Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	504,427	G→A	intergenic (+141010/‑67592)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	504,427	0	G	A	100.0%	129.2 / NA	45	intergenic (+141010/‑67592)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/32);  total (13/32)

TGCCGAAATCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTGGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGGCGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGCCTTAGCCGCAGCAAACAAACGCGTGGCGAACATTCTTGCCAAAGCAGATGTTGCAATCGGTGAGATTA  >  1/504187‑504660                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          tGCCGGAATCGTTGGTGGCAAGTGGGGTGGCATTAGCCGATAAATTCGGCCCCCTTACCGGAATTTTTGGGATCGGGCAAGCGCCGGAAGGCCGTTGCGGCCCCTTCGGTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGAc                                                                                                                                                                                                                                 <  1:347587/251‑1 (MQ=255)        aTCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGTCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGc                                                                                                                                                                                                                          <  1:219217/251‑1 (MQ=255)         tCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCt                                                                                                                                                                                                                         <  1:456310/251‑1 (MQ=255)         tCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCt                                                                                                                                                                                                                         <  1:43033/251‑1 (MQ=255)         tCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCt                                                                                                                                                                                                                         <  1:698267/251‑1 (MQ=255)                 ggCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACttt                                                                                                                                                                                                                              >  1:642996/1‑238 (MQ=255)                       tGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCcg                                                                                                                                                                                                             <  1:626257/249‑1 (MQ=255)                         cGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGAAGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCcgtgc                                                                                                                                                                                                          <  1:95627/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:243687/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:72524/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:3606/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:351353/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:2025/250‑1 (MQ=255)                                     ccGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTCACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:53331/250‑1 (MQ=255)                                      cGATAAATTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGATCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACg                                                                                                                                                                                            <  1:168007/251‑1 (MQ=255)                                           aaTTCGACACCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGc                                                                                                                                                                                       <  1:726351/251‑1 (MQ=255)                                                  cacCCTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCgg                                                                                                                                                                                <  1:349495/251‑1 (MQ=255)                                                    cccTCACAGGAATTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTg                                                                                                                                                                              >  1:807955/1‑251 (MQ=255)                                                              aaTTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATcc                                                                                                                                                                    <  1:247349/251‑1 (MQ=255)                                                              aaTTTTTGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATc                                                                                                                                                                     <  1:375133/250‑1 (MQ=255)                                                                  tttgggATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGc                                                                                                                                                                <  1:859426/248‑1 (MQ=255)                                                                      ggATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGAc                                                                                                                                                                           <  1:471500/236‑1 (MQ=255)                                                                      ggATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATc                                                                                                                                                                     >  1:815161/1‑242 (MQ=255)                                                                             aCAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGcc                                                                                                                                                      <  1:874460/250‑1 (MQ=255)                                                                             aCAAGCGCCGAAAGGCAGTGCCCACCCATTCGCTTTACGCCGTGCCGGATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGGGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGcc                                                                                                                                                      <  1:670864/250‑1 (MQ=255)                                                                                          ggCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCg                                                                                                                                        >  1:369832/1‑251 (MQ=255)                                                                                              gTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATc                                                                                                                                                                     <  1:70690/218‑1 (MQ=255)                                                                                              gTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGcc                                                                                                                                      >  1:224206/1‑249 (MQ=255)                                                                                                                             aTTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTAt                                                                                                     <  1:180789/251‑1 (MQ=255)                                                                                                                                     cgTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCcgtg                                                                                                                                                                                                           <  1:329891/141‑1 (MQ=255)                                                                                                                                     cgTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGc                                                                                                                                                                >  1:479672/1‑184 (MQ=255)                                                                                                                                     cgTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGc                                                                                                                                                                >  1:174959/1‑184 (MQ=255)                                                                                                                                         aCGCATTATTGTTGAGAAAAACCTACCGCTTGGTTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGCCTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGccc                                                                                                                                              <  1:395234/198‑1 (MQ=255)                                                                                                                                         aCGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTATTGCAAAAATCAGCCGCCCATTTCGGCGATAAACTCTCCAACAAAAATGTGGTTGCCGACGTGGTGGTCTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACg                                                                                                                                                                          <  1:320189/170‑1 (MQ=255)                                                                                                                                          cGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCAc                                                                                        <  1:811736/251‑1 (MQ=255)                                                                                                                                            cATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGc                                                                                                                                                                <  1:160001/177‑1 (MQ=255)                                                                                                                                              ttattGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGa                                                                                                                                                                                              <  1:475767/145‑1 (MQ=255)                                                                                                                                              ttattGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGccc                                                                                                                                                     <  1:299475/186‑1 (MQ=255)                                                                                                                                              ttattGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTa                                                                                    >  1:817240/1‑251 (MQ=255)                                                                                                                                                 ttgttgAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCTCTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGAt                                                                                 <  1:206354/251‑1 (MQ=255)                                                                                                                                                                         aTTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGc                                                                                                                                                                >  1:434471/1‑148 (MQ=255)                                                                                                                                                                         aTTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGCCTTAGCCgcagca                                                         >  1:690569/1‑251 (MQ=255)                                                                                                                                                                             ggAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGCCTTAGCCGCAGcaaaca                                                     >  1:694249/1‑251 (MQ=255)                                                                                                                                                                                                                               acaaAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGAGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGcc                                                                     >  1:476816/1‑185 (MQ=255)                                                                                                                                                                                                                                caaAAATGTGGTTGCCGACGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGCCTTAGCCGCAGCAAACAAACGCGTGGCGAACATTCTTGCCAAAGCAGATGTTGCAATCGGTGAGATTa  >  1:115807/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          TGCCGAAATCGTTGGTGGCAAGTGCGGTGGCATTAGCCGATAAATTCGACACCCTCACAGGAATTTTGGGGATCGGACAAGCGCCGAAAGGCAGTGCCGACCCATTCGCTTTACGCCGTGCCGCATTGGGCGCGTTACGCATTATTGTTGAGAAAAACCTACCGCTTGATTTGGAAGATTTAGTGCAAAAATCAGCCGCACTTTTCGGCGATAAACTCACCAACAAAAATGTGGTTGCCGGCGTGGTGGACTTTATGCTCGGTCGTTTCCGTGCGTGGTATCAAGACGAAGGCATTGCGGTGGACGTCATCCAAGCCGTGCTTGCCCGTCGCCCAACCCGCCCTGCGGATTTTGACGCCCGCGTGCGTGCCGTATCGCATTTCCGCACCTTAGATTCTGCAGAAGCCTTAGCCGCAGCAAACAAACGCGTGGCGAACATTCTTGCCAAAGCAGATGTTGCAATCGGTGAGATTA  >  1/504187‑504660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑