCAc                                    >  1:440510/1‑250 (MQ=255)                                                                                                                                                                                                                cgcCCACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGGTCACCGATAAGcca                                    >  1:296614/1‑248 (MQ=255)                                                                                                                                                                                                                  cccACCATAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTAccc                                                           <  1:239112/225‑1 (MQ=255)                                                                                                                                                                                                                      ccaTAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGccc                                                                                                                                                                            >  1:258180/1‑108 (MQ=255)                                                                                                                                                                                                                      ccaTAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGc                                                                                            <  1:338912/188‑1 (MQ=255)                                                                                                                                                                                                                      ccaTAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTg                             >  1:550839/1‑251 (MQ=255)                                                                                                                                                                                                                        aTAGTGTGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCAc                                    <  1:807198/242‑1 (MQ=255)                                                                                                                                                                                                                           gtgtGAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACGCCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAAc                                                                                  <  1:26946/193‑1 (MQ=255)                                                                                                                                                                                                                              tgAATTTCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGt                     <  1:535681/251‑1 (MQ=255)                                                                                                                                                                                                                                  tttCATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACgg                 >  1:542987/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATCGATGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGgcgc              <  1:837071/251‑1 (MQ=255)                                                                                                                                                                                                                                          aTGAACAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGGCGCTGCaa         <  1:254513/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaCAAAATCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCa                                                                                                                                                     >  1:306236/1‑108 (MQ=255)                                                                                                                                                                                                                                                 aaaTCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGGCGCTGCAACACTTGa  >  1:466443/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aaaTCACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGGCGCTGCAACACTTGa  >  1:748609/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CTTTCAAACCGCGCAAAATGGCGATGGTGTCTTCCACGCTCGGTTCGCCCACAAACACTTTTTGGAAACGACGTTCAAGCGCCGCGTCTTTTTCAATGTATTGACGATATTCATCCAAGGTAGTGGCGCCCACGCAATGCAATTCACCGCGCGCCAAACTTGGTTTGAGCAGGTTGCCTGCGTCCATGGCGCCGTCGGTTTTACCCGCGCCCACCATAGTGTGAATTTCATCGATGAACAAAATTACTCTGCCTTCTTCTTTGGCGAGTTCGTTTAACACCGCTTTCAAACGTTCTTCAAATTCGCCGCGATATTTCGCCCCGGCGATTAACGCGCCCATATCAAGCGACAGCACACGTTTGTTTTTCAACCCTTCCGGCACTTCGCCGTTCACGATACGCTGTGCCAAACCTTCCACAATGGCGGTTTTACCCACACCCGGTTCACCGATAAGCACCGGATTGTTTTTGGTACGGCGCTGCAACACTTGA  >  1/545358‑545848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑