| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 443,485 | T→G | intergenic (+80068/‑128534) | unknown → / → unknown | tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 443,485 | 0 | T | G | 100.0% | 106.9 / NA | 36 | intergenic (+80068/‑128534) | unknown/unknown | tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (21/15); total (21/15) | |||||||||||
AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGCTTCATTCATTTGCCGAACCT > 1/443337‑443729 | aaaCGGCGGGTTAATACTCCGCCGTTTTTCTTTTCATGTACATGATGATTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaatgt < 1:82718/203‑12 (MQ=255) tgatgaTTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAacac > 1:770647/1‑149 (MQ=255) gctttAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaa > 1:450714/4‑140 (MQ=255) gcgctatttggcgttaaaccacataagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggtta > 1:842229‑M1/16‑90 (MQ=255) ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca > 1:419408‑M1/13‑87 (MQ=255) ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTCATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca > 1:174329‑M1/13‑87 (MQ=255) atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca > 1:111497‑M1/11‑85 (MQ=255) atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca > 1:235911‑M1/11‑85 (MQ=255) atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgc > 1:783122‑M1/11‑85 (MQ=255) tggcgttaaaccacataAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc < 1:599060‑M1/244‑170 (MQ=255) gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc > 1:88256‑M1/6‑80 (MQ=255) gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc > 1:880404‑M1/6‑80 (MQ=255) gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc > 1:57300‑M1/6‑80 (MQ=255) gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc > 1:268599‑M1/6‑80 (MQ=255) ccacataagttTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgggaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaaca > 1:450349‑M1/5‑72 (MQ=255) cataaGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacatta < 1:346124‑M1/250‑183 (MQ=255) aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct < 1:410578‑M1/248‑186 (MQ=255) aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct < 1:849248‑M1/248‑186 (MQ=255) aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct < 1:643011‑M1/248‑186 (MQ=255) aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct < 1:743567‑M1/248‑186 (MQ=255) gtttaGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcg > 1:189643‑M1/2‑64 (MQ=255) gtttaGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaat > 1:401336‑M1/2‑64 (MQ=255) tttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctc < 1:42191‑M1/249‑187 (MQ=255) tttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctc < 1:274764‑M1/249‑187 (MQ=255) tagaaatgttgtttctaggtttatgTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcgggg > 1:838498‑M1/12‑61 (MQ=255) tagaaatgttgtttctaggtttatgTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcgggg < 1:708790‑M1/240‑191 (MQ=255) tgttgtttctaggTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagt < 1:791297‑M1/100‑51 (MQ=255) gttgtttctagGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttat > 1:135142‑M1/5‑54 (MQ=255) gtttcTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggg > 1:501880‑M1/2‑51 (MQ=255) gtttcTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatgga > 1:607724‑M1/2‑51 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:6807‑M1/251‑202 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:248526‑M1/251‑202 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:674489‑M1/251‑202 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:844343‑M1/251‑202 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:39907‑M1/251‑202 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtc > 1:178814‑M1/1‑50 (MQ=255) tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgtagatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa < 1:179977‑M1/251‑202 (MQ=255) ttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttttgggaa > 1:303294‑M1/1‑49 (MQ=255) ttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccgg > 1:209444‑M1/1‑49 (MQ=255) | AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGCTTCATTCATTTGCCGAACCT > 1/443337‑443729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |