Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	325,460	G→A	intergenic (‑40831/‑37867)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	325,460	0	G	A	100.0%	90.6 / NA	33	intergenic (‑40831/‑37867)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

AAAAATTACTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTGCAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAATTTAACGCAAAACCTTGTGATAATTGAAGGAAAACCGCAACTTCAGGGTGACATTTATAT  >  1/325213‑325706                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaaaaTTACTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAg                                                                                                                                                                                                                                                     <  1:392421/251‑1 (MQ=255)    aaTTACTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGaaa                                                                                                                                                                                                                                                  <  1:551825/251‑1 (MQ=255)        aCTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGaaa                                                                                                                                                                                                                                                  <  1:321683/247‑1 (MQ=255)        aCTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAaataat                                                                                                                                                                                                                                              >  1:556422/1‑251 (MQ=255)                       aaGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATAc                                                                                                                                                                                                                                            >  1:697210/1‑238 (MQ=255)                                tGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAatat                                                                                                                                                                                                                      <  1:736659/251‑1 (MQ=255)                                                                   ttCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTa                                                                                                                                                                                   >  1:276360/1‑251 (MQ=255)                                                                                   ttttATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATAc                                                                                                                                                                                                                                            >  1:588927/1‑178 (MQ=255)                                                                                   ttttATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTagaag                                                                                                                                                                                                                              <  1:817282/192‑1 (MQ=255)                                                                                     ttATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACg                                                                                                                                                                                                    <  1:87288/216‑1 (MQ=255)                                                                                          aCCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATa                                                                                                                                                                  <  1:249502/245‑1 (MQ=255)                                                                                                cTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATAc                                                                                                                                                                                                         >  1:583553/1‑200 (MQ=255)                                                                                                        gTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGcc                                                                                                                                                          <  1:177003/239‑1 (MQ=255)                                                                                                          aTTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTAt                                                                                                                                            >  1:824801/1‑251 (MQ=255)                                                                                                             aaGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGa                                                                                                                                         <  1:24530/251‑1 (MQ=255)                                                                                                                                                        aTTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGc                                                                                                                                               >  1:94276/1‑202 (MQ=255)                                                                                                                                                                  gATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCg                                                                                                                                                                           >  1:114821/1‑164 (MQ=255)                                                                                                                                                                     gACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGAt                                                                                                                                                                   <  1:424398/169‑1 (MQ=255)                                                                                                                                                                     gACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAc                                                                                  >  1:376008/1‑250 (MQ=255)                                                                                                                                                                     gACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAc                                                                                  >  1:50682/1‑250 (MQ=255)                                                                                                                                                                      aCTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAcc                                                                                 <  1:332804/250‑1 (MQ=255)                                                                                                                                                                      aCTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAcc                                                                                 <  1:571758/250‑1 (MQ=255)                                                                                                                                                                       cTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGa                                                                                        <  1:160384/242‑1 (MQ=255)                                                                                                                                                                       cTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAc                                                                                  >  1:312254/1‑248 (MQ=255)                                                                                                                                                                             aTGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAAt                                                                         >  1:331700/1‑251 (MQ=255)                                                                                                                                                                              tGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATGCAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAAc                                                                                            <  1:325699/231‑1 (MQ=255)                                                                                                                                                                                cAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGa                                                                       <  1:403026/250‑1 (MQ=255)                                                                                                                                                                                    atcaatcTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATAcc                                                                                 >  1:303425/1‑236 (MQ=255)                                                                                                                                                                                            ctATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAAttt                                                           >  1:716951/1‑250 (MQ=255)                                                                                                                                                                                                                          cTTTTTATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCAGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAATTTAACGCAAAACCTTGTGATAATTGAAGGaaaa                            >  1:432332/1‑251 (MQ=255)                                                                                                                                                                                                                            ttttATCAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGc                                                                                                                                                                                     >  1:407419/1‑96 (MQ=255)                                                                                                                                                                                                                                  cAGTTTATTTTTTGTTTTTCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAATTTAACGCAAAACCTTGTGAt                                         <  1:218693/230‑1 (MQ=255)                                                                                                                                                                                                                                                    tCCTACAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAATTTAACGCAAAACCTTGTGATAATTGAAGGAAAACCGCAACTTCAGGGTGACATTtatat  <  1:402493/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AAAAATTACTCACAATCGAATCAAGTTCGATTGTTCGTCCCAATGGGGCCGTTGACAAAGCCAACTTTCAAAATCCGTTGGATTTTATAACCGCACTTTAGGCGTATTAAGGTGCGGTTAGTTTTTTTGTTATTTTTAAACTTAAAAACCAATTTTAACCGGATGACTTATAATGCAAAATCAATCTCTATTTTCAAATAGAAAATTTCTATTTGCCCTTTTTATCAGTTTATTTTTTGTTTTTCCTGCAGAAATAATACGGATAAATTAGAAGATGAATATCTTGGCGAAATACAAACGAATAGCGAATACCGCTATGTCATCGATATTGATAAATTAGCCAACGGAAAGGCTATTGAAACTTCGGTAAAAATAGCGATTTCACCGGAAAATTCAGGAGTAACAGGAATATACCTGTTCAATGAACAGGCTAATTTAACGCAAAACCTTGTGATAATTGAAGGAAAACCGCAACTTCAGGGTGACATTTATAT  >  1/325213‑325706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑