Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	512,736	G→C	intergenic (+149319/‑59283)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	512,736	0	G	C	100.0%	90.3 / NA	32	intergenic (+149319/‑59283)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/11);  total (21/11)

GTAATTCAATGAATGCATTCATTTTTAGCCTCACGGAAATTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGAGTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCTTTCGGGGGATATTGCTTGGGGCGCTGATTACAGTGCTGTTCACTGCATCTAATG  >  1/512493‑512967                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        gTAATTCAATGAATGCATTCATTTTTAGCCTCACGGAAATTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAAt                                                                                                                                                                                                                                  >  1:527585/1‑251 (MQ=255)  tAATTCAATGAATGCATTCATTTTTAGCCTCACGGAAATTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAACTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAAtt                                                                                                                                                                                                                                 <  1:312169/251‑1 (MQ=255)            aatgCATTCATTTTTAGCCTCACGGAAATTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAatatat                                                                                                                                                                                                                       <  1:405450/251‑1 (MQ=255)                                       aTTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTtaaataa                                                                                                                                                                                            >  1:767161/1‑251 (MQ=255)                                                tAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAatatat                                                                                                                                                                                                                       <  1:675651/215‑1 (MQ=255)                                                   gTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTaaaa                                                                                                                                                                                                                  >  1:496338/1‑217 (MQ=255)                                                          atACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGatat                                                                                                                                                                                                        >  1:760836/1‑220 (MQ=255)                                                                  ccTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACtgaa                                                                                                                                                                 >  1:417973/1‑251 (MQ=255)                                                                           gTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTg                                                                                                                                                        >  1:340697/1‑251 (MQ=255)                                                                                                           aCTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTAtcgt                                                                                                                         >  1:578106/1‑250 (MQ=255)                                                                                                              atatCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGtt                                                                                                                     >  1:805332/1‑251 (MQ=255)                                                                                                                   ggATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTaaaa                                                                                                                                                                                                                  >  1:867484/1‑153 (MQ=255)                                                                                                                               taaataaaTTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTaaaa                                                                                                                                                                                                                  >  1:656176/1‑141 (MQ=255)                                                                                                                                 aataaatTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGa                                                                                                  >  1:563472/1‑251 (MQ=255)                                                                                                                                    aaatTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAat                                                                                                                                                                                                                           <  1:680493/127‑1 (MQ=255)                                                                                                                                              gCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTAtcgt                                                                                                                         <  1:502252/215‑1 (MQ=255)                                                                                                                                                tCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGc                                                                                   <  1:726317/251‑1 (MQ=255)                                                                                                                                                           ttACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGt                                                                                                                                                   >  1:614510/1‑176 (MQ=255)                                                                                                                                                             aCTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAAttt                                                                      <  1:855924/251‑1 (MQ=255)                                                                                                                                                              cTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACg                                                                                                                                                                                                            >  1:71709/1‑116 (MQ=255)                                                                                                                                                              cTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAAc                                                                                                                                                                     <  1:848818/155‑1 (MQ=255)                                                                                                                                                                    atgcTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGaa                                                               >  1:167227/4‑251 (MQ=255)                                                                                                                                                                    aTACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGaa                                                               >  1:167242/1‑251 (MQ=255)                                                                                                                                                                     tACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAatatat                                                                                                                                                                                                                       <  1:775980/98‑1 (MQ=255)                                                                                                                                                                     tACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGaa                                                               >  1:725850/1‑250 (MQ=255)                                                                                                                                                                              aGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCttt                                                      >  1:813721/1‑250 (MQ=255)                                                                                                                                                                                     cTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAat                                                                                                                                                                                                                           >  1:569017/1‑78 (MQ=255)                                                                                                                                                                                           aTTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCTTTCGGGGGATATTGc                                         <  1:377954/250‑1 (MQ=255)                                                                                                                                                                                                        ccTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACtgaat                                                                                                                                                                >  1:163731/1‑118 (MQ=255)                                                                                                                                                                                                              gCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTAtcgtcg                                                                                                                       >  1:516908/1‑153 (MQ=255)                                                                                                                                                                                                                                gAGTAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCTTTCGGGGGATATTGCTTGGGGCGCTGATTACAGTGCTGTTc               >  1:509195/1‑239 (MQ=255)                                                                                                                                                                                                                                   tAGACGCCAATGAAAGACTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCTTTCGGGGGATATTGCTTGGGGCGCTGATTACAGTGCTGTTCACTGCATCTAATg  <  1:226031/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        GTAATTCAATGAATGCATTCATTTTTAGCCTCACGGAAATTCATAATTAAGTGGATGATACGAAACCTTTAGCGGTTATTTACCTAAGTAGAATTAGGTTTGGTGAACTATATCGGATATTTAAGATAAATAAATTGATATGCTCCAAGATTCCTTACTATTGATACTAAAACAGATGAACTTAAGATTATAACGATGTCCTGCGGCTATTTATTTTGCTACCGAGTAGACGCCAATGAAAGAGTCGTAATTAGAAATATATTAAAAAATACGATATAAATTTAAATAAAATTCAATTTAAGTAAAATAAACTGAATGAATCTTGATTGTTTTATAATTTTTTGCCAAGGTATCGTCGTTTTTTATAAAAAATTAATGAATAGGTATGTTATGCAATCGACAAATTTAAAAGAACTGACCTTTCGGGGGATATTGCTTGGGGCGCTGATTACAGTGCTGTTCACTGCATCTAATG  >  1/512493‑512967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑