| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 23,097 | G→A | intergenic (+18490/+107408) | unknown → / ← unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 23,097 | 0 | G | A | 89.9% | 190.1 / ‑2.8 | 79 | intergenic (+18490/+107408) | unknown/unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/50); minor base C (8/0); total (29/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCGCCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCACCGCACTTTTTTA > 1/22852‑23328 | aCGGTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCtt < 1:255745/250‑1 (MQ=255) cGGTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAg < 1:209658/251‑1 (MQ=255) gTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCATAGAGACGGGCAATCCACCCTAGt > 1:50806/1‑250 (MQ=255) gTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGATTGAATCAGCCCCATAAAGACCGGCCATCCACCtttct > 1:354775/1‑247 (MQ=255) tGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTcgccg > 1:357808/1‑246 (MQ=255) cGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTCCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATc < 1:423091/251‑1 (MQ=255) cATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCaa < 1:685744/250‑1 (MQ=255) cGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAAACCACCtt > 1:131222/1‑206 (MQ=255) ttGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGt < 1:734783/251‑1 (MQ=255) tGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCa < 1:124524/251‑1 (MQ=255) aaGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTcc < 1:549823/251‑1 (MQ=255) aGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGGTGCAGCCGCCCAGAGACGGGCACTCCCCCTCAGTCGCCGCCGTCGGGCAATATCAAAGCGACACCGCC‑GTAGCCGTGGGTTACTCACGCAATTcc > 1:4880/1‑250 (MQ=255) aGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCACAGAAAACGGCAATCCACCCTAGGCGCCCCGGCCGGTCAAAAACAAAAGGAAACCGCC‑GTCGATGTgggcgcctcccgcgtttcc > 1:425813/1‑234 (MQ=255) ggAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGa < 1:496779/250‑1 (MQ=255) gCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTc < 1:67660/242‑1 (MQ=255) cACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACgg < 1:870733/250‑1 (MQ=255) cGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGaaaa < 1:288101/251‑1 (MQ=255) gACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCATAGAGACGGGCAATCCCCCCTAGTCGCCGCCGTCGGGCAATATCAAAACGAAAACGCT‑GTAGCTGGGGGGTACTCACGCATTTTCGATAAAGGaaaaa > 1:393284/1‑250 (MQ=255) aCGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAAt < 1:681813/250‑1 (MQ=255) cGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATAc < 1:363833/251‑1 (MQ=255) aTGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGTTGCAGCCCCCTAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGGCAATATCAAAGCGAAAACGCC‑GTAGCCGTGGGGTACTCACCCATTTCCCATAACGGGAAATACggg > 1:395/1‑250 (MQ=255) cAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCCTAGAGACGGGCAATCCACCTTAGTCGCCGCCGTCGGGCAATATCAAAGCGAAACCGCC‑GTAGCTGTGGGGTACTCACGCATTTCCGATAAAGGAAAATACGGC‑ggg > 1:507807/1‑249 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCCCCTTAGTCGCCGCCGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGGGGGGTACTCACGCATTTCCGATAACGGAAAAAACGGC‑ggggaaa > 1:502976/1‑245 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCaa < 1:797503/187‑1 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAg < 1:800386/251‑1 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAACGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTCCGATAACGGAAAATAc > 1:464419/1‑241 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCCCATAAAGACGGGCCATCCCCCCTAGTCGCCGCCGTCGGTCAATATCAAAGCGAAACCCCT‑GTAGCTGTGGGTTTCTCACGCATTTCCGATAACGGAAAAAACGGC‑G‑Tggaaa > 1:894000/1‑247 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCATAGAGACGGGCAATCCCCCTTAGTCGCCGCTGGCGGGCAATATCAAAACGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTCCGATAACGGAAAAAACGGC‑ggggaaa > 1:542979/1‑245 (MQ=255) aaaTATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAACGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTTCGATAACGGAAAAAACGGC‑G‑TGAAAg > 1:365443/1‑251 (MQ=255) gTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATAc < 1:283892/233‑1 (MQ=255) cGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCCCCTTTGTCGCCGCCGTCGGTCAATATCAAAACGAAAACGCTGGTGGC‑GTGGGGTACTCACGCATTTTCGATAAAGGGAAATAAGGCGG‑GGAAGGTTTTTTTC‑GCACCAAcacc > 1:825543/1‑247 (MQ=255) cGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTca‑c < 1:651752/251‑1 (MQ=255) tCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑Caa < 1:375356/251‑1 (MQ=255) tCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑Caa < 1:267513/251‑1 (MQ=255) gCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGGAAACCACCCTTGTCGCCGCCGTCGGGCAATATTAAAGGCAAACCGCT‑GTAGCTCTGGGTTACTCACGCATTTTCGATAACGGAAAAAAAGGC‑G‑CGAAAATTTTTTTTACCACCAACTca‑cacaaggaaat > 1:20735/1‑242 (MQ=255) gCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCCCCTTAGTCGCCGCCGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAAAACGGCGG‑GGAAAGttttttttgcccccacccccaaagggaagg > 1:132951/1‑228 (MQ=255) gCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGGTTTATGCAGCCGCATAGAGACGGGCAAACCACCCTAGTCGCCGCTGTCGGTCAATATCAAAACGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTTCGATAACGGAAAAAAAGGC‑G‑GGAAAGATTTTTTTAGCAcccacccaaaaaggggaaa > 1:59288/1‑234 (MQ=255) aTTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGtt < 1:337492/249‑1 (MQ=255) attgataacattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAgg < 1:372094/251‑1 (MQ=255) acattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGGTGCAGCCGCATAGAGACGGGCAATCCACCCTAGTCGCCCCTGTCGGTCAATATCAAAACGAAACCGCT‑GTAGCTGTGGGGTACTCACGCATTTCCGATAAAGGAAAAAACGGC‑G‑GGGAAGTTTTTTTTATCACCCACTCA‑CAAAGTGGAGTTGGCGGGACGGCAgggggcggg > 1:548816/1‑244 (MQ=255) acattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAAt < 1:262504/186‑1 (MQ=255) acattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCCTAGTCGCCGCTGTCGGGCAATATCAAAACGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTCCGATAAAGGAAAAAACGGC‑G‑TGGAAGGTTTTTTTAGCACCCACCCACCAAGGGGAAG‑TGGGGGGACGGCAGGCGCgggg > 1:534534/1‑250 (MQ=255) cattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCCCCTTAGTCGCCGCTGTCGGGCAATATCAAAGCGAAACCGCT‑GTAGCTGGGGGGTACTCACGCATTTTCGATAACGGAAAATAAGGC‑G‑GGGAAGATTTTTTTTGCACCCACACA‑CAAGGGGGAGTTggggggagggggggggcggggg > 1:339014/1‑231 (MQ=255) cattgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATc < 1:598511/131‑1 (MQ=255) attgataaGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATAc < 1:54089/186‑1 (MQ=255) cgcgTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGt < 1:479006/250‑1 (MQ=255) aaaaaaaTGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATg < 1:864342/250‑1 (MQ=255) aaaTGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGt < 1:526843/240‑1 (MQ=255) aaTGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTa < 1:34414/251‑1 (MQ=255) gATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGt < 1:590891/203‑1 (MQ=255) gATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTc < 1:243834/250‑1 (MQ=255) gATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTc < 1:46862/250‑1 (MQ=255) gTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAAAGACGGGCAATCCACCCTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAAACGCT‑GTAGCTGTGGGGTACTCACGCCTTTCCGATAACGGAAAATACGGC‑GCGGAAATTTTTTTTC‑GCCCCAACCCA‑CCAAGTGAAGTTGGCGGGGCGGCCGGGGCGGGCTAATTCTTGTAAAAAGCGCttcttttactaaa > 1:685216/1‑239 (MQ=255) tCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCCCCTAGAGACGGGGAATCCACCCTAGTCGCCCCTGTCGGGCCATATCAAAACGAAAACGCC‑GTAGCTGTGGGGTTCTCCCGCCTTTCCCCTAACGGAAAAAACGGC‑G‑CGAAAGGTTCTTTTAGCACCCACCCA‑CAAAGGGAAGGTGGCGGTACGGCAAGCGCGGGCGTTTTCTggggaaaaaagctttttttgctaaac > 1:888135/1‑226 (MQ=255) aaGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAAc < 1:18936/251‑1 (MQ=255) aaGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAAc < 1:706211/251‑1 (MQ=255) gCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAg < 1:790400/250‑1 (MQ=255) cACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTg < 1:849966/251‑1 (MQ=255) cccTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGtt < 1:165173/173‑1 (MQ=255) ccTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAaat < 1:819910/251‑1 (MQ=255) tCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATAc < 1:614415/128‑1 (MQ=255) cggGTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTttc < 1:771411/250‑1 (MQ=255) gTTTGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCa > 1:832906/1‑251 (MQ=255) tttGATGCAGCCGCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCAc < 1:456763/251‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTc < 1:292373/78‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAg < 1:81676/113‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATTCGCTTATTCAGCTAACCTGAAACAAAAATGCGGGGGAAAAATAATGTGTTTCTTCACCCCCCttttttt > 1:779922/1‑250 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAAcc < 1:838224/199‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAaat > 1:829119/1‑224 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTttc < 1:641598/234‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCACCGCACTTTTTTa > 1:842451/1‑251 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCCTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCAc < 1:645804/239‑1 (MQ=255) gCATAGAGACGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGGGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTTAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCACCGCCCttttttt > 1:870257/1‑250 (MQ=255) gagaCGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGc < 1:97023/208‑1 (MQ=255) gagaCGGGCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAaataa < 1:729471/221‑1 (MQ=255) gggCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATgtgt < 1:205275/221‑1 (MQ=255) ggCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAAtg > 1:154570/1‑217 (MQ=255) ggCAATCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCACCGCACttt < 1:574751/236‑1 (MQ=255) aTCCACCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTAc < 1:441297/61‑1 (MQ=255) | ACGGTCCAAGCATGACAAGCCACGGCATCAACGCCGGCGGCAAACGAATTGCTAACGTTGCGAAAGGGAAAGCACCGACGGATGCAGTAAATATGAGTCAGCTTCAAGACGTCGGCAGTGCCATTAATAATCGCATTGATAACATTGATAAGCGCGTGAAAAAAATGGATAAACGTCGCAAAGCCGGCACCGCTTCCGCCCTCGCTACGGCGGGTTTGATGCAGCCGCATAGAGACGGGCAATCCGCCTTAGTCGCCGCTGTCGGTCAATATCAAAGCGAAACCGCT‑GTAGCTGTGGGTTACTCACGCATTTCCGATAACGGAAAATACGGC‑G‑TGAAAGTTTCTTTCAGCACCAACTCA‑CAAGGTGAAGTTGGCGGTACGGCAGGCGCGGGCTATTTCTGGTAAAATGCGCTTATTCAGCTAACCTGAAACAAAAGTGCGGTGGAAAAATAATGTGTTTCTTCACCGCACTTTTTTA > 1/22852‑23328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |