Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	462,618	G→A	intergenic (+99201/‑109401)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	462,618	0	G	A	100.0%	84.5 / NA	46	intergenic (+99201/‑109401)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

CACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAGATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCT  >  1/462379‑462864                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg                                                                                                                                                                                                                                                    >  1:516002/1‑244 (MQ=255)       gATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGAt                                                                                                                                                                                                                                       >  1:342208/1‑251 (MQ=255)               ccccAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaag                                                                                                                                                                                                                                >  1:744744/1‑251 (MQ=255)               ccccAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg                                                                                                                                                                                                                                                    >  1:508810/1‑230 (MQ=255)                           gacacggacacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGa                                                                                                                                                                                                                                        >  1:629087/1‑230 (MQ=16)                                ggacacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATc                                                                                                                                                                                                                 >  1:879860/1‑250 (MQ=11)                                  acacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATccc                                                                                                                                                                                                               <  1:901112/250‑1 (MQ=11)                                     ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaaca                                                                                                                                                                                                         >  1:877292/1‑251 (MQ=9)                                     ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaagaa                                                                                                                                                                                                                              <  1:139465/230‑1 (MQ=9)                                     ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaa                                                                                                                                                                                                           >  1:736451/1‑251 (MQ=11)                                      cTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAAACTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaac                                                                                                                                                                                                          <  1:594780/251‑1 (MQ=11)                                        aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaa                                                                                                                                                                                                        >  1:528141/1‑251 (MQ=11)                                        aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGAGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaagaaag                                                                                                                                                                                                                            <  1:469041/229‑1 (MQ=9)                                         aTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaaca                                                                                                                                                                                                     >  1:851005/1‑251 (MQ=14)                                         aTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaaca                                                                                                                                                                                                     >  1:829844/1‑251 (MQ=14)                                                     gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGGGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCa                                                                                                                                                                                         >  1:250326/1‑251 (MQ=9)                                                     gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaact                                                                                                                                                                                                     <  1:380148/241‑2 (MQ=11)                                                      aTTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTccc                                                                                                                                                                                           <  1:223326/248‑1 (MQ=14)                                                         aTGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg                                                                                                                                                                                     <  1:207233/251‑1 (MQ=14)                                                         aTGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaag                                                                                                                                                                                                                                <  1:404710/210‑1 (MQ=11)                                                           gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGAt                                                                                                                                                                                                                                       <  1:320903/199‑1 (MQ=12)                                                           gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCg                                                                                                                                                                                   >  1:450059/1‑251 (MQ=14)                                                           gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg                                                                                                                                                                                     >  1:897128/1‑251 (MQ=11)                                                                actcttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaa                                                                                                                                                                              >  1:366174/1‑251 (MQ=14)                                                                   cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGAc                                                                                                                                                                                                  <  1:656815/228‑1 (MQ=14)                                                                   cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaaaaa                                                                                                                                                                           >  1:732945/1‑251 (MQ=14)                                                                   cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTCCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaaaaa                                                                                                                                                                           >  1:297610/1‑251 (MQ=14)                                                                   cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg                                                                                                                                                                                     >  1:249516/1‑241 (MQ=14)                                                                   cttacCAATTGCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg                                                                                                                                                                                                                                                    <  1:454698/180‑1 (MQ=11)                                                                   cttacCAACTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGa                                                                                                                                                                                                                                                 <  1:779677/181‑1 (MQ=17)                                                                     tacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAg                                                                                                                                                                          >  1:80582/1‑250 (MQ=14)                                                                                                                                                                                                                                              ccTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAcc   <  1:16779/251‑1 (MQ=11)                                                                                                                                                                                                                                              ccTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAcc   <  1:613549/251‑1 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAGAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:140638/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAACAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:446918/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:546296/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:214597/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:852901/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:286081/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTAc    >  1:271922/1‑249 (MQ=11)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:813532/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:829634/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:500437/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:459084/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTGCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:33530/251‑1 (MQ=14)                                                                                                                                                                                                                                               cTAAAATAATGAAGCCAAAGAAGAAAAAGAAAGCGAATGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAAAGCTGGCATTAACACGTGCACAGAATGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCt  <  1:517408/251‑1 (MQ=11)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAGATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAGCTGCAACGAGAGCTGGCATTAGCACGTGCACAGATTGCTTACCTAAAGGAGTTGGAGGCACTCGACCGTCAAAAACAGCGACAGAAAAAAGAAAAATCATTGAAAGATTGAAGCCAAATCACGCGTTAAATGACTTACTTCGTGTGGCTAAAATGCCTCGTTCTACCT  >  1/462379‑462864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑