| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 462,512 | T→G | intergenic (+99095/‑109507) | unknown → / → unknown | tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 462,512 | 0 | T | G | 93.4% | 161.0 / 3.2 | 76 | intergenic (+99095/‑109507) | unknown/unknown | tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (39/32); total (41/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01 | |||||||||||
AATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAGATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAG > 1/462267‑462696 | aaTTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGaaact < 1:211689/251‑3 (MQ=255) cacCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAGGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGtt > 1:275174/1‑251 (MQ=255) cacCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGtt > 1:215795/1‑251 (MQ=255) gCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTAt > 1:341581/1‑251 (MQ=255) aaaTGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑a < 1:56730/251‑1 (MQ=255) ggCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGcc < 1:130955/249‑1 (MQ=255) ggCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCtt > 1:858432/1‑251 (MQ=255) cacaCCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGcc > 1:68607/1‑243 (MQ=255) acacCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGaa < 1:580042/251‑1 (MQ=255) tCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAg > 1:110595/1‑196 (MQ=255) tCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAAt > 1:26391/1‑251 (MQ=255) cTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc < 1:254574/251‑1 (MQ=255) cTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc < 1:427356/251‑1 (MQ=255) ttCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTaaaat < 1:828780/251‑4 (MQ=255) ttCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTaaaat < 1:185103/251‑4 (MQ=255) gCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat > 1:503364/1‑222 (MQ=255) cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAccc < 1:9422/227‑1 (MQ=255) cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg > 1:516002/1‑244 (MQ=255) cACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACAAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCAGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGCAAGAGGGCTTAAt > 1:190524/1‑201 (MQ=255) aCTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc < 1:829796/225‑1 (MQ=255) cTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGc > 1:571814/1‑194 (MQ=255) gATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGAt > 1:342208/1‑251 (MQ=255) gCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc < 1:411520/215‑1 (MQ=255) ccccAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAAt > 1:783098/1‑187 (MQ=255) ccccAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaag > 1:744744/1‑251 (MQ=255) ccccAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg > 1:508810/1‑230 (MQ=255) gacacggacacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGa > 1:629087/1‑230 (MQ=16) ggacacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATc > 1:879860/1‑250 (MQ=11) gacacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAc > 1:99360/1‑193 (MQ=21) acacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATccc < 1:901112/250‑1 (MQ=11) cacCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGc < 1:460274/162‑1 (MQ=25) ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaaca > 1:877292/1‑251 (MQ=9) ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑aaaa > 1:71407/1‑174 (MQ=21) ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaagaa < 1:139465/230‑1 (MQ=9) ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat > 1:578082/1‑187 (MQ=11) ccTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaa > 1:736451/1‑251 (MQ=11) cTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAAACTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaac < 1:594780/251‑1 (MQ=11) aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAag < 1:566356/152‑1 (MQ=255) aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat > 1:695174/1‑182 (MQ=21) aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaa > 1:528141/1‑251 (MQ=11) aaTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGAGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaagaaag < 1:469041/229‑1 (MQ=9) aTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaaca > 1:851005/1‑251 (MQ=14) aTTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaaca > 1:829844/1‑251 (MQ=14) gTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTtcataaaatctcc < 1:877259/105‑10 (MQ=14) gTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTaaaat < 1:218990/197‑4 (MQ=11) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGGGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCa > 1:250326/1‑251 (MQ=9) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat < 1:762231/169‑1 (MQ=25) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAccc > 1:372101/1‑175 (MQ=21) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAag > 1:235025/1‑141 (MQ=2) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAccc > 1:384126/1‑177 (MQ=11) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTaaaat > 1:652554/1‑189 (MQ=11) gATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGaacaaact < 1:380148/241‑2 (MQ=11) aTTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTccc < 1:223326/248‑1 (MQ=14) aTGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg < 1:207233/251‑1 (MQ=14) aTGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAaaag < 1:404710/210‑1 (MQ=11) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat < 1:161160/163‑1 (MQ=25) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc > 1:68552/1‑168 (MQ=25) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGAt < 1:320903/199‑1 (MQ=12) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCg > 1:450059/1‑251 (MQ=14) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAag < 1:377876/135‑1 (MQ=2) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAcc > 1:880063/1‑170 (MQ=11) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAAc > 1:217527/1‑176 (MQ=11) gTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg > 1:897128/1‑251 (MQ=11) actcttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTaaaat < 1:316459/179‑4 (MQ=12) actcttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaa > 1:366174/1‑251 (MQ=14) cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtatat < 1:244382/155‑1 (MQ=35) cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGAc < 1:656815/228‑1 (MQ=14) cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaaaaa > 1:732945/1‑251 (MQ=14) cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTCCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTaaaaa > 1:297610/1‑251 (MQ=14) cttacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAg > 1:249516/1‑241 (MQ=14) cttacCAATTGCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg < 1:454698/180‑1 (MQ=11) cttacCAACTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGa < 1:779677/181‑1 (MQ=17) tacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCAtt < 1:900396/99‑1 (MQ=255) tacCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAg > 1:80582/1‑250 (MQ=14) ccAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAAccc < 1:249728/157‑1 (MQ=16) gTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCGAAACTTCATAAAATCTCCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTtata < 1:892280/144‑1 (MQ=11) | AATTATCGCGACCGGCACACCGGAACAGGTTGCCAAAATGGAGGGCTCGCACACCGCCCGCTTCCTCAAAGAGATTTTGGCAAAAGGCTAAAAACGCTGCATTTTCCGACCGCACTTTGATCTGCACCCCAAAACCTGGACACGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGT‑AAAATCCCCTTATATAAACCCTCAACGCCCTAAGATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACAGACTTTTCCCCAAAAGCGTTTAAAAAG > 1/462267‑462696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |