Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	541,354	G→A	intergenic (+177937/‑30665)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	541,354	0	G	A	100.0%	233.8 / NA	80	intergenic (+177937/‑30665)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/28);  total (52/28)

AGCGCCGCTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTGCGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTCCGCGTT  >  1/541117‑541590                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  aGCGCCGCTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAt                                                                                                                                                                                                                                      >  1:730957/1‑251 (MQ=255)   cgccgcTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAtt                                                                                                                                                                                                                                     <  1:116144/250‑1 (MQ=255)   cgccgcTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAt                                                                                                                                                                                                                                      <  1:839184/249‑1 (MQ=255)     ccgcTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAtctg                                                                                                                                                                                                                                   >  1:194031/1‑247 (MQ=255)           ccGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGGTTGGATTTCCGTACGCAGAATATAATTTTTCTCaaa                                                                                                                                                                                                                            >  1:434816/1‑251 (MQ=255)                      ggTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTacgc                                                                                                                                                                                                                                                <  1:751545/220‑1 (MQ=255)                                       gTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAt                                                                                                                                                                                                                                      >  1:46382/1‑213 (MQ=255)                                                cgcgTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAACGGGAATTTT‑TTTGGCTcccca                                                                                                                                                                                    >  1:434000/1‑249 (MQ=255)                                                    tAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATc                                                                                                                                                                                 <  1:871782/250‑1 (MQ=255)                                                     aGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATTCTCCACCTGCACCACGCAAAGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAAA‑GGAAATTTAtttctgttttctataaa                                                                                                                                                                               >  1:432494/1‑236 (MQ=255)                                                      gTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGCGGGA‑AAAC‑GGATtttttttggccccccaaacggg                                                                                                                                                                               >  1:327599/1‑233 (MQ=255)                                                        ttGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAA‑GGGAATTTATTCGGCTCCCAAAACGGttt                                                                                                                                                                            >  1:289566/1‑250 (MQ=255)                                                                   aCGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGTAAAAC‑GAAATTTT‑TTCTGCTccccaaacgggtttcccctgtttt                                                                                                                                                                 >  1:551934/1‑230 (MQ=255)                                                                   aCGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTc                                                                                                                                                                  <  1:146192/250‑1 (MQ=255)                                                                      aTTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGGATTTTTTTTGGCTccccaaaccgtttgcccctgtttacc                                                                                                                                                               >  1:310377/1‑227 (MQ=255)                                                                      aTTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAAtttt                                                                                                                                                                                                 <  1:624558/216‑1 (MQ=255)                                                                          cccaccTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCAc                                                                                                                                                          <  1:671619/250‑1 (MQ=255)                                                                                 gCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAA‑GGAATTTTTTTTCGCTCCCAAATCGGTTCGCCCCTGTTTACCCCCAccgtacc                                                                                                                                                    >  1:473861/1‑244 (MQ=255)                                                                                 gCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAAA‑GGGAATTTATTCGGCTCCCCAAACGGTTCCCCCCTGtttccccccaccttacc                                                                                                                                                    >  1:57886/1‑235 (MQ=255)                                                                                 gCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAAA‑GGAATTTTTTTTGGCTCCCCAAACGGTTCGCCCCTGTTCAcccc                                                                                                                                                             >  1:627548/1‑240 (MQ=255)                                                                                              aTTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGACACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACgg                                                                                                                                     <  1:153352/251‑1 (MQ=255)                                                                                                 cACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAt                                                                                                                                                                                                                                      <  1:377411/155‑1 (MQ=255)                                                                                                        tCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCAt                                                                                                                           <  1:722499/251‑1 (MQ=255)                                                                                                                       tGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAAA‑GGGAATTTATTCCGCTCCCCAAAAGGTTCTCCCCCGTTTACCCCCACGGTACCATCTTC‑TTTTACCGTCAGCAACATTTTTCCGTTTTTCGt                                                                                                            >  1:103440/1‑251 (MQ=255)                                                                                                                            ttCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGTAAAAG‑GGAATTTT‑TTCGGCTCCCCAAACGGTTCGCCCCTGTTTACCCCCCCGTTACCATCTTC‑TTTCACCGGCAGCAGCATTTTTCCGTTTTTCGGGCttt                                                                                                       >  1:173223/1‑250 (MQ=255)                                                                                                                                aaTAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGc                                                                                                   <  1:756257/251‑1 (MQ=255)                                                                                                                                 aTAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCg                                                                                                  <  1:549832/251‑1 (MQ=255)                                                                                                                                      ggACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGTAAAAG‑GGAATTTT‑TTCGGCTCCCAAAACGGGTCTCCCATGTTTACCCCCACGTTACCCTCTTT‑TTTTACGGTCAGCAGCATTTTGCCCGTTTGCGTGCTTACGGCGATgg                                                                                              >  1:158792/1‑249 (MQ=255)                                                                                                                                            aGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAttt                                                                                                                                                                                                                                    >  1:274631/1‑114 (MQ=255)                                                                                                                                                gttCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTTCGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTAACATCTTCTTTTCCCGG‑CCGCAGCATTTTTCCGTTTTTCGTGCtttccgcggttgtttttttattt                                                                                   >  1:849345/1‑230 (MQ=255)                                                                                                                                                gttCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGc                                                                                                          <  1:465211/228‑1 (MQ=255)                                                                                                                                                gttCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGG‑AAAA‑GGAATTTTTTTTGGCTCCCAAAACTGTTCGCCCATGTTTACCCCCCCGTTACCCTCTTC‑TTTCAACGTCAGCAGCATTTTGCCGTTTTTCGTGCTTTCGGCGCTGTC‑tttttttttt                                                                                  >  1:781099/1‑247 (MQ=255)                                                                                                                                                      caTTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCa                                                                                                                                                                       <  1:485309/162‑1 (MQ=255)                                                                                                                                                       aTTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGc                                                                           <  1:372439/251‑1 (MQ=255)                                                                                                                                                              gTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCCGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTTCCCTTTTGCGTGCTTACGGCg                                                                                                  >  1:26460/1‑222 (MQ=255)                                                                                                                                                               tttGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGc                                                                   <  1:562815/251‑1 (MQ=255)                                                                                                                                                                       tcctccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCCCACGTTACCATCTTC‑TTTCACCGTCAGCAGCATTTTGCCGTTTTTCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTTAGCCTTTGAGCGTCACGaa                                                           >  1:820226/1‑251 (MQ=255)                                                                                                                                                                       tcctccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAA‑GGAATTTTATTCGGCTCCCAAATCGGGTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGGAATGTC‑TTTTTTAt                                                                                    >  1:245067/1‑226 (MQ=255)                                                                                                                                                                        cctccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCCTGTTCACCCGCACGTTTCCATCTTC‑TTTTACCGCTAGCCGCATTTTGACGGTTTGCTTGGTTACGGCGATGTCTTTTTTTTTTGGTT‑AGCCTTTTTGCGTCACgtaa                                                          >  1:703598/1‑248 (MQ=255)                                                                                                                                                                        cctccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGaaa                                                          >  1:554669/1‑251 (MQ=255)                                                                                                                                                                        cctccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGaaa                                                          <  1:429680/251‑1 (MQ=255)                                                                                                                                                                           ccACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGGTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACgg                                                       >  1:624954/1‑251 (MQ=255)                                                                                                                                                                            cACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAAt                                                                                                                                                                                                                                      >  1:84397/1‑80 (MQ=255)                                                                                                                                                                            cACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCAt                                                                                                                                                  <  1:630065/161‑1 (MQ=255)                                                                                                                                                                            cACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCCCGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACggg                                                      >  1:440477/1‑251 (MQ=255)                                                                                                                                                                            cACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGcc                                                                          <  1:846444/231‑1 (MQ=255)                                                                                                                                                                                  caccacGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAAc                                                <  1:843847/251‑1 (MQ=255)                                                                                                                                                                                  caccacGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAAc                                                <  1:7135/251‑1 (MQ=255)                                                                                                                                                                                         cAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTTGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTTTTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTg                                          >  1:643289/1‑250 (MQ=255)                                                                                                                                                                                                    gCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCAt                                                                                                                           <  1:559663/159‑1 (MQ=255)                                                                                                                                                                                                    gCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGGGCGCCGTTGCca                               >  1:696359/1‑250 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAc                                                                                                                                                                                                          >  1:748968/1‑81 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCagcagc                                                                                                                             <  1:158722/156‑1 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGc                                                                                                   <  1:898169/182‑1 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGtt                                                                               >  1:870751/1‑201 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCcaca                             >  1:565804/1‑251 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGGTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGGTGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCcaca                             >  1:727593/1‑251 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAAACGGTTCTCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCCTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCcaca                             >  1:146801/1‑251 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAA‑GGGATTTTTTTTGGCTCCCCAATCGGTTTGCCCATGTTCACCCGCCCGTTACCATTTTC‑TTTTACGGTCAGCAGCATTTTGCCGTTTTGCGGGCTTTCGGCGACGTC‑TTTTTTATTGGTTAAGGCTTTTAGGGTCACGAAACGGGCGAAACAGCGGGCGCCGTTGCcaca                             >  1:336546/1‑251 (MQ=255)                                                                                                                                                                                                     cccATAGATACCGCGCTGCACAGCACCGTTTAGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAAt                                                                                                                                                                                                    >  1:799968/1‑86 (MQ=255)                                                                                                                                                                                                       cATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGGATTTTATTCCGCTCCCCAATCGGTTCGCCCACGTTCACCCGCACGTTAACATCTTC‑TTTCCCGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGCTGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCCAAACAGCGGGCGCCGTTGCCACAtt                           >  1:131805/1‑251 (MQ=255)                                                                                                                                                                                                       cATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTcagcag                                                                                                                              <  1:738395/153‑1 (MQ=255)                                                                                                                                                                                                       cATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTTCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACAtt                           >  1:361704/1‑251 (MQ=255)                                                                                                                                                                                                       cATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCtt                                                                        >  1:588760/1‑206 (MQ=255)                                                                                                                                                                                                              aCCGCGCTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCAc                    <  1:756091/251‑1 (MQ=255)                                                                                                                                                                                                                 gcgcTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACtt                  >  1:230829/1‑250 (MQ=255)                                                                                                                                                                                                                 gcgcTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCC‑TTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCg                >  1:613505/1‑251 (MQ=255)                                                                                                                                                                                                                    cTGCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACAt                            <  1:752452/237‑1 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCTGCGCCCAAATCGGTTCGCCCCTGTTTACCCACACGTTACCATCTTT‑TTTCACGGGCAGCAGCATTTTGCCCTTTTTCGTGCTTACGGCGATGTC‑TTTTTTTTTGGTTAAGCCTTTGAGCGTCCCGAAACGGGCGAAACAACGTGCGCCGTTGCCCCATTGCGCCACTTCGccg             >  1:71895/1‑248 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCCTGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTg             >  1:279399/1‑250 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAg                                              <  1:716572/217‑1 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTg             >  1:808876/1‑250 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTg             >  1:279636/1‑250 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGGTTTTCGTGCTTACGGCGATGTC‑TTTTTTTTTGGTTAAGCCTTTGAGCGTCACGAAACGGGCCAAACAGCGTGCGCCGGTGCCACATTGCGCCACTTCGCTg             >  1:20035/1‑250 (MQ=255)                                                                                                                                                                                                                      gCACAGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCCCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCCCGGTCCGCAGCATTTTTCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGCGCGCCGGTGCCCCATTGCGCCCCTTCGCTg             >  1:515628/1‑250 (MQ=255)                                                                                                                                                                                                                       cacaGCACCGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTT‑‑‑CCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTc        <  1:99560/251‑1 (MQ=255)                                                                                                                                                                                                                              ccGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTTCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCCTTGAGCGTCACCAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTCcgcg    >  1:20421/1‑251 (MQ=255)                                                                                                                                                                                                                              ccGTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTCcgcg    >  1:773289/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCg                                                                                                  >  1:562621/1‑156 (MQ=255)                                                                                                                                                                                                                                gTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAAACGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTTCGTGCTTTCGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTCCGCGtt  >  1:883341/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTTGGATTTCCGTACGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCCCCCAAATCGGGTTGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCCTTTTGCCGTTTTGCGTGCTTTCGGCGATGTC‑TTTTTTATTTGtt                                                                               >  1:57046/1‑174 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  AGCGCCGCTACCGCAAGCCTGGGTTTCGCCGGTACCGCGTTCGTATACGCGTAGTTTGATGTGATGACGATTCACCACCTGCATAAAGCCCGCATTCACCCGTTCCGGAAAACGTTCATGATTTTCCAATAGCGGACCGAGTTGTTCCACATTGGCGGTTTGAATATCCTCCACCTGCACCACGCAATGCGGATTGCCCATAGATACCGCGCTGCACAGCACCGTTTGGATTTCCGTGCGCAGAATATAATTTTTCTCAAATTTATTGGC‑GGT‑AAAC‑GGAATTTTATTCGGCTCCCAAATCGGTTCGCCCATGTTCACCCGCACGTTACCATCTTC‑TTTCACGGTCAGCAGCATTTTGCCGTTTTGCGTGCTTACGGCGATGTC‑TTTTTTATTGGTTAAGCCTTTGAGCGTCACGAAACGGGCAAAACAGCGTGCGCCGTTGCCACATTGCGCCACTTCGCTGCCGTCCGCGTT  >  1/541117‑541590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑