Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	415,272	G→A	intergenic (+51855/‑156747)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	415,272	0	G	A	100.0%	200.9 / NA	71	intergenic (+51855/‑156747)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/33);  total (38/33)

AGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAGTAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCCAC  >  1/415025‑415503                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        aGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATaa                                                                                                                                                                                                                                      >  1:108038/1‑251 (MQ=255)  gTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATtaaataaa                                                                                                                                                                                                                                     >  1:744643/1‑251 (MQ=255)    ggATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   >  1:723545/1‑251 (MQ=255)    ggATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   >  1:797549/1‑251 (MQ=255)     gATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   <  1:898013/250‑1 (MQ=255)      aTAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGaa                                                                                                                                                                                                                                 <  1:570282/251‑1 (MQ=255)                ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       <  1:755493/251‑1 (MQ=255)                ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:766570/1‑251 (MQ=255)                ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:78862/1‑251 (MQ=255)                          gCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAg                                                                                                                                                                                                             >  1:66732/1‑251 (MQ=255)                           cccTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAg                                                                                                                                                                                                             <  1:214086/250‑1 (MQ=255)                                             aaCTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGc                                                                                                                                                                                           <  1:581317/250‑1 (MQ=255)                                               cTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGa                                                                                                                                                                                        >  1:499886/1‑251 (MQ=255)                                               cTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGa                                                                                                                                                                                        >  1:412087/1‑251 (MQ=255)                                                   tGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTcc                                                                                                                                                                                                                            >  1:19098/1‑211 (MQ=255)                                                             gAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTa                                                                                                                                                                          >  1:541725/1‑251 (MQ=255)                                                              aaaaaaaCGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATc                                                                                                                                                                             <  1:614301/247‑1 (MQ=255)                                                                           tAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGc                                                                                                                                                                                           >  1:880790/1‑220 (MQ=255)                                                                            aaTTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTg                                                                                                                                                                                                                          >  1:81977/1‑188 (MQ=255)                                                                             aTTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTGAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTc                                                                                                                                                              >  1:454152/1‑247 (MQ=255)                                                                                  ggATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATAc                                                                                                                                                                                     <  1:48308/219‑1 (MQ=255)                                                                                    aTGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGgt                                                                                                                                                   <  1:884883/251‑1 (MQ=255)                                                                                                ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGTTGTTCGCCAGTGGGTTACTTCCTTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTgctgtctcttatccaaatct                                                                                                                                        >  1:305206/1‑231 (MQ=255)                                                                                                ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAg                                                                                                                                       >  1:614524/1‑251 (MQ=255)                                                                                                ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAg                                                                                                                                       >  1:845317/1‑251 (MQ=255)                                                                                                  aaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     <  1:763882/251‑1 (MQ=255)                                                                                                          gCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTCCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGgtg                                                                                                                                                  <  1:452089/230‑1 (MQ=255)                                                                                                           cGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTaaga                                                                                                                            <  1:171472/251‑1 (MQ=255)                                                                                                                   ggATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:349068/1‑152 (MQ=255)                                                                                                                           ctctcGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCt                                                                                                                                          <  1:878161/221‑1 (MQ=255)                                                                                                                             ctcGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAAttt                                                                                                                                                                                                                              <  1:169728/135‑1 (MQ=255)                                                                                                                                gAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGc                                                                                                                                           >  1:477841/1‑215 (MQ=255)                                                                                                                                 aaaaTATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACt                                                                                                       <  1:64801/250‑1 (MQ=255)                                                                                                                                 aaaaTATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTa                                                                                                      >  1:440496/1‑251 (MQ=255)                                                                                                                                       tCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGt                                                                                                                                                          <  1:658127/193‑1 (MQ=255)                                                                                                                                            gcgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGaa                                                                                           >  1:582911/1‑251 (MQ=255)                                                                                                                                             cgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAAc                                                                                          <  1:57372/251‑1 (MQ=255)                                                                                                                                             cgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCAGTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAAc                                                                                          <  1:424681/251‑1 (MQ=255)                                                                                                                                                 gAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTa                                                                                      >  1:809739/1‑251 (MQ=255)                                                                                                                                                    gTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTc                                                                                                                                                                                                  >  1:487178/1‑140 (MQ=255)                                                                                                                                                     ttCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAggg                                                                                   <  1:287340/250‑1 (MQ=255)                                                                                                                                                       cAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGtt                                                                                 >  1:776930/1‑250 (MQ=255)                                                                                                                                                            ccTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:83039/1‑111 (MQ=255)                                                                                                                                                             cTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          <  1:236441/251‑1 (MQ=255)                                                                                                                                                               gagaATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgaccg                                                                        >  1:255746/1‑251 (MQ=255)                                                                                                                                                                   aTTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGgtg                                                                                                                                                                                                                     <  1:376663/106‑1 (MQ=255)                                                                                                                                                                             gATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACgg                                                          >  1:584311/1‑251 (MQ=255)                                                                                                                                                                              aTTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGtt                                                                                                                                                                <  1:760552/148‑1 (MQ=255)                                                                                                                                                                                        aCTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCg                                                   <  1:721832/247‑1 (MQ=255)                                                                                                                                                                                        aCTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGT‑CCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     >  1:11675/1‑164 (MQ=255)                                                                                                                                                                                        aCTAAAAGTATTCTGAGAAAAAGAATTTGAGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGt                                               >  1:851017/1‑251 (MQ=255)                                                                                                                                                                                            aaaGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGCAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGtctc                                            >  1:438076/1‑250 (MQ=255)                                                                                                                                                                                               gTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          >  1:123885/1‑217 (MQ=255)                                                                                                                                                                                                tATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTAc                                        <  1:774983/250‑1 (MQ=255)                                                                                                                                                                                                 aTTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAg                                                                                     >  1:805181/1‑204 (MQ=255)                                                                                                                                                                                                 aTTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCa                                      >  1:630776/1‑251 (MQ=255)                                                                                                                                                                                                    cTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAaat                                   <  1:227094/251‑1 (MQ=255)                                                                                                                                                                                                      gagaAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     <  1:222091/151‑1 (MQ=255)                                                                                                                                                                                                      gagaAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGga                                                                           <  1:382428/209‑1 (MQ=255)                                                                                                                                                                                                              gAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       <  1:454605/61‑1 (MQ=255)                                                                                                                                                                                                                    gtgCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGca                   >  1:317893/1‑251 (MQ=255)                                                                                                                                                                                                                          gTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTaaga                                                                                                                            <  1:734565/140‑1 (MQ=255)                                                                                                                                                                                                                           tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCaa                      >  1:806168/1‑241 (MQ=255)                                                                                                                                                                                                                           tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATcc              <  1:407987/249‑1 (MQ=255)                                                                                                                                                                                                                           tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCtt            >  1:37247/1‑251 (MQ=255)                                                                                                                                                                                                                               tAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAAc        <  1:536863/251‑1 (MQ=255)                                                                                                                                                                                                                                     tAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCcac  <  1:587039/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          <  1:728872/177‑1 (MQ=255)                                                                                                                                                                                                                                                 gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATc                                                                                                                                                                             >  1:57258/1‑68 (MQ=255)                                                                                                                                                                                                                                                 gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTcc                                                                                                                                                             >  1:685416/1‑84 (MQ=255)                                                                                                                                                                                                                                                 gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTc                                                                                                                                    <  1:423963/109‑1 (MQ=255)                                                                                                                                                                                                                                                 gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCa          >  1:370203/1‑231 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        AGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAGTAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCCAC  >  1/415025‑415503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑